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HEIDELBERG UNIVERSITY

Walter Nickel 
Unconventional Protein Secretion

Groupleader: Walter Nickel

Selected original publications:

Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W (2017) Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components. eLife (pii: e28985. doi: 10.7554/eLife.28985)

La Venuta G, Wegehingel S, Sehr P, Müller HM, Dimou E, Steringer JP, Grotwinkel M, Hentze N, Mayer MP, Will DW, Uhrig U, Lewis JD, Nickel W (2016) Small Molecule Inhibitors Targeting Tec Kinase Block Unconventional Secretion of Fibroblast Growth Factor 2. J. Biol. Chem. 291:17787-803

Zeitler M, Steringer JP, Müller HM, Mayer MP, Nickel W (2015) HIV-Tat Forms Phosphoinositide Dependent Membrane Pores Implicated in Unconventional Protein Secretion. J. Biol. Chem. 290:21976-84

Müller HM, Steringer JP, Wegehingel S, Bleicken S, Münster M, Dimou E, Unger S, Weidmann G, Andreas H, García-Sáez AJ, Wild K, Sinning I, Nickel W. (2015) Formation of disulfide bridges drives oligomerization, membrane pore formation, and translocation of fibroblast growth factor 2 to cell surfaces. J. Biol. Chem. 290:8925-8937

Zacherl S, La Venuta G, Müller HM, Wegehingel S, Dimou E, Sehr P, Lewis JD, Erfle H, Pepperkok R, Nickel W (2014) A direct role for ATP1A1 in unconventional secretion of fibroblast growth factor 2. J. Biol. Chem. 290:3654-3665

Steringer, JP, Bleicken, S, Andreas, H, Zacherl, S, Laussmann, M, Temmerman, K, Contreras, FX, Bharat, TA, Lechner, J, Müller, HM, Briggs, JA, Garcia, AJ, Nickel, W (2012) PI(4,5)P2 Dependent Oligomerization of Fibroblast Growth Factor 2 (FGF2) Triggers the Formation of a Lipidic Membrane Pore Implicated in Unconventional Secretion. J. Biol. Chem. 2012; 287(33):27659-69.

Antje Ebert, Mareike Laußmann, Sabine Wegehingel, Lars Kaderali, Holger Erfle, Jürgen Reichert, Johannes Lechner, Hans-Dietmar Beer, Rainer Pepperkok and Walter Nickel (2010) Tec kinase mediated phosphorylation of Fibroblast Growth Factor 2 is essential for unconventional secretion. Traffic 11:813-826

Lucía Cespón Torrado, Koen Temmerman, Hans-Michael Müller, Matthias P. Mayer, Claudia Seelenmeyer, Rafael Backhaus, and Walter Nickel (2009) An Intrinsic Quality Control Mechanism Ensures Unconventional Secretion of Fibroblast Growth Factor 2 in a Folded Conformation. J. Cell Sci. 122:3322-3329

Koen Temmerman, Antje D. Ebert, Hans-Michael Müller, Irmgard Sinning, Ivo Tews, and Walter Nickel (2008) A Direct Role for Phosphatidylinositol-4,5-bisphosphate in Unconventional Secretion of Fibroblast Growth Factor 2. Traffic 9:1204-1217

Christoph Zehe, André Engling, Sabine Wegehingel, Tobias Schäfer, and Walter Nickel (2006) Cell surface heparan sulfate proteoglycans are essential components of the unconventional export machinery of FGF-2. Proc. Natl. Acad Sci. U.S.A. 103:15479-15484

Claudia Seelenmeyer, Sabine Wegehingel, Ivo Tews, Markus Künzler, Markus Aebi, and Walter Nickel (2005) Cell Surface Counter Receptors are Essential Components of the Unconventional Export Machinery of Galectin-1. J. Cell Biol. 172:373-381

Tobias Schäfer, Hanswalter Zentgraf, Christoph Zehe, Britta Brügger, Jürgen Bernhagen, and Walter Nickel (2004) Unconventional Protein Secretion of Fibroblast Growth Factor 2 Is Mediated by Direct Translocation across the Plasma Membrane of Mammalian Cells. J. Biol. Chem. 279:6244-6251


Selected review articles:

