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HEIDELBERG UNIVERSITY

Irmgard Sinning 
Molecular machines in protein targeting and membrane protein biogenesis

Groupleader: Irmgard Sinning


2017

Dodonova, S.O., Aderhold, P., Kopp, J., Ganeva, I., Röhling, S., Hagen, W.J.H., Sinning, I., Wieland, F., & Briggs, J.A.G. (2017) 9Å structure of COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments, Elife 6. pii: e26691.

Calviño, F.R., Kornprobst, M., Schermann, G., Birkle, F., Wild, K., Fischer, T., Hurt, E., Ahmed, Y.L. & Sinning, I. (2017) Structural basis for 5’-ETS recognition by Utp4 at the earliest step of ribosome biogenesis, PlosOne 12: e0178752.

Feilen, L.P., Haubrich, K., Strecker, P., Probst, S., Eggert, S., Stier, G., Sinning, I., Konietzko, U., Kins, S., Simon, B. & Wild, K. (2017) Fe65-PTB2 dimerization mimics Fe65-APP interaction, Front. Mol Neurosci. 10: 140.

Weyer, F.A., Gumiero, A., Lapouge, K., Bange, G., Kopp, J. & Sinning, I. (2017) Structural basis of HypK regulating N-terminal acetylation by the NatA complex, Nat Comms 8: 15726.

Rodrigues, M.J., Windeisen, V., Zhang,Y., Guédez,G., Weber, S., Strohmeier, M., Hanes, J.W., Royant, A., Evans, G., Sinning, I., Ealick, S.E., Begley, T.P. & Tews, I. (2017) Lysine relay mechanism coordinates intermediate transfer in vitamin B6 biosynthesis, Nat. Chem. Biol. 3: 290-294.

Korytowski, A., Abuillan, W., Amadei, F., Makky, A., Gumiero, A., Sinning, I., Gauss, A., Stremmel, W. & Tanaka, M. (2017) Accumulation of phosphatidylcholine on gut intestinal mucosa surface is not dominated by electrostatic interactions, BBA Biomembranes 1859: 959-965.

Weyer, F. A., Gumiero, A., Valentín Gesé, G., Lapouge, K. & Sinning, I. (2017) Structural insights into a unique Hsp70/Hsp40 interaction in the eukaryotic ribosome-associated complex, Nat. Struct. Mol. Biol. 24:144-151. F1000Prime recommended

Becker, M.M.M., Lapouge, K., Segnitz, B., Wild, K. & Sinning, I. (2017) Structures of human SRP72 reveal its function in co-translational protein targeting, Nucl. Acids Res. 45: 470-481.

Baßler, J., Ahmed, Y.L., Kallas, M., Kornprobst, M., Rodriguez-Calvino, F., Gnädig, M., Thoms, M., Stier, G., Ismail, S., Kharde, S., Castillo, N., Griesel, S., Bastuck, S., Bradatsch, B., Thomson, E., Flemming, D., Sinning, I.* & Hurt, E.* (2017) Interaction network of the ribosome assembly machinery from a eukaryotic thermophile, Protein Science 26: 327-342.

2016

Ohle, C., Tesorero, R., Schermann, G., Dobrev, N., Sinning, I. & Fischer, T. (2016) RNA-DNA hybrids and RNase H activity are required for efficient DSB repair, Cell 167: 1001-13.

Gumiero, A., Conz, C., Valentín Gesé, G., Zhang, Y., Weyer, F. A., Lapouge, K., Kappes, J., von Plehwe, U., Schermann, G., Fitzke, E., Wölfle, T., Fischer, T., Rospert, S. & Sinning, I. (2016) Interaction of the cotranslational Hsp70 Ssb with ribosomal proteins and rRNA depends on its lid domain, Nat. Comms. 7: 13563.

Wild, K., Bange, G., Motiejunas, D., Kribelbauer, J., Hendricks, A., Segnitz, B., Wade, R., & Sinning, I. (2016) Structural basis for conserved regulation and adaptation of the signal recognition particle targeting complex, J. Mol. Biol. 428: 2880-97.

Thoms, M., Ahmed. Y.L., Maddi, K., Hurt, E. & Sinning, I. (2016) Concerted removal of the Erb1-Ytm1 complex in ribosome biogenesis relies on an elaborate interface, Nucl. Acids Res. 44: 926-39.

2015

Horn, A., Hennig, J., Ahmed, J.L., Stier, G., Wild, K., Sattler, M. & Sinning, I. (2015) Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction, Nat Comms. 6: 8875.

Jadhav, B., McKenna, M., Johnson, N., High, S., Sinning, I. & Pool, M.R. (2015) Mammalian SRP receptor switches the Sec61 translocase from Sec62 to SRP-dependent translocation, Nat Comms. 6: 10133.

Beckert, B., Kedrov, A., Sohmen, D., Kempf, G., Wild, K., Sinning, I., Stahlberg, H., Wilson, D. & Beckmann, R. (2015) Translational arrest by a prokaryotic signal recognition particle is mediated by RNA interactions, Nat. Struct. Mol. Biol. 22: 767-73.

Jadhav, B., Wild, K., Pool, M.R. & Sinning, I. (2015) Structure and switch cycle of SRβ as ancestral eukaryotic GTPase associated with secretory membranes, Structure 23: 1838-47.

