QTRAP 6500 (SelexIon/NanoMate)
QExactive (UPLC/NanoMate)
QTRAP 5500 (nUPLC/NanoMate)
QSTAR Elite (NanoMate)
Quattro II
OVERVIEW
Lipidomics aims at investigating biological functions of lipids in health and disease. Although this research field has only recently emerged, it is rapidly expanding as lipids are increasingly recognized as important modulators of many intracellular processes, ranging from regulation of protein function to modulation of cellular pathways. Cellular lipidomes contain thousands to ten of thousands individual molecular lipid species. Mass spectrometric lipid analysis provides a tool to obtain both qualitative and quantitative data on individual lipid species present in biological samples.
The Heidelberg Lipidomics is supported by CellNetworks (www.cellnetworks.uni-heidelberg.de) and builds on unique expertise in qualitative and quantitative lipid analysis by nano-mass spectrometry.
Shotgun lipidomics allows to assess the lipid composition of either total membranes or protein-lipid-assemblies directly from extracts of biological samples. Applying automated sample infusion by mircofluidic-based nano-electrospray ionization (ESI) high throughput measurements of up to 400 samples per series can be performed. In house developed/adapted software manage spectra processing for identification and quantification of individual lipid species.
Including automated lipid extractions we aim at establishing a fully automated lipidomics approach, with the advantage of standardized work flows for the processing of high sample numbers, such as in screenings for lipophilic marker to elucidate pathological alterations of biological samples.
PLATFORMS
Depending on the scientific question complementary nano-platforms are available based on e.g. hybrid quadrupole time-of-flight mass spectrometers, hybrid triple quadrupole linear ion trap mass spectrometers, triple quadrupole mass spectrometers and hybrid quadrupole-orbitrap mass spectrometers. Liquid and ion mobility chromatography systems are operated in online MS mode. Sample injection is performed by direct (manuel) injection or CHIP-based via a NanoMate system.
SELECTED REFERENCES
Faergeman SL, Evans H, Attfield KE, Desel C, Kuttikkatte SB, Sommerlund M, Jensen LT, Frokiaer J, Friese MA, Matthews PM, Lüchtenborg C, Brügger B, Oturai AB, Dendrou CA, Fugger L. A novel neurodegenerative spectrum disorder in patients with MLKL deficiency. Cell Death Dis. 2020 May 1;11(5):303.
Lüchtenborg C, Niederhaus B, Brügger B, Popovic B, Fricker G. Lipid Profiles of Five Essential Phospholipid Preparations for the Treatment of Nonalcoholic Fatty Liver Disease: A Comparative Study. Lipids. 2020 Apr 7. doi: 10.1002/lipd.12236.
Matthaeus C, Lahmann I, Kunz S, Jonas W, Melo AA, Lehmann M, Larsson E, Lundmark R, Kern M, Blüher M, Olschowski H, Kompa J, Brügger B, Müller DN, Haucke V, Schürmann A, Birchmeier C, Daumke O. EHD2-mediated restriction of caveolar dynamics regulates cellular fatty acid uptake. Proc Natl Acad Sci U S A. 2020 Mar 31;117(13):7471-7481.
Mücksch F, Citir M, Lüchtenborg C, Glass B, Traynor-Kaplan A, Schultz C, Brügger B, Kräusslich HG. Quantification of phosphoinositides reveals strong enrichment of PIP2 in HIV-1 compared to producer cell membranes. Sci Rep. 2019 Nov 27;9(1):17661.
Weingart OG, Eyer K, Lüchtenborg C, Sachsenheimer T, Brügger B, van Oostrum M, Wollscheid B, Dittrich PS, Loessner MJ. In vitro quantification of botulinum neurotoxin type A1 using immobilized nerve cell-mimicking nanoreactors in a microfluidic platform.
Analyst. 2019 Sep 23;144(19):5755-5765.
