Lipidomics
INSTRUMENTATION
QTRAP 5500
QSTAR Elite
Quattro II
OVERVIEW
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Lipidomics aims at investigating biological functions of lipids in health and disease. Although this research field has only recently emerged, it is rapidly expanding as lipids are increasingly recognized as important modulators of many intracellular processes, from regulation of protein function to modulation of cellular pathways. Cellular lipidomes contain thousands to ten of thousands individual molecular lipid species. Only nano-mass spectrometry provides a tool to deliver both qualitative and quantitative data on individual lipid species present in biological samples.
The Lipidomics facility builds on unique expertise in qualitative and quantitative lipid analysis by nano-mass spectrometry. Shotgun lipidomics allows to assess the lipid composition of either total membranes or protein-lipid-assemblies directly from extracts of biological samples. Applying automated sample infusion by mircofluidic-based nano-electrospray ionization high throughput measurements of up to 400 samples per series can be performed. Software specifically developed for automated spectra processing allows to rapidly identify and quantify individual lipid species. Including automated lipid extractions we aim at establishing a fully automated lipidomics approach, with the advantage of standardized work flows for the processing of high sample numbers. High throughput approaches can then be employed for comparative analyses of many samples, such as screenings for lipophilic markers indicative of pathological alterations of human serum or tissue samples.
PLATFORMS
Depending on the scientifc question, three complementary nano-platforms are available: 1) a hybrid quadrupol time-of-flight mass spectrometer, 2) a hybrid triple quadrupole linear ion trap mass spectrometer, and 3) a triple quadrupole mass spectrometer. The triple qudrupole system operates in single injection mode, whereas the other two instruments are coupled to a Nanomate devise. In addition, together with the ZMBH, an Orbitrap mass spectrometer is available.
Kontakt:
2969.jpg) | Britta Brügger Office: +49 6221 54-5426 Lab: +49 6221 54-4233  Fax: +49 6221 54-4366  |