Bassler, J., Paternoga, H., Holdermann, I., Thoms, M., Grannemann, S., Barrio-Garcia, C., Nyarko, A., Lee, W. Stier, G., Clark, S., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning*, I. & Hurt*, E. (2015) A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation, J. Cell Biol. 210: 169-70.

Kharde, S., Calviño, F.R., Gumiero, A., Wild, K. & Sinning, I. (2015) The structure of Rpf2-Rrs1 explains its role in ribosome biogenesis, Nucl. Acids Res. 43: 7083-95.

Pausch, P., Singh, U., Ahmed, Y.L., Pillet, B., Altegoer, F., Stier, G., Thoms, M., Hurt, E., Sinning, I., Bange, G. & Kressler, D. (2015) Co-translational capturing of nascent ribosomal proteins by their dedicated chaperones, Nat. Comms 6:7494.

Kempf, G., Wild, K. & Sinning, I. (2015) Strukturelle Evolution des Signalerkennungspartikels, Biospektrum 02: 161-163.

Calviño, F.R., Kharde, S., Ori, A., Hendricks, A., Wild, K., Kressler, D., Bange, G., Hurt, E., Beck, M. & Sinning, I. (2015) Syo1 chaperones 5S-RNP assembly during ribosome biogenesis by RNA mimicry, Nat. Commun. 6: 6510.

Hackmann, Y., Jödicke, L., Panneels, V. & Sinning, I. (2015) Methods Enzymol. 556: 219-39.

Müller, H.M., Steringer, J.S., Wegehingel, S., Bleicken, S., Münster, M., Dimou, E., Unger, S., Weidmann, G., Andreas H., Garcia-Saez, A., Wild, K., Sinning, I. & Nickel, W. (2015) Formation of Disulfide Bridges Drives oligomerization, membrane pore formation and translocation of fibroblast growth factor 2 to cell surfaces, J. Biol. Chem. 290: 8925-37.

2014

Wild, K. & Sinning, I. (2014) RNA gymnastics in mammalian signal recognition particle assembly, RNA Biol. 11: 1330-1334.

Kempf, G., Wild, K. & Sinning, I. (2014) Structure of the tRNA-like bacterial Alu domain Nucl. Acids Res. 42: 12284-94.

Grotwinkel, J.T., Wild, K., Segnitz, B. & Sinning, I. (2014) SRP RNA remodeling by SRP68 explains its role in protein translocation, Science 344: 101-104. DOI:10.1126/science.1249094.

Bassler, J., Paternoga, H., Holdermann, I., Thoms, M., Grannemann, S., Barrio-Garcia, C., Nyarko, A., Stier, G., Clark, S., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning*, I. & Hurt*, E. (2014) A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation, J. Cell Biol. 207: 481-498.

Leidig, C., Thoms, M., Holdermann, I., Bradatsch, B., Berninghausen, O., Bange, G., Sinning, I., Hurt, E. & Beckmann, R. (2014) 60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle, Nat. Commun. 5: 3491. doi: 10.1038/ncomms4491.

Parisotto, D., Pfau, M., Scheutzow, A., Wild, K., Mayer, M.P., Malsam, J., Sinning, I. & Söllner, T.H. (2014) An extended helical conformation in Munc18-1 domain 3a provides a template for SNAREpin assembly, J. Biol. Chem., 2014 Feb 14. [Epub ahead of print] PMID: 24532794.

De Franceschi, N., Wild, K., Schlacht, A., Dacks, J.B., Sinning, I. & Filippini, F. (2014) Longin and GAF Domains: Structural Evolution and Adaptation to the Subcellular Trafficking Machinery. Traffic 15: 104-21.

2013

Denic, V., Doetsch, V. & Sinning, I. (2013) Endoplasmic reticulum Targeting and Insertion of Tail-anchored membrane proteins by the GET pathway. Cold Spring Harb Perspect Biol. 5(8). doi:pii: a013334.

Bange, G. & Sinning, I. (2013) SIMIBI Twins in protein targeting and localization, Nat. Struct. Mol. Biol. 20:776-80. doi: 10.1038/nsmb.2605.

Bange, G. , Murat, G., Sinning, I., Hurt, E. & Kressler, D. (2013) New twist to nuclear import: When two travel together, Com. & Int. Biol. 6:4, e24792; July/August 2013, Landes Bioscience).

van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S., Bange, G., Creevey, C., Falk, S., Mende, D.R., Sinning, I., Hurt, E. & Bork, P. (2012) Consistent mutational paths predict eukaryotic thermostability, BMC Evol Biol 13: 7.

Leidig, C., Bange, G., Kopp, J., Amlacher, S., Aravind, A., Wickles, S., Witte, G., Hurt, E., Beckmann, R. & Sinning, I. (2013) Structural characterization of a eukaryotic co-translational chaperone, the ribosome-associated complex (RAC), Nat. Struct. Mol. Biol. 20:  23-8.

2012

Kityk, R., Kopp, J., Sinning, I. & Mayer, M.P. (2012) Structure and dynamics of the ATP-bound open conformation of Hsp70 chaperones, Mol Cell, 2012 Nov 1. doi:pii: S1097-2765(12)00823-4. 10.1016/j.molcel.2012.09.023.

Kressler*, D., Bange, G. Ogawa, Y., Bradatsch, B., Pratte, D., Strauß, D., Amlacher, S., Yoneda,Y., Katahira, J., Sinning, I.*, & Hurt, E.* (2012) Synchronizing nuclear import of ribosomal proteins with ribosome assembly, Science 338: 666-71.