Brough D, Pellegrin P, and Nickel W (2017) An emerging case for membrane pore formation as a common mechanism for the unconventional secretion of FGF2 and IL-1beta. J. Cell Sci, in press

La Venuta G, Zeitler M, Steringer JP, Müller HM, Nickel W (2015) The Startling Properties of Fibroblast Growth Factor 2: How to Exit Mammalian Cells Without a Signal Peptide at Hand? J Biol Chem. 290:27015-20

Steringer JP, Müller HM, Nickel W (2014) Unconventional Secretion of Fibroblast Growth Factor 2-A Novel Type of Protein Translocation across Membranes? J. Mol. Biol. 427:1202-1210

Rabouille C, Malhotra V, Nickel W. (2012) Diversity in unconventional protein secretion.
J Cell Sci. 125:5251-5.

Walter Nickel (2011) The Unconventional Secretory Machinery of Fibroblast Growth Factor 2. Traffic 12:799-805

Walter Nickel and Catherine Rabouille (2009) Mechanisms of Regulated Unconventional Protein Secretion. Nat. Rev. Mol. Cell Biol. 10:148-155

Walter Nickel and Matthias Seedorf (2008) Unconventional Mechanisms of Protein Transport to the Cell Surface of Eukaryotic Cells. Annu. Rev. Cell Dev. Biol. 24:287-308


Complete list of publications

2017


Brough D, Pellegrin P, and Nickel W (2017) An emerging case for membrane pore formation as a common mechanism for the unconventional secretion of FGF2 and IL-1beta. J. Cell Sci, in press

Steringer JP and Nickel W (2017) The molecular mechanism underlying unconventional secretion of fibroblast growth factor 2 from tumor cells. Biol. Cell, in press

Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W (2017) Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components. eLife (pii: e28985. doi: 10.7554/eLife.28985)

Vanova T, Konecna Z, Zbonakova Z, La Venuta G, Zoufalova K, Jelinkova S, Varecha M, Rotrekl V, Krejci P, Nickel W, Dvorak P, Kunova-Bosakova M (2017) Tyrosine Kinase Expressed in Hepatocellular Carcinoma, TEC, Controls Pluripotency and Early Cell Fate Decisions of Human Pluripotent Stem Cells via Regulation of Fibroblast Growth Factor-2 Secretion. Stem Cells (doi: 10.1002/stem.2660)


2016

La Venuta G, Wegehingel S, Sehr P, Müller HM, Dimou E, Steringer JP, Grotwinkel M, Hentze N, Mayer MP, Will DW, Uhrig U, Lewis JD, Nickel W (2016) Small Molecule Inhibitors Targeting Tec Kinase Block Unconventional Secretion of Fibroblast Growth Factor 2. J. Biol. Chem. 291:17787-803

Martín-Sánchez F, Diamond C, Zeitler M, Gomez AI, Baroja-Mazo A, Bagnall J, Spiller D, White M, Daniels MJ, Mortellaro A, Peñalver M, Paszek P, Steringer JP, Nickel W, Brough D, Pelegrín P. (2016) Inflammasome-dependent IL-1β release depends upon membrane permeabilisation. Cell Death Differ. 23:1219-31

Gerl MJ, Bittl V, Kirchner S, Sachsenheimer T, Brunner HL, Lüchtenborg C, Özbalci C, Wiedemann H, Wegehingel S, Nickel W, Haberkant P, Schultz C, Krüger M, Brügger B. (2016) Sphingosine-1-Phosphate Lyase Deficient Cells as a Tool to Study Protein Lipid Interactions. PLoS One 11:e0153009

2015

La Venuta G, Zeitler M, Steringer JP, Müller HM, Nickel W (2015) The Startling Properties of Fibroblast Growth Factor 2: How to Exit Mammalian Cells Without a Signal Peptide at Hand? J Biol Chem. pii: jbc.R115.689257. [Epub ahead of print]

Zeitler M, Steringer JP, Müller HM, Mayer MP, Nickel W (2015)
HIV-Tat Forms Phosphoinositide Dependent Membrane Pores Implicated in Unconventional Protein Secretion. J Biol Chem [Epub ahead of print]

Stiess M, Wegehingel S, Nguyen C, Nickel W, Bradke F, Cambridge SB (2015)
A Dual SILAC Proteomic Labeling Strategy for Quantifying Constitutive and Cell-Cell Induced Protein Secretion.
J Proteome Res [Epub ahead of print]

Müller HM, Steringer JP, Wegehingel S, Bleicken S, Münster M, Dimou E, Unger S, Weidmann G, Andreas H, García-Sáez AJ, Wild K, Sinning I, Nickel W. (2015) Formation of disulfide bridges drives oligomerization, membrane pore formation, and translocation of fibroblast growth factor 2 to cell surfaces. J. Biol. Chem. 290:8925-8937. 