Hammerschmidt P, Ostkotte D, Nolte H, Gerl, MJ, Jais A, Brunner HL, Sprenger HG, Awazawa M, Nicholls HT, Turpin-Nolan SM, Langer T, Krüger M, Brügger B, Brüning JC. CerS6-Derived Sphingolipids Interact with Mff and Promote Mitochondrial Fragmentation in Obesity. Cell 2019 May 30; 177 (6): 1536-1552.
Wozny K, Lehmann WD, Wozny M, Akbulut BS, Brügger B. A method for the quantitative determination of glycerophospholipid regioisomers by UPLC-ESI-MS/MS. Anal Bioanal Chem. 2019 Feb;411(4):915-924. doi: 10.1007/s00216-018-1517-5.
Marx B, Hufbauer M, Zigrino P, Majewski S, Markiefka B, Sachsenheimer T, Brügger B, Akgül B. Phospholipidation of nuclear proteins by the human papillomavirus E6 oncoprotein: implication in carcinogenesis. Oncotarget. 2018 Sep 25;9(75):34142-34158.
Chowdhury A, Aich A, Jain G, Wozny K, Lüchtenborg C, Hartmann M, Bernhard O, Balleiniger M, Alfar EA, Zieseniss A, Toischer K, Guan K, Rizzoli SO, Brügger B, Fischer A, Katschinski DM, Rehling P, Dudek J. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia. Cell Rep. 2018 Oct 16;25(3):561-570.e6.
van Hensbergen VP, Movert E, de Maat V, Lüchtenborg C, Le Breton Y, Lambeau G, Payré C, Henningham A, Nizet V, van Strijp JAG, Brügger B, Carlsson F, McIver KS, van Sorge NM. Streptococcal Lancefield polysaccharides are critical cell wall determinants for human Group IIA secreted phospholipase A2 to exert its bactericidal effects. PLoS Pathog. 2018 Oct 15;14(10):e1007348.
Fledrich R, Abdelaal T, Rasch L, Bansal V, Schütza V, Brügger B, Lüchtenborg C, Prukop T, Stenzel J, Rahman RU, Hermes D, Ewers D, Möbius W, Ruhwedel T, Katona I, Weis J, Klein D, Martini R, Brück W, Müller WC, Bonn S, Bechmann I, Nave KA, Stassart RM, Sereda MW. Targeting myelin lipid metabolism as a potential therapeutic strategy in a model of CMT1A neuropathy. Nat Commun. 2018 Aug 2;9(1):3025.
Gruber A, Hornburg D, Antonin M, Krahmer N, Collado J, Schaffer M, Zubaite G, Lüchtenborg C, Sachsenheimer T, Brügger B, Mann M, Baumeister W, Hartl FU, Hipp MS, Fernández-Busnadiego R. Molecular and structural architecture of polyQ aggregates in yeast. Proc Natl Acad Sci U S A. 2018 Apr 10;115(15):E3446-E3453.
Zhang W, Neuner A, Rüthnick D, Sachsenheimer T, Lüchtenborg C, Brügger B, Schiebel E. Brr6 and Brl1 locate to nuclear pore complex assembly sites to promote their biogenesis. J Cell Biol. 2018 Mar 5; 217(3):877-894.
Dimitrov B, Himmelreich N, Hipgrave Ederveen AL, Lüchtenborg C, Okun JG, Breuer M, Hutter AM, Carl M, Guglielmi L, Hellwig A, Thiemann KC, Jost M, Peters V, Staufner C, Hoffmann GF, Hackenberg A, Paramasivam N, Wiemann S, Eils R, Schlesner M, Strahl S, Brügger B, Wuhrer M, Christoph Korenke G, Thiel C. Cutis laxa, exocrine pancreatic insufficiency and altered cellular metabolomics as additional symptoms in a new patient with ATP6AP1-CDG. Mol Genet Metab. 2018 Jan 27. pii: S1096-7192(17)30764-3.