Tripp, J., Hahn, A., Koenig, P., Flinner, N., Bublak, D., Ertel, F., Brouwer, E.M., Mirus, O., Sinning, I., Tews, I. & Schleiff, E. (2012) Structure and conservation of the periplasmic targeting factor tic22 protein from plants and cyanobacteria, J. Biol. Chem. 287: 24164-73.

Guédez, G., Hipp, K., Windeisen, V., Derrer, B., Gengenbacher, M., Böttcher, B., Sinning, I., Kappes, B. & Tews, I. (2012) Assembly of the eukaryotic PLP-synthase complex from Plasmodium and activation of the Pdx1 enzyme, Structure 20:172-84.

Holdermann, I., Meyer, H., Round, A., Wild, K., Sattler, M. & Sinning, I. (2012) Chromodomains read the arginine code of post-translational targeting, Nat. Struct. Mol. Biol. 19: 260-3.

2011

Guédez, G., Hipp, K., Windeisen, V., Derrer, B., Gengenbacher, M., Böttcher, B., Sinning, I., Kappes, B. & Tews, I. Assembly of the eukaryotic PLP-synthase complex from Plasmodium and activation of the Pdx1 enzyme, Structure. 2011, in press.

Klug W, Dietl A, Simon B, Sinning I, Wild K. Phosphorylation of LRP1 regulates the interaction with Fe65. FEBS Lett. 2011;585: 3229-35.

Sinning, I., Bange, G. & Wild, K. It takes two to Get3. Structure. 2011;19:1353-55.

Bange G, Kümmerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I. Structural basis for the molecular evolution of SRP-GTPase activation by protein. Nat Struct Mol Biol. 2011;8:1376-80.

Pietrosiuk A, Lenherr ED, Falk S, Bönemann G, Kopp J, Zentgraf H, Sinning I, Mogk A. Molecular Basis for the Unique Role of the AAA+ Chaperone ClpV in Type VI Protein Secretion. J Biol Chem. 2011;286(34):30010-21.

Stefer S, Reitz S, Wang F, Wild K, Pang YY, Schwarz D, Bomke J, Hein C, Löhr F, Bernhard F, Denic V, Dötsch V, Sinning I. Structural basis for tail-anchored membrane protein biogenesis by the Get3-receptor complex. Science. 2011;333(6043):758-62.

Lupo D, Vollmer C, Deckers M, Mick DU, Tews I, Sinning I, Rehling P. Mdm38 is a 14-3-3-Like Receptor and Associates with the Protein Synthesis Machinery at the Inner Mitochondrial Membrane. Traffic. 2011;12 (10):1457-66.

Stjepanovic G, Kapp K, Bange G, Graf C, Parlitz R, Wild K, Mayer MP, Sinning I. Lipids trigger a conformational switch that regulates signal recognition particle (SRP)-mediated protein targeting. J Biol Chem. 2011;286(26):23489-97. Epub 2011 May 3.

Panneels V, Kock I, Krijnse-Locker J, Rezgaoui M, Sinning I. Drosophila photoreceptor cells exploited for the production of eukaryotic membrane proteins: receptors, transporters and channels. PLoS One. 2011;6(4):e18478.

Cain P, Holdermann I, Sinning I, Johnson AE, Robinson C. Binding of chloroplast signal recognition particle to a thylakoid membrane protein substrate in aqueous solution and delineation of the cpSRP43-substrate interaction domain. Biochem J. 2011;437(1):149-55.

Oreb M, Höfle A, Koenig P, Sommer MS, Sinning I, Wang F, Tews I, Schnell DJ, Schleiff E. Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33. Biochem J. 2011; 436(2):313-9.

2010

Falk S, Sinning I. The C terminus of Alb3 interacts with the chromodomains 2 and 3 of cpSRP43.
J Biol Chem. 2010; 285(53):le25-6;

Erez E, Stjepanovic G, Zelazny AM, Brügger B, Sinning I, Bibi E. Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo.
J Biol Chem. 2010; 285(52):40508-14.

Derrer B, Windeisen V, Guédez Rodríguez G, Seidler J, Gengenbacher M, Lehmann WD, Rippe K, Sinning I, Tews I, Kappes B. Defining the structural requirements for ribose 5-phosphate-binding and intersubunit cross-talk of the malarial pyridoxal 5-phosphate synthase.
FEBS Lett. 2010; 584(19):4169-74.

Bange G, Kümmerer N, Engel C, Bozkurt G, Wild K, Sinning I. FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system.
Proc Natl Acad Sci U S A. 2010; 107(25):11295-300.

Falk S, Sinning I. cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins.
J Biol Chem. 2010; 285(28):21655-61.

Schott A, Ravaud S, Keller S, Radzimanowski J, Viotti C, Hillmer S, Sinning I, Strahl S. Arabidopsis stromal-derived Factor2 (SDF2) is a crucial target of the unfolded protein response in the endoplasmic reticulum.
J Biol Chem. 2010; 285(23):18113-21.

König P, Mirus O, Haarmann R, Sommer MS, Sinning I, Schleiff E, Tews I. Conserved properties of polypeptide transport-associated (POTRA) domains derived from cyanobacterial Omp85.
J Biol Chem. 2010; 285(23):18016-24.