2014

Zacherl S, La Venuta G, Müller HM, Wegehingel S, Dimou E, Sehr P, Lewis JD, Erfle H, Pepperkok R, Nickel W (2014) A direct role for ATP1A1 in unconventional secretion of fibroblast growth factor 2. J. Biol. Chem. 290:3654-3665

Ugarte-Uribe B, Müller HM, Otsuki M, Nickel W, García-Sáez AJ. (2014) Dynamin-related protein 1 (Drp1) promotes structural intermediates of membrane division. J. Biol. Chem. 289:30645-30656

Steringer JP, Müller HM, Nickel W (2014) Unconventional Secretion of Fibroblast Growth Factor 2-A Novel Type of Protein Translocation across Membranes? J. Mol. Biol. 427:1202-1210

Geist MM, Pan X, Bender S, Bartenschlager R, Nickel W, Fackler OT. (2014) Heterologous Src homology 4 domains support membrane anchoring and biological activity of HIV-1 Nef.
J. Biol. Chem. 289:14030-14044

2013

Disanza A, Bisi S, Winterhoff M, Milanesi F, Ushakov DS, Kast D, Marighetti P, Romet- Lemonne G, Müller HM, Nickel W, Linkner J, Waterschoot D, Ampè C, Cortellino S, Palamidessi A, Dominguez R, Carlier MF, Faix J, Scita G. (2013) CDC42 switches IRSp53 from inhibition of actin growth to elongation by clustering of VASP. EMBO J. 32:2735-50

Bhardwaj R, Müller HM, Nickel W, Seedorf M. (2013) Oligomerization and Ca2+/calmodulin control binding of the ER Ca2+-sensors STIM1 and STIM2 to plasma membrane lipids.
Biosci Rep. 2013 Oct 31;33(5). (pii: e00077. doi: 10.1042/BSR20130089)

Di Ventura B, Knecht B, Andreas H, Godinez WJ, Fritsche M, Rohr K, Nickel W, Heermann DW, Sourjik V. (2013) Chromosome segregation by the Escherichia coli Min system.
Mol Syst Biol. 2013;9:686

Pan X, Geist MM, Rudolph JM, Nickel W, Fackler OT (2013) HIV-1 Nef disrupts membrane- microdomain-associated anterograde transport for plasma membrane delivery of selected Src family kinases. Cell Microbiol. 15:1605-21

Ernst AM, Zacherl S, Herrmann A, Hacke M, Nickel W, Wieland FT, Brügger B. (2013) Differential Transport of Influenza A Neurominidase Signal Anchor Peptides to the Plasma Membrane. FEBS Lett. 587:1411-7

2012

Rabouille C, Malhotra V, Nickel W. (2012) Diversity in unconventional protein secretion.
J Cell Sci. 125:5251-5.

Steringer, JP, Bleicken, S, Andreas, H, Zacherl, S, Laussmann, M, Temmerman, K, Contreras, FX, Bharat, TA, Lechner, J, Müller, HM, Briggs, JA, Garcia, AJ, Nickel, W (2012) PI(4,5)P2 Dependent Oligomerization of Fibroblast Growth Factor 2 (FGF2) Triggers the Formation of a Lipidic Membrane Pore Implicated in Unconventional Secretion. J Biol Chem. 287:27659-69.