Trautz B, Wiedemann H, Lüchtenborg C, Pierini V, Kranich J, Glass B, Kräusslich HG, Brocker T, Pizzato M, Ruggieri A, Brügger B*, Fackler OT. The host-cell restriction factor SERINC5 restricts HIV-1 infectivity without altering the lipid composition and organization of viral particles. J Biol Chem. 2017 Aug 18;292(33):13702-13713. doi: 10.107 4/jbc.M117.797332.
Kleinecke S, Richert S, de Hoz L, Brügger B, Kungl T, Asadollahi E, Quintes S, Blanz J, McGonigal R, Naseri K, Sereda MW, Sachsenheimer T, Lüchtenborg C, Möbius W, Willison H, Baes M, Nave KA, Kassmann CM. Peroxisomal dysfunctions cause lysosomal storage and axonal Kv1 channel redistribution in peripheral neuropathy. Elife. 2017 May 4;6. pii: e23332. doi: 10.7554/eLife.23332.
Ayyildiz D, Arga KY, Avci FG, Altinisik FE, Gurer C, Gulsoy Toplan G, Kazan D, Wozny K, Brügger B, Mertoglu B, Sariyar Akbulut B. Transcriptomic analysis displays the effect of (-)-roemerine on the motility and nutrient uptake in Escherichia coli. Curr Genet. 2016 Dec 24. doi: 10.1007/s00294-016-0673-4.
Luján P, Varsano G, Rubio T, Hennrich ML, Sachsenheimer T, Gálvez-Santisteban M, Martín-Belmonte F, Gavin AC, Brügger B, Köhn M. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J Cell Sci. 2016 Nov 1;129(21):4130-4142.
Sinzel M, Tan T, Wendling P, Kalbacher H, Özbalci C, Chelius X, Westermann B, Brügger B, Rapaport D, Dimmer KS. Mcp3 is a novel mitochondrial outer membrane protein that follows a unique IMP-dependent biogenesis pathway. EMBO Rep. 2016 Jul;17(7):965-81.
Gerl MJ, Bittl V, Kirchner K, Sachsenheimer T, Brunner HL, Lüchtenborg C, Özbalci C, Wiedemann H, Wegehingel S, Nickel W, Haberkant P, Schultz C, Krüger M and Brügger B. Sphingosine-1-phosphate lyase deficient cells as a tool to study protein lipid interactions. PlosOne, 2016;11(4):e0153009.
Callens N, Brügger B, Bonnafous P, Drobecq H, Gerl MJ, Krey T, Roman-Sosa G, Rümenapf T, Lambert O, Dubuisson J, Rouillé Y. Morphology and Molecular Composition of Purified Bovine Viral Diarrhea Virus Envelope. PLoS Pathog. 2016 Mar 3;12(3):e1005476.
Haberkant P, Stein F, Höglinger D, Gerl MJ, Brügger B, Van Veldhoven PP, Krijgsveld J, Gavin AC, Schultz C. Bifunctional Sphingosine for Cell-Based Analysis of Protein-Sphingolipid Interactions. ACS Chem Biol, 2016;11(1):222-30.
Dudek J, Cheng IF, Chowdhury A, Wozny K, Balleininger M, Reinhold R, Grunau S, Callegari S, Toischer K, Wanders RJ, Hasenfuß G, Brügger B, Guan K, Rehling P. Cardiac-specific succinate dehydrogenase deficiency in Barth syndrome. EMBO Mol Med. 2015 Dec 23;8(2):139-54.
Frenzel E, Wrenger S, Brügger B, Salipalli S, Immenschuh S, Aggarwal N, Lichtinghagen R, Mahadeva R, Marcondes AM, Dinarello CA, Welte T, Janciauskiene S. α1-Antitrypsin Combines with Plasma Fatty Acids and Induces Angiopoietin-like Protein 4 Expression. J Immunol. 2015;195(8):3605-16.