Bozkurt G, Wild K, Amlacher S, Hurt E, Dobberstein B, Sinning I. The structure of Get4 reveals an alpha-solenoid fold adapted for multiple interactions in tail-anchored protein biogenesis.
FEBS Lett. 2010; 584(8):1509-14.

Schrul B, Kapp K, Sinning I, Dobberstein B. Signal peptide peptidase (SPP) assembles with substrates and misfolded membrane proteins into distinct oligomeric complexes.
Biochem J. 2010; 427(3):523-34.

Wild K, Bange G, Bozkurt G, Segnitz B, Hendricks A, Sinning I. Structural insights into the assembly of the human and archaeal signal recognition particles.
Acta Crystallogr D Biol Crystallogr. 2010; 66(Pt 3):295-303.

Panneels V, Sinning I. Membrane protein expression in the eyes of transgenic flies.
Methods Mol Biol. 2010; 601:135-47.

Radzimanowski J, Ravaud S, Schott A, Strahl S, Sinning I. Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of SDF2-like protein from Arabidopsis thaliana.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010; 66(Pt 1):12-4.

Falk S, Ravaud S, Koch J, Sinning I. The C terminus of the Alb3 membrane insertase recruits cpSRP43 to the thylakoid membrane.
J Biol Chem. 2010; 285(8):5954-62.

2009

Bozkurt G, Stjepanovic G, Vilardi F, Amlacher S, Wild K, Bange G, Favaloro V, Rippe K, Hurt E, Dobberstein B, Sinning I. Structural insights into tail-anchored protein binding and membrane insertion by Get3.
Proc Natl Acad Sci U S A. 2009; 106(50):21131-6.

Kock I, Bulgakova NA, Knust E, Sinning I, Panneels V. Targeting of Drosophila rhodopsin requires helix 8 but not the distal C-terminus.
PLoS One. 2009; 4(7):e6101.

Grudnik P, Bange G, Sinning I. Protein targeting by the signal recognition particle.
Biol Chem. 2009; 390(8):775-82. Review.

Neuwirth M, Strohmeier M, Windeisen V, Wallner S, Deller S, Rippe K, Sinning I, Macheroux P, Tews I. X-ray crystal structure of Saccharomyces cerevisiae Pdx1 provides insights into the oligomeric nature of PLP synthases.
FEBS Lett. 2009; 583(13):2179-86.

Cross BC, Sinning I, Luirink J, High S. Delivering proteins for export from the cytosol.
Nat Rev Mol Cell Biol. 2009; 10(4):255-64. Review.

Sinning I, Wild K, Bange G. Signal sequences get active.
Nat Chem Biol. 2009; 5(3):146-7.

Wallner S, Neuwirth M, Flicker K, Tews I, Macheroux P. Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis.
Biochemistry. 2009; 48(9):1928-35.

2008

Bionda T, König P, Oreb M, Tews I, Schleiff E. pH Sensitivity of the GTPase Toc33 as a regulatory circuit for protein translocation into chloroplasts.
Plant Cell Physiol. 2008; 49(12):1917-21.

Oreb M, Tews I, Schleiff E. Policing Tic 'n' Toc, the doorway to chloroplasts.
Trends Cell Biol. 2008; 18(1):19-27. Review.

Radzimanowski J, Simon B, Sattler M, Beyreuther K, Sinning I, Wild K. Structure of the intracellular domain of the amyloid precursor protein in complex with Fe65-PTB2.
EMBO Rep. 2008; 9(11):1134-40.

Beck R, Sun Z, Adolf F, Rutz C, Bassler J, Wild K, Sinning I, Hurt E, Brügger B, Béthune J, Wieland F. Membrane curvature induced by Arf1-GTP is essential for vesicle formation.
Proc Natl Acad Sci U S A. 2008; 105(33):11731-6.

Melchers J, Diechtierow M, Fehér K, Sinning I, Tews I, Krauth-Siegel RL, Muhle-Goll C. Structural basis for a distinct catalytic mechanism in Trypanosoma brucei tryparedoxin peroxidase.
J Biol Chem. 2008; 283(44):30401-11.

Stengel KF, Holdermann I, Cain P, Robinson C, Wild K, Sinning I. Structural basis for specific substrate recognition by the chloroplast signal recognition particle protein cpSRP43.
Science. 2008; 321(5886):253-6.

Radzimanowski J, Ravaud S, Schlesinger S, Koch J, Beyreuther K, Sinning I, Wild K. Crystal structure of the human Fe65-PTB1 domain.
J Biol Chem. 2008; 283(34):23113-20.

König P, Oreb M, Rippe K, Muhle-Goll C, Sinning I, Schleiff E, Tews I. On the significance of Toc-GTPase homodimers.
J Biol Chem. 2008; 283(34):23104-12.

Radzimanowski J, Ravaud S, Beyreuther K, Sinning I, Wild K. Mercury-induced crystallization and SAD phasing of the human Fe65-PTB1 domain.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008; 64(Pt 5):382-5.

Radzimanowski J, Beyreuther K, Sinning I, Wild K. Overproduction, purification, crystallization and preliminary X-ray analysis of human Fe65-PTB2 in complex with the amyloid precursor protein intracellular domain.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008; 64(Pt 5):409-12.

Temmerman K, Ebert AD, Müller HM, Sinning I, Tews I, Nickel W. A direct role for phosphatidylinositol-4,5-bisphosphate in unconventional secretion of fibroblast growth factor 2.
Traffic. 2008; 9(7):1204-17.