Maclean, LM, O'Toole, PJ, Stark, M, Marrison, J, Seelenmeyer, C, Nickel, W, and Smith, DF (2012) Trafficking and release of Leishmania metacyclic HASPB on macrophage invasion.  Cell Microbiol. 14:740-761

Jennemann R, Rabionet M, Gorgas K, Epstein S, Dalpke A, Rothermel U, Bayerle A, van der Hoeven F, Imgrund S, Kirsch J, Nickel W, Willecke K, Riezman H, Gröne HJ, Sandhoff R. (2012) Loss of ceramide synthase 3 causes lethal skin barrier disruption. Hum Mol Genet. 21:586-608

2011

Julia Ritzerfeld, Steffen Remmele, Tao Wang, Koen Temmerman, Britta Brügger, Sabine Wegehingel, Stella Tournaviti, Jeroen R.P.M. Strating, Felix T. Wieland, Beate Neumann, Jan Ellenberg, Chris Lawerenz, Jürgen Hesser, Holger Erfle, Rainer Pepperkok, and Walter Nickel (2011) Phenotypic Profiling of the Human Genome Reveals Gene Products Involved in Plasma Membrane Targeting of SRC Kinases. Genome Res. 21:1955-1968

Thomas Kupke, Leontina Di Cecco, Hans-Michael Müller, Annett Neuner, Frank Adolf, Felix Wieland, Walter Nickel and Elmar Schiebel (2011) Targeting of Nbp1 to the inner nuclear membrane is essential for SPB duplication. EMBO J. 30:3337-3352

Walter Nickel (2011) The Unconventional Secretory Machinery of Fibroblast Growth Factor 2. Traffic 12:799-805

2010

Walter Nickel (2010) Pathways of Unconventional Protein Secretion. Curr. Opin. Biotechnol. 21:621-626

Antje Ebert, Mareike Laußmann, Sabine Wegehingel, Lars Kaderali, Holger Erfle, Jürgen Reichert, Johannes Lechner, Hans-Dietmar Beer, Rainer Pepperkok and Walter Nickel (2010) Tec kinase mediated phosphorylation of Fibroblast Growth Factor 2 is essential for unconventional secretion. Traffic 11:813-826

2009

Ebru Ercan, Frank Momburg, Ulrike Engel, Koen Temmerman, Walter Nickel and Matthias Seedorf (2009) A conserved, lipid-mediated mechanism for the sorting of yeast Ist2 and mammalian STIM proteins to plasma membrane-associated domains of the peripheral ER. Traffic 10:1802-1818

Lucía Cespón Torrado, Koen Temmerman, Hans-Michael Müller, Matthias P. Mayer, Claudia Seelenmeyer, Rafael Backhaus, and Walter Nickel (2009) An Intrinsic Quality Control Mechanism Ensures Unconventional Secretion of Fibroblast Growth Factor 2 in a Folded Conformation. J. Cell Sci. 122:3322-3329

Stella Tournaviti, Enrica San Pietro, Stefan Terjung, Tobias Schafmeier, Sabine Wegehingel, Julia Ritzerfeld, Juliane Schulz, Deborah F. Smith, Rainer Pepperkok, and Walter Nickel (2009) Reversible Phosphorylation as a Molecular Switch to Regulate Plasma Membrane Targeting of Acylated SH4 Domain Proteins. Traffic 10:1047-1060

Melanie Merk, John Baugh, Swen Zierow, Lin Leng, Utpal Pal, Seung Joon Lee, Antje D. Ebert, Yuka Mizue, John O. Trent, Robert Mitchell, Walter Nickel, Paula B. Kavathas, Jürgen Bernhagen, and Richard Bucala (2009) The Golgi-associated Protein p115 Mediates the Secretion of Macrophage Migration Inhibitory Factor. J. Immunol. 182:6896-6906

Marcel A. Fischer, Koen Temmerman, Walter Nickel and Matthias Seedorf (2009) A lipid-binding motif targets the yeast integral membrane protein Ist2 to domains of the cortical ER. Traffic 10:1084-1097

Koen Temmerman and Walter Nickel (2009) A novel flow cytometric assay to quantify interactions between proteins and membrane lipids. J. Lipid Res. 50:1245-1254

Kiran Maass, Marcel André Fischer, Markus Seiler, Koen Temmerman, Walter Nickel and Matthias Seedorf (2009) A signal comprising a basic cluster and an amphipathic α-helix interacts with lipids and is required for the transport of Ist2 to the yeast cortical ER. J. Cell Sci. 122:625-635

Walter Nickel and Catherine Rabouille (2009) Mechanisms of Regulated Unconventional Protein Secretion. Nat. Rev. Mol. Cell Biol. 10:148-155