Hacke M, Björkholm P, Hellwig A, Himmels P, Ruiz de Almodovar C, Brügger B, Wieland F, Ernst AM Inhibition of Ebola virus glycoprotein-mediated cytotoxicity by targeting its transmembrane domain and cholesterol. Nature Comm 2015;6:7688.
Dorninger F, Brodde A, Braverman NE, Moser AB, Just WW, Forss-Petter S, Brügger B, Berger J. Homeostasis of phospholipids - The level of phosphatidylethanolamine tightly adapts to changes in ethanolamine plasmalogens. Biochim Biophys Acta. 2015 Feb;1851(2):117-28.
Harner ME, Unger AK, Izawa T, Walther DM, Ozbalci C, Geimer S, Reggiori F, Brügger B, Mann M, Westermann B, Neupert W. Aim24 and MICOS modulate respiratory function, tafazzin-related cardiolipin modification and mitochondrial architecture. Elife. 2014 Jan 1;3:e01684.
Gerl MJ, Sachsenheimer T, Grzybek M, Coskun U, Wieland FT, Brügger B. Analysis of transmembrane domains and lipid modified peptides with matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. Anal Chem. 2014 Apr 15;86(8):3722-6.
Brügger B. Lipidomics: analysis of the lipid composition of cells and subcellular organelles by electrospray ionization mass spectrometry. Annu Rev Biochem. 2014 Jun 2;83:79-98.
Küch EM, Vellaramkalayil R, Zhang I, Lehnen D, Brügger B, Sreemmel W, Ehehalt R, Poppelreuther M, Füllekrug J. Differentially localized acyl-CoA synthetase 4 isoenzymes mediate the metabolic channeling of fatty acids towards phosphatidylinositol. Biochim Biophys Acta. 2014 Feb;1841(2):227-39.
Özbalci C, Sachsenheimer T, Brügger B. Quantitative analysis of cellular lipids by nano-electrospray ionisation mass spectrometry. Methods Mol Biol. 2013;1033:3-20.
Haberkant P, Raijmakers R, Wildwater M, Sachsenheimer T, Brügger B, Maeda K, Houweling M, Gavin AC, Schultz C, van Meer G, Heck AJ, Holthuis JC. In vivo profiling and visualization of cellular protein-lipid interactions using bifunctional fatty acids. Angew Chem Int Ed Engl. 2013;52(14):4033-8.
Tan T, Ozbalci C, Brügger B, Rapaport D, Dimmer KS. Mcp1 and Mcp2, two novel proteins involved in mitochondrial lipid homeostasis. J Cell Sci. 2013;126(Pt 16):3563-74.
Lorizate M, Sachsenheimer T, Glass B, Habermann A, Gerl MJ, Kräusslich HG, Brügger B. Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines. Cell Microbiol. 2013;15(2):292-304.
Krijnse Locker J, Chlanda P, Sachsenheimer T, Brügger B. Poxvirus membrane biogenesis: rupture not disruption. Cell Microbiol. 2013;15(2):190-9.
Duran JM, Campelo F, van Galen J, Sachsenheimer T, Sot J, Egorov MV, Rentero C, Enrich C, Polishchuk RS, Goñi FM, Brügger B, Wieland F, Malhotra V. Sphingomyelin organization is required for vesicle biogenesis at the Golgi complex. EMBO J. 2012;31(24):4535-46.
Krumpe K, Frumkin I, Herzig Y, Rimon N, Özbalci C, Brügger B, Rapaport D, Schuldiner M. Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Mol Biol Cell. 2012;23(20):3927-35.
Zhao H, Williams DE, Shin JB, Brügger B, Gillespie PG. Large membrane domains in hair bundles specify spatially constricted radixin activation. J Neurosci. 2012;32(13):4600-9.
Brodde A, Teigler A, Brügger B, Lehmann WD, Wieland F, Berger J, Just WW. Impaired neurotransmission in ether lipid-deficient nerve terminals. Hum Mol Genet. 2012;21(12):2713-24.