Ravaud S, Stjepanovic G, Wild K, Sinning I. The crystal structure of the periplasmic domain of the Escherichia coli membrane protein insertase YidC contains a substrate binding cleft.
J Biol Chem. 2008; 283(14):9350-8.

König P, Oreb M, Höfle A, Kaltofen S, Rippe K, Sinning I, Schleiff E, Tews I. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures.
Structure. 2008; 16(4):585-96.

Ravaud S, Wild K, Sinning I. Purification, crystallization and preliminary structural characterization of the periplasmic domain P1 of the Escherichia coli membrane-protein insertase YidC.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008; 64(Pt 2):144-8.

2007
Stengel KF, Holdermann I, Wild K, Sinning I. The structure of the chloroplast signal recognition particle (SRP) receptor reveals mechanistic details of SRP GTPase activation and a conserved membrane targeting site.
FEBS Letters 2007, 581:5671-6.

Bange G, Wild K, Sinning I. Protein translocation: Checkpoint role for SRP GTPase activation.
Curr. Biol. 2007, 17:R980-R982.

Parlitz R, Eitan A, Stjepanovic G, Bahari L, Bange G, Bibi E, Sinning I. E. coli SRP-receptor FtsY contains an essential and autonomous membrane-binding amphipathic helix.
J. Biol. Chem. 2007, 282:32176-184.

Bahari L, Parlitz R, Eitan A, Stjepanovic G, Bochkareva ES, Sinning I, Bibi E. Membrane targeting of ribosomes and their release require distinct and separable functions of FtsY.
J. Biol. Chem. 2007, 282:32168-175.

Kowalinski E, Bange G, Bradatsch B, Hurt E, Wild K, Sinning I. The crystal structure of Ebp1 reveals a methionine aminopeptidase fold as binding platform for multiple interactions.
FEBS Letters 2007, 581: 4450-4454.

Bradatsch B, Katahira J, Kowalinski E, Bange G, Yao W, Sekimoto T, Baumgärtel V, Boese G, Bassler J, Wild K, Peters R, Yoneda Y, Sinning I, Hurt E. Arx1 functions as an unorthodox nuclear export receptor for the 60S pre-ribosomal subunit,
Molecular Cell 2007, 27:767-79.

Kowalinski E, Bange G, Wild K, Sinning I. Expression, purification, crystallization and preliminary crystallographic analysis of the proliferation associated protein Ebp1.
Acta Cryst F 2007, 63: 768-770.

Bange G, Petzold G, Wild K, Parlitz RO, Sinning I. The crystal structure of the third SRP GTPase FlhF reveals a homodimer with bound GTP.
Proc. Natl. Acad.Sci. USA 2007, 104: 13621-13625.

Bange G, Petzold G, Wild K, Sinning I. Expression, purification and preliminary crystallographic characterization of FlhF from Bacillus subtilis,
Acta Cryst F. 2007, 63: 449-451.

Findeisen F, Linder JU, Schultz A, Schultz JE, Brügger B, Wieland F, Sinning I, Tews I. The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid.
J. Mol. Biol. 2007, 369:1282-95.

Tzvetkova-Chevolleau T, Hutin C, Noël LD, Goforth R, Carde JP, Caffarri S, Sinning I, Groves M, Teulon JM, Hoffman NE, Henry R, Havaux M, Nussaume L. Canonical Signal Recognition Particle Components Can Be Bypassed for Posttranslational Protein Targeting in Chloroplasts.
Plant Cell 2007,19:1635-48.

Peplowska K, Markgraf DF, Ostrowicz CW, Bange G, Ungermann C. The CORVET tethering complex interacts with the yeast Rab5 homolog Vps21 and is involved in endo-lysosomal biogenesis.
Dev Cell. 2007, 12:739-50.

Flicker K, Neuwirth M, Strohmeier M, Kappes B, Tews I, Macheroux P. Structural and Thermodynamic Insights into the Assembly of the Heteromeric Pyridoxal Phosphate Synthase from Plasmodium falciparum.
J Mol Biol. 2007, 374:732-48.

Fitzpatrick TB, Amrhein N, Kappes B, Macheroux P, Tews I, Raschle T. Two independent routes of de novo vitamin B6 biosynthesis: not that different after all. Biochem J. 2007, 407:1-13.

Tambasco-Studart M, Tews I, Amrhein N, Fitzpatrick TB. Functional analysis of PDX2 from Arabidopsis, a glutaminase involved in vitamin B6 biosynthesis.
Plant Physiol. 2007, 144:915-25.

Neuwirth M, Flicker K, Strohmeier M, Tews I, Macheroux P. Thermodynamic characterization of the protein-protein interaction in the heteromeric Bacillus subtilis pyridoxalphosphate synthase.
Biochemistry 2007, 46:5131-9.

2006

Motaal A, Tews I, Schultz JE, Linder JU. Fatty acid regulation of adenylyl cyclase Rv2212 from Mycobacterium tuberculosis H37Rv.
FEBS J. 2006, 273(18):4219-28.

Qbadou S, Becker T, Mirus O, Tews I, Soll J, Schleiff E. The molecular chaperone Hsp90 delivers precursor proteins to the chloroplast import receptor Toc64.
EMBO J. 2006, 25(9):1836-47.