2008

Walter Nickel and Matthias Seedorf (2008) Unconventional Mechanisms of Protein Transport to the Cell Surface of Eukaryotic Cells. Annu. Rev. Cell Dev. Biol. 24:287-308

Sabine Wegehingel, Christoph Zehe, and Walter Nickel (2008) Rerouting of Fibroblast Growth Factor 2 to the Classical Secretory Pathway Results in Post-translational Modifications That Block Binding to Heparan Sulfate Proteoglycans. FEBS Lett. 582:2387-2392

Koen Temmerman, Antje D. Ebert, Hans-Michael Müller, Irmgard Sinning, Ivo Tews, and Walter Nickel (2008) A Direct Role for Phosphatidylinositol-4,5-bisphosphate in Unconventional Secretion of Fibroblast Growth Factor 2. Traffic 9:1204-1217

Claudia Seelenmeyer, Carolin Stegmayer, and Walter Nickel (2008) Unconventional Secretion of Fibroblast Growth Factor 2 and Galectin-1 Does Not Require Shedding of Plasma-Membrane-Derived Vesicles. FEBS Lett. 582:1362-1368

2007

Walter Nickel (2007) Unconventional Protein Secretion: An Extracellular Trap For Nonclassical Export of Fibroblast Growth Factor 2. J. Cell Sci. 120:2295-2299

Tournaviti S, Hannemann S, Terjung S, Kitzing TM, Stegmayer C, Ritzerfeld J, Walther P, Grosse R, Nickel W, Fackler OT. SH4-domain-induced plasma membrane dynamization promotes bleb-associated cell motility.
J Cell Sci. 2007, 120 (Pt 21):3820-9.

2006

Christoph Zehe, André Engling, Sabine Wegehingel, Tobias Schäfer, and Walter Nickel (2006) Cell surface heparan sulfate proteoglycans are essential components of the unconventional export machinery of FGF-2. Proc. Natl. Acad Sci. U.S.A. 103:15479-15484

2005

Claudia Seelenmeyer, Sabine Wegehingel, Ivo Tews, Markus Künzler, Markus Aebi, and Walter Nickel (2005) Cell Surface Counter Receptors are Essential Components of the Unconventional Export Machinery of Galectin-1. J. Cell Biol. 172:373-381

Walter Nickel (2005) Unconventional Secretory Routes: Direct Protein Export Across the Plasma Membrane of Mammalian Cells. Traffic 6:607-614

Carolin Stegmayer, Angelika Kehlenbach, Stella Tournaviti, Sabine Wegehingel, Christoph Zehe, Paul W. Denny, Deborah F. Smith, Blanche Schwappach, and Walter Nickel (2005) Direct Transport Across the Plasma Membrane of Mammalian Cells of Leishmania HASPB as Revealed by a CHO Export Mutant. J. Cell Sci. 118:517-527

2004

Rafael Backhaus, Christoph Zehe, Sabine Wegehingel, Angelika Kehlenbach, Blanche Schwappach, and Walter Nickel (2004) Unconventional Protein Secretion: Membrane Translocation of FGF-2 Does Not Require Protein Unfolding. J. Cell Sci. 117:1727-1736

Tobias Schäfer, Hanswalter Zentgraf, Christoph Zehe, Britta Brügger, Jürgen Bernhagen, and Walter Nickel (2004) Unconventional Protein Secretion of Fibroblast Growth Factor 2 Is Mediated by Direct Translocation across the Plasma Membrane of Mammalian Cells. J. Biol. Chem. 279:6244-6251

2003

Oliver Flieger, André Engling, Richard Bucala, Hongqi Lue, Walter Nickel, and Jürgen Bernhagen (2003) Regulated secretion of macrophage migration inhibitory factor is mediated by a nonclassical pathway involving an ABC transporter. FEBS Lett. 551:78-86

Constanze Reinhard, Michael Schweikert, Felix T. Wieland, and Walter Nickel (2003) Functional reconstitution of COPI coat assembly and disassembly using chemically defined components. Proc. Natl. Acad Sci. U.S.A. 100:8253-8257

Walter Nickel (2003) The Mystery of Non-classical Protein Secretion: A Current View on Cargo Proteins and Potential Export Routes.
Eur. J. Biochem. 270:2109-2119