Contreras FX, Ernst AM, Haberkant P, Björkholm P, Lindahl E, Gönen B, Tischer C, Elofsson A, von Heijne G, Thiele C, Pepperkok R, Wieland F, Brügger B. Molecular recognition of a single sphingolipid species by a protein's transmembrane domain. Nature 2012;481(7382):525-9.
Haag M, Schmidt, A, Sachsenheimer, T, Brügger B. Quantification of Signaling Lipids by Nano-Electrospray Ionization Tandem Mass Spectrometry (Nano-ESI MS/MS). Metabolites 2012, 2(1), 57-76.
Schmidt A, Oberle N, Weiß EM, Vobis D, Frischbutter S, Baumgrass R, Falk CS, Haag M, Brügger B, Lin H, Mayr GW, Reichardt P, Gunzer M, Suri-Payer E, Krammer PH. Human Regulatory T Cells Rapidly Suppress T Cell Receptor-Induced Ca2+, NF-κB, and NFAT Signaling in Conventional T Cells.
Sci Signal. 2011;4(204):ra90.
Sancar G, Sancar C, Brügger B, Ha N, Sachsenheimer T, Gin E, Wdowik S, Lohmann I, Wieland F, Höfer T, Diernfellner A, Brunner M. A global circadian repressor controls antiphasic expression of metabolic genes in Neurospora. Mol Cell. 2011;44(5):687-97.
Yurlova L, Kahya N, Aggarwal S, Kaiser HJ, Chiantia S, Bakhti M, Pewzner-Jung Y, Ben-David O, Futerman AH, Brügger B, Simons M. Self-segregation of myelin membrane lipids in model membranes. Biophys J. 2011;101(11):2713-20.
Ritzerfeld J, Remmele S, Wang T, Temmerman K, Brügger B, Wegehingel S, Tournaviti S, Strating JR, Wieland FT, Neumann B, Ellenberg J, Lawerenz C, Hesser J, Erfle H, Pepperkok R, Nickel W. Phenotypic profiling of the human genome reveals gene products involved in plasma membrane targeting of SRC kinases. Genome Res. 2011;21(11):1955-68.
Ben-David O, Pewzner-Jung Y, Brenner O, Laviad EL, Kogot-Levin A, Weissberg I, Biton IE, Pienik R, Wang E, Kelly S, Alroy J, Raas-Rothschild A, Friedman A, Brügger B, Merrill AH Jr, Futerman AH. Encephalopathy caused by ablation of very long acyl chain ceramide synthesis may be largely due to reduced galactosylceramide levels. J Biol Chem. 2011;286 (34):30022-33.
Habermehl D, Parkitna JR, Kaden S, Brügger B, Wieland F, Gröne HJ, Schütz G. Glucocorticoid Activity during Lung Maturation Is Essential in Mesenchymal and Less in Alveolar Epithelial Cells. Mol Endocrinol. 2011;25(8):1280-8.
Kou J, Kovacs GG, Höftberger R, Kulik W, Brodde A, Forss-Petter S, Hönigschnabl S, Gleiss A, Brügger B, Wanders R, Just W, Budka H, Jungwirth S, Fischer P, Berger J. Peroxisomal alterations in Alzheimer's disease. Acta Neuropathol. 2011;122(3):271-83.
Welti S, Kühn S, D'Angelo I, Brügger B, Kaufmann D, Scheffzek K. Structural and biochemical consequences of NF1 associated nontruncating mutations in the Sec14-PH module of neurofibromin. Hum Mutat. 2011;32(2):191-7.
Merz A, Long G, Hiet MS, Brügger B, Chlanda P, Andre P, Wieland F, Krijnse-Locker J, Bartenschlager R Biochemical and morphological properties of hepatitis C virus particles and determination of their lipidome. J Biol Chem. 2011;286(4):3018-32.