Gengenbacher M, Fitzpatrick TB, Raschle T, Flicker K, Sinning I, Müller S, Macheroux P, Tews I, Kappes B. Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: Biochemical and structural insights.
J Biol Chem. 2006, 281(6):3633-41.

Strohmeier M, Raschle T, Mazurkiewicz J, Rippe K, Sinning I, Fitzpatrick TB, Tews I. Structure of a bacterial pyridoxal 5'-phosphate synthase complex.
Proc Natl Acad Sci U S A. 2006, 103(51):19284-9.

Halic M, Blau M, Becker T, Mielke T, Pool MR, Wild K, Sinning I, Beckmann R. Following the signal sequence from ribosomal tunnel exit to signal recognition particle.
Nature. 2006, 444(7118):507-11.

Halic M, Gartmann M, Schlenker O, Mielke T, Pool MR, Sinning I, Beckmann R. Signal recognition particle receptor exposes the ribosomal translocon binding site.
Science. 2006, 312(5774):745-7.

Schlenker O, Hendricks A, Sinning I, Wild K. The structure of the mammalian signal recognition particle (SRP) receptor as prototype for the interaction of small GTPases with Longin domains.
J Biol Chem. 2006, 281(13):8898-906.

2005

Tews, I., Findeisen, F., Sinning, I., Schultz A., Schultz, J.E. & Linder, J.U. (2005) The structure of a pH sensing mycobacterial adenylyl cyclase holoenzyme, Science 308: 1020-1023.

2004

Luirink, J. & Sinning, I. (2004) SRP-mediated protein targeting: structure and function revisited, BBA Mol. Cell Res. 1694: 17-35.

Wild, K., Halic, M., Sinning, I. & Beckmann, R. (2004) Co-translational protein targeting: SRP meets the ribosome, Nat. Struct. Mol. Biol. 11: 1049-53.

Abell. B., Pool., M., Schlenker, O., Sinning, I. & High, S. (2004) Signal recognition particle mediates posttranslational targeting in eukaryotes, EMBO J. 23:2755-64.

Wild, K., Rosendal, K. & Sinning, I. (2004) A structural step into the SRP cycle, Mol. Microbiol. 53: 357-63.

Serrano, R.L., Kuhn, A., Hendricks, A., Helms, J.B., Sinning, I. & Groves, M.R. (2004) Structural analysis of the human Golgi-associated plant pathogenesis related protein GAPR-1 implicates dimerization as a regulatory mechanism, J. Mol. Biol. 339: 173-83.

Groves, M.R., Kuhn, A., Hendricks, A., Radke, S., Serrano, R.L., Helms, J.B & Sinning, I. (2004) Crystallization of a Golgi-associated PR-1 related protein (GAPR-1) that localizes to lipid-enriched microdomains, Acta Cryst. D60: 730–732.

Dobberstein, B. & Sinning. I. (2004) Structural biology. Surprising news from the PCC. Science 303: 320-2.

Rosendal, K.R., Sinning, I. & Wild, K. (2004) Crystallization and preliminary X-ray diffraction studies of the crenarchaeal SRP core, Acta Cryst. D60: 140-143.

2003

Vasiljev, A., Ahting, U., Nargang, F.E., Go, N.E., Habib, S.J., Kozany, C., Panneels, V., Sinning, I., Prokisch, H., Neupert, W., Nussberger, S. & Rapaport, D. (2003) Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are sufficient for translocation of the ADP/ATP carrier across membranes, Mol. Biol. Cell 15: 1445-58.

Rosendal, K.R., Wild, K., Montoya, G. & Sinning, I. (2003) Crystal structure of the complete core of archaeal signal recognition particle and implications for inter-domain communication, Proc. Natl. Acad. Sci. USA 100: 14701-14706.

Eroglu, C., Bruegger, B., Wieland, F. & Sinning, I. (2003) Glutamate binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts. Proc. Natl. Acad. Sci. USA 100: 10219-10224.

Panneels, V., Schüssler, U., Costagliola, S. & Sinning, I. (2003) Choline head groups stabilize the matrix loop regions of the ATP/ADP carrier ScAAC2. Biochem. Biophys. Res. Com. 300: 65-74.

Sprangers, R., Selenko, P., Sattler, M., Sinning, I. & Groves, M.R. (2003) Definition of Domain Boundaries and Crystallization of the SMN Tudor Domain. Acta Cryst. D59: 366-368.

Sprangers, R., Groves, M.R., Sinning, I. & Sattler, M. (2003) High-resolution X-Ray and NMR structures of the SMN Tudor Domain: conformational variation in the binding site for symmetrically dimethylated arginines, J. Mol.Biol. 327: 507-520.

Panneels, V., Eroglu, C., Cronet, P. & Sinning, I. (2003) Pharmacological characterization and immunoaffinity purification of metabotropic glutamate receptor from Drosophila melanogaster overexpressed in SF9 cells, Prot. Exp. Pur. 30: 275-282.

2002

Groves, M., & Sinning, I. (2002) Chapter 6: Protein/Protein Interactions, in “Peptide arrays on membrane supports: Synthesis and applications” (J. Koch and M. Mahler, eds.) Springer Verlag Heidelberg, Germany, pp.83-96.

Wild, K., Weichenrieder, O., Strub, K., Sinning, I. & Cusack, S. (2002) Towards the structure of the mammalian signal recognition particle. Curr. Opin. Struct. Biol.12: 72–81.