Claudia Seelenmeyer, Sabine Wegehingel, Johannes Lechner, and Walter Nickel (2003) The tumor antigen CA125 represents a novel counter receptor for galectin-1. J. Cell Sci. 116:1305-1318

2002

André Engling, Rafael Backhaus, Carolin Stegmayer, Christoph Zehe, Claudia Seelenmeyer, Angelika Kehlenbach, Blanche Schwappach, Sabine Wegehingel, and Walter Nickel (2002) Biosynthetic FGF-2 is Targeted to Non-Lipid Raft Microdomains Following Translocation to the Extracellular Surface of CHO cells. J. Cell Sci. 115:3619-3631

Walter Nickel, Britta Brügger, and Felix T. Wieland (2002) Vesicular Transport: the Core Machinery of COPI recruitment and budding. J. Cell Sci. 115:3235-3240 (Review)

2001

Daniel U.Gommel, Abdul R. Memon, Armin Heiss, Friedrich Lottspeich, Constanze Reinhard, J. Bernd Helms, Walter Nickel, and Felix T. Wieland (2001) Recruitment to Golgi Membranes of ADP-Ribosylation Factor 1 is Mediated by the Cytoplasmic Domain of p23. EMBO J. 20:6751-6760

Walter Nickel, Britta Brügger and Felix T. Wieland (2001) Membrane Trafficking: How Transport Vesicles Fuse With Target Membranes in "Protein Modules in Cellular Signalling", eds Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 257-261 (Review)

Britta Brügger, Walter Nickel, and Felix T. Wieland (2001) Molecular Mechanisms of COPI Vesicle Formation in "Protein Modules in Cellular Signalling", eds. Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 262-267 (Review)

Britta Brügger, Walter Nickel, and Felix T. Wieland (2001) Sorting Into COPI Vesicles of Protein and Lipid Cargo in "Protein Modules in Cellular Signalling", eds. Heilmeyer L. and Friedrich P. (IOS Press Amsterdam), pp. 268-275 (Review)

2000

Walter Nickel and Felix T. Wieland (2000) Receptor-Dependent Formation of COPI-Coated Vesicles From Chemically Defined Donor Liposomes. Methods Enzymol. 329:388-404

Britta Brügger, Roger Sandhoff, J.Bernd Helms, Karin Gorgas, Wolf D. Lehmann, Walter Nickel, F.T. Wieland (2000) Segregation from COPI-Coated Vesicles of Sphingomyelin and Cholesterol. J. Cell Biol. 151:507-518

Ryouichi Fukuda, James A. McNew, Thomas Weber, Francesco Parlati, Thomas Engel, Walter Nickel, James E. Rothman, and Thomas H. Söllner (2000) Functional Architecture of Intracellular t-SNAREs: Membrane Fusion by Isolated SNAREs of the Yeast Vacuole. Nature 407:198-202

Britta Brügger, Walter Nickel, Thomas Weber, Frank Parlati, James A. McNew, James E. Rothman, and Thomas H. Söllner (2000) Putative Fusogenic Activity of NSF is Restricted to Lipid Mixtures Whose Coalescence is Also Triggered by Other Factors. EMBO J. 19:1272-1278

Werner Zwerschke, Boris Mannhardt, Paola Massimi, Sonja Nauenburg, David Pim, Walter Nickel, Lawrence Banks, Arnold J. Reuser, Pidder Jansen-Dürr (2000) Allosteric activation of acid a-glucosidase by the human papillomavirus E7 protein. J. Biol. Chem. 275:9534-9541

1999

Jörg Malsam, Daniel Gommel, Felix. T. Wieland, and Walter Nickel (1999) A role for ADP-ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis. FEBS Lett. 462:267-272.

Walter Nickel, Thomas Weber, James A. McNew, Francesco Parlati, Thomas H. Söllner, and James E. Rothman (1999). Content mixing and membrane integrity during membrane fusion driven by pairing of isolated v-SNAREs and t-SNAREs. Proc. Natl. Acad Sci. U.S.A. 96 :12571-12576.

D. Gommel, L. Orci, E.M. Emig, M.J. Hannah, M. Ravazzola, W. Nickel, J.B. Helms, F.T. Wieland and K. Sohn (1999) p24 and p23, the major transmem-brane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. FEBS Lett. 447:179-185.