Förster S, Persch E, Tverskoy O, Rominger F, Helmchen G, Klein C, Gönen B, Brügger B. Syntheses and Biological Properties of Brefeldin Analogues. Eur. J. Org. Chem. 2011, 5, 878–891
Osman C, Haag M, Wieland FT, Brügger B, Langer T. A mitochondrial phosphatase required for cardiolipin biosynthesis: the PGP phosphatase Gep4. EMBO J. 2010;29(12):1976-87.
Erez E, Stjepanovic G, Zelazny AM, Brügger B, Sinning I, Bibi E. Genetic evidence for functional interaction of the Escherichia coli signal recognition particle receptor with acidic lipids in vivo. J Biol Chem. 2010;285(52):40508-14.
Mora R, Dokic I, Kees T, Hüber CM, Keitel D, Geibig R, Brügger B, Zentgraf H, Brady NR, Régnier-Vigouroux A. Sphingolipid rheostat alterations related to transformation can be exploited for specific induction of lysosomal cell death in murine and human glioma. Glia 2010;58(11):1364-83.
Pewzner-Jung Y, Park H, Laviad EL, Silva LC, Lahiri S, Stiban J, Erez-Roman R, Brügger B, Sachsenheimer T, Wieland F, Prieto M, Merrill AH Jr, Futerman AH. A critical role for ceramide synthase 2 in liver homeostasis: I. alterations in lipid metabolic pathways. J Biol Chem. 2010;285(14):10902-10.
Osman C, Haag M, Potting C, Rodenfels J, Dip PV, Wieland FT, Brügger B, Westermann B, Langer T. The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol. 2009;184(4):583-96.
Lorizate M, Brügger B, Akiyama H, Glass B, Müller B, Anderluh G, Wieland FT, Kräusslich HG. Probing HIV-1 membrane liquid order by Laurdan staining reveals producer cell-dependent differences. J Biol Chem. 2009;284(33):22238-47.
Saher G, Quintes S, Möbius W, Wehr MC, Krämer-Albers EM, Brügger B, Nave KA. Cholesterol regulates the endoplasmic reticulum exit of the major membrane protein P0 required for peripheral myelin compaction. J Neurosci. 2009;29(19):6094-104.
Trajkovic K, Hsu C, Chiantia S, Rajendran L, Wenzel D, Wieland F, Schwille P, Brügger B, Simons M. Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science 2008;319(5867):1244-7.
Haberkant P, Schmitt O, Contreras FX, Thiele C, Hanada K, Sprong H, Reinhard C, Wieland FT, Brügger B. Protein-sphingolipid interactions within cellular membranes. J Lipid Res. 2008;49(1):251-62.
Kwa LG, Wegmann D, Brügger B, Wieland FT, Wanner G, Braun P. Mutation of a single residue, beta-glutamate-20, alters protein-lipid interactions of light harvesting complex II. Mol Microbiol. 2008;67(1):63-77.
Brügger B, Krautkrämer E, Tibroni N, Munte CE, Rauch S, Leibrecht I, Glass B, Breuer S, Geyer M, Kräusslich HG, Kalbitzer HR, Wieland FT, Fackler OT. Human immunodeficiency virus type 1 Nef protein modulates the lipid composition of virions and host cell membrane microdomains. Retrovirology 2007;4:70.
Kassmann CM, Lappe-Siefke C, Baes M, Brügger B, Mildner A, Werner HB, Natt O, Michaelis T, Prinz M, Frahm J, Nave KA. Axonal loss and neuroinflammation caused by peroxisome-deficient oligodendrocytes. Nat Genet. 2007;39(8):969-76.
Findeisen F, Linder JU, Schultz A, Schultz JE, Brügger B, Wieland F, Sinning I, Tews I. The structure of the regulatory domain of the adenylyl cyclase Rv1264 from Mycobacterium tuberculosis with bound oleic acid. J Mol Biol. 2007;369(5):1282-95.