Eroglu, C., Cronet, P., Panneels, V., Beaufils, P. & Sinning, I. (2002) Functional reconstitution of purified mGluR overexpressed in the eye of transgenic Drosophila melanogaster, EMBO Rep. 3: 491-496.

Pool, M.R., Stumm, J., Fulga, T.A., Sinning, I. & Dobberstein, B. (2002) Distinct modes of Signal Recognition Particle interaction with the Ribosome, Science 297: 1345-8

2001

Fulga, T., Sinning, I., Dobberstein, B. & Pool, M.R. (2001) SRbeta coordinates signal sequence release from SRP with ribosome binding to the translocon. EMBO J. 20: 2338-47.

Groves M., Mant A., Kuhn A., Koch J, Dubel S., Robinson C. & Sinning I. (2001) Functional characterization of recombinant chloroplast signal recognition particle. J. Biol. Chem. 276: 27778-86.

Wild, K., Sinning, I. & Cusack , S. (2001) Crystal structure of an early protein-RNA assembly complex of human signal recognition particle. Science 294: 598-601.

2000

de Leeuw, E., te Kaat, K., Moser, C., Menestrina, G., Demel, R., de Kruijff, B., Luirink, J. & Sinning, I. (2000) Anionic phospholipids are involved in membrane association of the Signal Recognition Particle receptor FtsY and stimulate its GTPase activity, EMBO J. 19: 531-541.

Brugna, M., Rodgers, S., Schricker, A., Montoya, G., Kazmeier, M., Nitschke, W. & Sinning, I. (2000) A spectroscopic method for observing the domain movement of the Rieske iron sulphur protein, Proc. Natl. Acad. Sci. USA 97: 2069-2074.

Montoya, G., te Kaat, K., Moll, R., Schäfer, G. & Sinning, I. (2000) The crystal structure of the conserved GTPase of SRP54 from the archaeon Acidianus ambivalens and its comparison with related structures suggests a model for the SRP/SR receptor complex, Struct. Fold. Des. 8: 515-525.

Rodgers, S., Moser, C., Martinez-Julvez, M. & Sinning, I. (2000) Deletion of the 6-kDa subunit affects the activity and yield of the bc1 complex from Rhodovulum sulfidophilum. Eur. J. Biochem. 267: 3753-61.

before 2000

Montoya, G., te Kaat, K., Rodgers, S., Nitschke, W. & Sinning, I. (1999) The cytochrome bc1 complex from Rhodovulum sulfidophilum is a dimer with six quinones per monomer and an additional 6-kDa component, Eur. J. Biochem. 259: 709-718.

Montoya, G., te Kaat, K., Moll, R., Schäfer, G. & Sinning, I. (1999) Crystallization and preliminary X-ray diffraction studies on the conserved GTPase domain of the signal recognition particle from Acidianus ambivalens, Acta Cryst. D55: 1949-1951.

Montoya, G., Svensson, C., Savage, H., Schwenn, J.D. & Sinning, I. (1998) Crystallization and preliminary X-ray diffraction study of phospho-adenylylsulphate (PAPS) reductase from E.coli, Acta Cryst. D54: 281-283.

Montoya, G., Svensson, C., Luirink, J. & Sinning, I. (1997) Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle, Proteins 28: 285-288.

Montoya, G., Svensson, C., Luirink, J. & Sinning, I. (1997) Crystal structure of the NG-domain of the signal-recognition particle receptor FtsY, Nature 385: 365-368.

Savage, H., Montoya, G., Svensson, C., Schwenn, J.D. & Sinning, I. (1997) Crystal structure of phospho-adenylyl-sulfate (PAPS) reductase: a new family of adenine nucleotide  hydrolases, Structure 5: 895-906.

Moser, C., Mol, O., Goody, R. & Sinning, I. (1997) The SRP receptor of E. coli has a nucleotide exchange factor built in to the GTPase domain, Proc. Natl. Acad. Sci. USA 94: 11339-11344.

Kleywegt, G.J., Zou, J.-Y., Divne, C., Davies, G.J., Sinning, I., Stahlberg, J., Reinikainen, T., Srisodsuk, M., Teeri, T.T. & Jones, T.A. (1997) The crystal structure of the catalytic core domain of endoglucanaseI from Trichoderma resei at 3.6Å resolution, and a comparison with related enzymes. J. Mol. Biol. 272: 383-397.

de Leeuw, E., Poland, D., Mol, O., Sinning, I., ten Hagen-Jongman, C., Oudega, B., Luirink, J. (1997) Membrane association of FtsY, the E. coli SRP receptor. FEBS Lett. 416: 225-229.

Savage, H., Cyrklaff M., Montoya, G., Kühlbrandt, W. & Sinning, I. (1996) Two-dimensional structure of light-harvesting complex II (LHII) from the purple bacterium Rhodovulum sulfidophilum and comparison with LHII from Rhodopseudomonas acidophila, Structure 4: 243-252.

Deisenhofer, J., Epp, O., Sinning, I. & Michel, H., Crystallographic refinement at 2.3 A resolution and refined model of the photosynthetic reaction centre from Rhodopseudomonas viridis (1995) J. Mol. Biol. 246: 429-457.

Björnestedt, R., Stenberg, G., Widersten, M., Board, P., Sinning, I., Jones, T.A. & Mannervik, B. Functional significance of arginine 15 in the active site of human class Alpha glutathione transferase A1-1 (1995) J. Mol. Biol. 247: 765-773.

