During their biosynthesis, RNA molecules undergo a vast number of co- and posttranscriptional processing and modification steps, which require dedicated enzyme machinery. We are particularly interested in protein-catalysed splicing of RNAs. This non-conventional splicing mechanism is an essential step during eukaryotic transfer RNA (tRNA) maturation and a mode of cellular stress signalling. We use an interdisciplinary approach from biochemistry and structural biology to cell biology to analyse the machinery and mechanisms underlying these processes.
The candidate will reconstitute the tRNA splicing machinery from yeast in vitro. She/he will use a combination of biochemical and structural methods to delineate the mechanisms of substrate recognition. In addition, the project aims at identifying interconnected RNA processing events using yeast cell biology and genetics.
Peschek J & Walter P (2019) tRNA ligase structure reveals kinetic competition between non-conventional mRNA splicing and mRNA decay. eLife 8:e44199. doi: 10.7554/eLife.44199.
Application Deadline: 31. Dez 2020
Start of PhD project: 01. Jan 2021 (earlier start date is possible)
Source of Funding:
Emmy Noether Programme (DFG), fully funded position until completion of thesis
We seek enthusiastic candidates with a special interest in RNA biology and structural biology. We look for applicants with a master or equivalent degree in biochemistry or any related field. Experience in protein biochemistry and molecular biology is required. Experience in structural biology and/or yeast genetics is a benefit.
Applications including a CV, a brief letter of motivation (1 page max), relevant certificates, and contact details of at least one academic referee should be submitted via email (single PDF file) to Jirka Peschek (firstname.lastname@example.org).