Walter Nickel and Britta Brügger (1999) COPI-Mediated Protein and Lipid Sorting. Protoplasma 207:115-124 (Review)

Martina Bremser*, Walter Nickel*, Michael Schweikert, Mariella Ravazzola, Mylène Amherdt, Christine A. Hughes, Thomas H. Söllner, James E. Rothman, and Felix T. Wieland (1999) Coupling of Coat Assembly and Vesicle Budding to Packaging of Putative Cargo Receptors. Cell 96:495-506 *first authors

1998

Walter Nickel, Britta Brügger, and Felix T. Wieland (1998) Protein and Lipid Sorting Between the Endoplasmic Reticulum and the Golgi Complex. Sem. Cell Dev. Biol. 9:493-501. (Review)

Walter Nickel, Jörg Malsam, Karin Gorgas, Mariella Ravazzola, Nicole Jenne, J. Bernd Helms, and Felix T. Wieland (1998) Uptake by COPI-coated Vesicles of Both Anterograde and Retrograde Cargo Molecules is Inhibited by GTPgS In Vitro. J. Cell Sci. 111:3081-3090.

J. Bernd Helms, Desire Helms-Brons, Britta Brügger, Ioannis Gkantiragas, Heike Eberle, Walter Nickel, Bernd Nürnberg, Hans-Hermann Gerdes, and Felix T. Wieland (1998) A Putative Heterotrimeric G Protein Inhibits the Fusion of COPI-coated Vesicles. J. Biol. Chem. 273:15203-15208.

Walter Nickel, Britta Brügger, and Felix T. Wieland (1998) Intracellular Transport of Glycoproteins and Glycolipids. Trends Glycosci. Glycotechnolog. 10:1-11 (Review)

Walter Nickel and Felix T. Wieland (1998) Biosynthetic Protein Transport Through the Early Secretory Pathway. Histochem. Cell Biol. 109:477-486. (Review)

1997

Walter Nickel and Felix T. Wieland (1997) Biogenesis of COPI-Coated Transport Vesicles. FEBS Lett. 413:395-400 (Review)

Walter Nickel, Kai Sohn, Carsten Bünning, and Felix T. Wieland (1997) p23, a major COPI vesicle membrane protein, constitutively cycles through the early secretory pathway. Proc. Natl. Acad. Sci. U.S.A. 94:11393-11398.

Walter Nickel and Klaus Leitzgen (1997) Protein-Reifung: Transport, Faltung und Abbau. Biospektrum 4: 22-24 (Review)

1996

Walter Nickel, J. Bernd Helms, Richard E. Kneusel, and Felix T. Wieland (1996) Forskolin stimulates detoxification of brefeldin A. J. Biol. Chem. 271:15870-15873.

1995

Andreas Barthel, Walter Nickel, Christiane Tonko, and Hans-Dieter Söling (1995) Sorting and budding of constitutive secretory vesicles in hepatocytes and hepatoma cells. Adv. Enzyme Regul. 35:283-292

1994

Walter Nickel, Richard A. Kahn, Nicola Kipper, Andreas Barthel, Dirk Faßhauer, and Hans-Dieter Söling (1994) ARF and VAPP14: Two Proteins Involved in the Delivery of Heparan Sulfate Proteoglycan From the TGN to the Cell Surface. Ann. NY Acad. Sci. 1994:344-356.

Walter Nickel, Lukas A. Huber, Richard A. Kahn, Nicola Kipper, Andreas Barthel, Dirk Faßhauer, and Hans-Dieter Söling (1994) ADP-ribosylation factor (ARF) and a 14 kDa Polypeptide Are Associated With Heparan Sulfate Proteoglycan-carrying Post-trans-Golgi network Secretory Vesicles. J. Cell Biol. 125:721-731.

Walter Nickel (1994) Funktionelle und strukturelle Untersuchungen zum vesikulären Golgi-Plasmamembran-Transport in Leberzellen. PhD thesis. Georg-August-Universität Göttingen. ©Cuvillier-Verlag Göttingen

1992

Walter Nickel, Sabine Austermann, Gabriele Bialek, and Frank Grosse (1992) Interactions of Azidothymidine Triphosphate with the Cellular DNA Polymerases alpha, delta and epsilon and with DNA Primase. J. Biol. Chem. 267:848-854  
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