Takamori S, Holt M, Stenius K, Lemke EA, Grønborg M, Riedel D, Urlaub H, Schenck S, Brügger B, Ringler P, Müller SA, Rammner B, Gräter F, Hub JS, De Groot BL, Mieskes G, Moriyama Y, Klingauf J, Grubmüller H, Heuser J, Wieland F, Jahn R. Molecular anatomy of a trafficking organelle. Cell 2006;127(4):831-46.
Brügger B, Glass B, Haberkant P, Leibrecht I, Wieland FT, Kräusslich HG. The HIV lipidome: a raft with an unusual composition. Proc Natl Acad Sci USA 2006;103(8):2641-6.
Saher G, Brügger B, Lappe-Siefke C, Möbius W, Tozawa R, Wehr MC, Wieland F, Ishibashi S, Nave KA. High cholesterol level is essential for myelin membrane growth. Nat Neurosci. 2005;8(4):468-75.
Schneiter R, Brügger B, Amann CM, Prestwich GD, Epand RF, Zellnig G, Wieland FT, Epand RM. Identification and biophysical characterization of a very-long-chain-fatty-acid-substituted phosphatidylinositol in yeast subcellular membranes. Biochem J. 2004;381(Pt 3):941-9.
Brügger B, Graham C, Leibrecht I, Mombelli E, Jen A, Wieland F, Morris R. The membrane domains occupied by glycosylphosphatidylinositol-anchored prion protein and Thy-1 differ in lipid composition. J Biol Chem. 2004;279(9):7530-6.
Neumann S, Petfalski E, Brügger B, Grosshans H, Wieland F, Tollervey D, Hurt E. Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 2003;4(12):1156-62.
Rodemer C, Thai TP, Brügger B, Kaercher T, Werner H, Nave KA, Wieland F, Gorgas K, Just WW.Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice. Hum Mol Genet. 2003;12(15):1881-95.
Young ME, Karpova TS, Brügger B, Moschenross DM, Wang GK, Schneiter R, Wieland FT, Cooper JA. The Sur7p family defines novel cortical domains in Saccharomyces cerevisiae, affects sphingolipid metabolism, and is involved in sporulation. Mol Cell Biol. 2002;22(3):927-34.
Gkantiragas I, Brügger B, Stüven E, Kaloyanova D, Li XY, Löhr K, Lottspeich F, Wieland FT, Helms JB. Sphingomyelin-enriched microdomains at the Golgi complex. Mol Biol Cell 2001;12(6):1819-33.
Brügger B, Sandhoff R, Wegehingel S, Gorgas K, Malsam J, Helms JB, Lehmann WD, Nickel W, Wieland FT. Evidence for segregation of sphingomyelin and cholesterol during formation of COPI-coated vesicles. J Cell Biol. 2000;151(3):507-18.
Schneiter R, Brügger B, Sandhoff R, Zellnig G, Leber A, Lampl M, Athenstaedt K, Hrastnik C, Eder S, Daum G, Paltauf F, Wieland FT, Kohlwein SD. Electrospray ionization tandem mass spectrometry (ESI-MS/MS) analysis of the lipid molecular species composition of yeast subcellular membranes reveals acyl chain-based sorting/remodeling of distinct molecular species en route to the plasma membrane. J Cell Biol. 1999;146(4):741-54.
Ledesma MD, Brügger B, Bünning C, Wieland FT, Dotti CG. Maturation of the axonal plasma membrane requires upregulation of sphingomyelin synthesis and formation of protein-lipid complexes. EMBO J. 1999;18(7):1761-71.
Sandhoff R, Brügger B, Jeckel D, Lehmann WD, Wieland FT. Determination of cholesterol at the low picomole level by nano-electrospray ionization tandem mass spectrometry. J Lipid Res. 1999;40(1):126-32.
Brügger B, Erben G, Sandhoff R, Wieland FT, Lehmann WD. Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry. Proc Natl Acad Sci USA 1997;94(6):2339-44.
contact:
Britta Brügger
britta.bruegger@bzh.uni-heidelberg.de