Cameron, A. D., Sinning, I., l’Hermite, G., Olin, B., Board, P.G., Mannervik, B. & Jones T.A., Structural analysis of human alpha-class glutathione transferase A1-1 in the apo-form and in comple with ethycrynic acid and its glutathione conjugate (1995) Structure 3: 717-727.

Montoya, G., Cyrklaff, M. & Sinning, I. (1995) Two-dimensional crystallization and preliminary structure analysis of light harvesting II (B800-850) complex from the purple bacterium Rhodovulum sulfidophilum. J. Mol. Biol. 250: 1-10.

Savage, H., Cyrklaff, M., Montoya, G., Kuhlbrandt, W. & Sinning, I. (1995) Two-dimensional crystallisation and structure determination of LHII from Rhv. sulfidophilum at 7 Å resolution. in : Photosynthesis : from Light to Biosphere (Mathis, P., ed.) Kluwer Academic Publishers, Dordrecht, Netherlands, pp 239-242.

Leibl, W., Sinning, I., Ewald, G., Michel, H. & Breton, J. (1993) Biochem. 32: 1958-64.

Sinning, I., Kleywegt, G.J., Cowan, S.W., Reinemer, P., Dirr, H.W., Huber, R., Gilliland, G.L., Armstrong, R.N., Ji, X., Board, P., Olin, B., Mannervik, B. & Jones, T.A. (1993) J. Mol. Biol. 232: 192-212.

Sinning, I., Kleywegt, G.J., Mannervik, B., Board, P. & Jones, T.A. (1993) in (Tew, K.D. et al., eds) Structure and function of Glutathione transferases, CRC Press, pp. 75-86.

Mannervik, B., Berhane, K., Björnestedt, R., Board, P., Jones, T.A., Kolm, R.H., Olin, B., Sinning, I., Sroga, G.E., Stenberg, G., Tardioli, S. & Widersten, M. (1993) in (Tew, K.D. et al., eds) Structure and function of Glutathione transferases, CRC Press, pp. 29-38.

Divne C., Sinning, I., Stalberg, J., Pettersson, G., Bailey, M., Siika-aho, M., Margolles-Clark, E., Teeri, T. & Jones, T.A., Crystallization and preliminary X-ray studies on the core proteins of cellobiohydrolase I and endogluconase I from Trichoderma resei (1993) J. Mol. Biol. 234: 905-907.

Mathis, P., Sinning, I. & Michel, H. (1992) Biochem. Biophys. Acta 1098: 151-158.

Sinning, I. (1992) TIBS 17: 150-54.

Baciou, L., Sinning, I. & Sebban, P. (1991) Biochem. 30: 9110- 9116.

Sinning, I., Michel, H., Mathis, P. & Rutherford, A.W. (1990) FEBS Lett. 256: 192-194.

Sinning, I., Koepke, J., Schiller, B., Mathis, P., Rutherford, A.W. & Michel, H. (1990) in (M. Baltscheffsky, ed.) Current Research in Photosynthesis, Vol.I, Kluwer Acad. Publishers, Netherlands, pp. 173-176.

Sinning, I., Koepke, J., Schiller, B. & Michel , H. (1990) Z. Naturforsch. 45c: 455-458.

Michel, H., Sinning, I., Koepke, J., Ewald, G. & Fritzsch, G. (1990) Biochem. Biophys. Acta 1018: 115-118.

Sinning, I., Koepke, J. & Michel, H. (1990) in ( M. Michel-Beyerle, ed.) Springer Series in Biophysics: Reaction Center of Photosynthetic Bacteria Vol.6, Springer Verlag, pp. 199-208.

Sinning, I. (1989) Der Elektronenakzeptorkomplex im photosynthetischen Reaktionszentrum des Purpurbakteriums Rps. viridis , in Wissenschaftl. Forschungsbeiträge Biologie, Biochemie, Chemie, Bd. 32, Verlag Intemann, Prien a. Chiemsee, ISBN 3-926323-36-1.

Sinning, I., Michel, H., Mathis, P. & Rutherford, A.W. (1989) Biochem. 28: 5544-5553.

Parak, F., Birk, A., Frolov, E., Goldanskii, V., Sinning, I. & Michel, H. (1989) in (J. Jortner & B. Pullman, eds.) Perspectives in Photosynthesis, Kluwer Academic Publ., Netherlands, pp. 413- 421.

Sinning, I. (1989) Der Elektronenakzeptorkomplex im photosynthetischen Reaktionszentrum des Purpurbakteriums Rps. viridis , in Wissenschaftl. Forschungsbeiträge Biologie, Biochemie, Chemie, Bd. 32, Verlag Intemann, Prien a. Chiemsee, ISBN 3-926323-36-1.

Sinning, I., Michel, H., Mathis, P. & Rutherford, A.W. (1989) Biochem. 28: 5544-5553.

Parak, F., Birk, A., Frolov, E., Goldanskii, V., Sinning, I. & Michel, H. (1989) in (J. Jortner & B. Pullman, eds.) Perspectives in Photosynthesis, Kluwer Academic Publ., Netherlands, pp. 413- 421.

Sinning, I. & Michel, H. (1987) J. Biggins (ed.) Progress in Photosynthesis Res. Vol.III, Martinus Nijhoff Publ., Dordrecht,

Sinning, I. & Michel, H. (1987) Z. Naturforsch. 42c: 751-754.
  
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