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Ed Hurt 
Nuclear pore complex and ribosome assembly

Groupleader: Ed Hurt

List of Publications


J. Baßler, E. Hurt:
Eukaryotic Ribosome Assembly.
Annu Rev Biochem. 2018 Dec 19. doi: 10.1146/annurev-biochem-013118-110817. (Epub ahead of print)

Matthias Thoms, Valentin Mitterer, Lukas Kater, L. Falquet, Roland Beckmann, Dieter Kressler
and Ed Hurt:
Suppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal
5S RNP-rotation.
Nat Commun. 2018 Oct 5;9(1):4094. doi: 10.1038/s41467-018-06660-w.

N. Mücke, L. Kämmerer, S. Winheim, R. Kirmse, J. Krieger, M. Mildenberger, J. Baßler, E. Hurt, WH Goldmann, U. Aebi, K. Toth, J. Langowski, H. Hermann:
Assembly Kinetics of Vimentin Tetramers to Unit-Length Filaments: A Stopped-Flow Study.
Biophys J. 2018 May 22;114(10):2408-2418. doi: 10.1016/j.bpj.2018.04.032. Epub 2018 May 10

Jan Schuller, Sebastian Falk, Lisa Fromm, Ed Hurt and Elena Conti:
Structure of the nuclear exosome captured on a maturing preribosome.
Science. 2018 Apr 13;360(6385):219-222. doi: 10.1126/science.aar5428. Epub 2018 Mar 8.


Lukas Kater, Matthias Thoms, Clara Barrio Garcia, Jingdong Cheng, Sherif Ismail, Yasar Luqman Ahmed, Gert Bange, Dieter Kressler, Otto Berninghausen, Irmi Sinning, Ed Hurt and Roland Beckmann: Visualizing the earliest steps during nucleolar pre-60S ribosome assembly
Cell 171, 1599–1610 (2017).

André Heuer, Emma Thomson, Christian Schmidt, Otto Berninghausen, Thomas Becker,
Ed Hurt and Roland Beckmann:
Eukaryotic ribosome assembly, transport and quality control
Elife 6, doi: 10.7554/eLife.30189 (2017).

Sebastian Falk, Jan-Niklas Tants, Ed Hurt, Michael Sattler and Elena Conti:
Structural insights into the interaction of the nuclear exosome helicase Mtr4
with the pre-ribosomal protein Nop53
RNA 23, 1780-1787. doi: 10.1261/rna.062901.117 (2017).

Bebiana Sá-Moura, Markus Kornprobst, Satyavati Kharde, Yasar Luqman Ahmed,
Gunter Stier, Ruth Kunze, Irmgard Sinning and Ed Hurt:
Mpp10 represents a platform for the interaction of multiple factors from the 90S pre-ribosome
PLOS One 12, doi: 10.1371/journal.pone.0183272 (2017).

Jingdong Cheng, Nikola Kellner, Otto Berninghausen, Ed Hurt and Roland Beckmann:
3.2 Angstrœm resolution structure of the 90S pre-ribosome before A1 pre-rRNA cleavage
Nat. Struct. Mol. Biol. doi:10.1038/nsmb.3476 (2017).

Dieter Kressler, Ed Hurt and Jochen Bassler:
A puzzle of life: crafting ribosomal subunits
Trends Biochem. Sci. , in press (2017).

Cohue Peña, Ed Hurt and Vikram Govind Panse:
Eukaryotic ribosome assembly, transport and quality control
Nat. Struct. Mol. Biol. 24, 689-699. doi: 10.1038/nsmb.3454 (2017).

Roman Teimer, Jan Kosinski, Alexander von Appen, Martin Beck and Ed Hurt:
Short linear motifs in scaffold Nups act as sensors to cooperatively
connect pre-assembled modules during NPC biogenesis
Nature Commun. 8, 1107. doi: 10.1038 (2017).

Lisa Fromm, Sebastian Falk, Dirk Flemming, Jan Schuller, Matthias Thoms,
Elena Conti and Ed Hurt:
Reconstitution of the complete pathway of of ITS2 processing at the pre-ribosome
Nature Commun., in press (2017).

Miriam Sturm, Jingdong Cheng, Jochen Bassler, Roland Beckmann and Ed Hurt:
Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly
Nature Commun., in press (2017).

Fabiola R. Calviño, Markus Kornprobst, Géza Schermann, Fabienne Birkle, Gunter Stier,
Klemens Wild, Ed Hurt, Yasar L. Ahmed and Irmgard Sinning:
Structural basis for 5‘-ETS recognition by Utp4 at the early stages of ribosome biogenesis
PLOS One, doi: 10.1371/journal.pone.0178752 (2017).

Anshuk Sarkar, Matthias Thoms, Clara Barrio-Garcia, Emma Thomson, Dirk Flemming,
Roland Beckmann and Ed Hurt:
Pre-ribosomes escaping the nucleus are caught during translation by
cytoplasmic quality control quality control mechanisms
Nat. Struct. Mol. Biol., doi: 10.1038/nsmb.349 (2017).

Martin Beck and Ed Hurt:
The nuclear pore complex: understanding its function through structural insight
Nature Rev. Mol. Cell Biol. 18, 73-89 (2017).

Jochen Baßler, Yasar Luqman Ahmed, Martina Kallas, Markus Kornprobst, Fabiola Rodriguez-Calvino, Marén Gnädig, Matthias Thoms, Gunter Stier,Sherif Ismail, Satyavati Kharde, Nestor Castillo, Sabine Griesel, Sonja Bastuck, Bettina Bradatsch, Emma Thomson, Dirk Flemming, Irmi Sinning, Ed Hurt:
Interaction network of the ribosome assembly machinery from a  eukaryotic thermophile
Protein Sci. 26, 327-342. doi: 10.1002/pro.3085 (2017).


Anshuk Sarkar, Markus Pech, Matthias Thoms, Dirk Flemming, Roland Beckmann and Ed Hurt: Coupling ribosome stalk biogenesis with nuclear export factor recruitment to the nascent 60S subunit
Nat. Struct. Mol. Biol. 23, 1074-1082 (2016).

Jan Kosinski, Shyamal Mosalaganti, Alexander von Appen, Roman Teimer, Amanda DiGuilio, William Wan, Huy Khahn Bui, Wim J.H. Hagen, John A.G. Briggs, Joseph S. Glavy, Ed Hurt and Martin Beck:
Molecular architecture of the inner ring scaffold of the human nuclear pore complex.
Science 352, 363-365. doi: 10.1126/science.aaf0643 (2016).

Markus Kornprobst, Martin Turk, Nikola Kellner, Jing-Dong Cheng, Dirk    Flemming, Martin Koš, Isabelle Koš, Matthias Thoms, Otto Berninghausen,  Roland Beckmann and Ed Hurt:
Architecture of the 90S pre-ribosome: a structural view on the birth of the eukaryotic ribosome
Cell 166, 380–393 (2016).

George Manikas, Emma Thomson and Ed Hurt:
Role of cation-dependent Nug1 GTPase in recruiting Dbp10 helicase to the undeveloped peptidyl transferase center of the pre-ribosome.
Nucl Acid Res. doi: 10.1093/nar/gkw045 (2016).

Nikola Kellner, Johannes Schwarz, Miriam Sturm, Javier Fernandez-Martinez,
Sabine Griesel, Wenzhu Zhang, Brian Chait, Michael P. Rout, Ulrich Kück and Ed Hurt:
Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies.
Sci Rep. 5:15401. doi: 10.1038/srep15401 (2016).

Matthias Thoms, Yasar Luqman Ahmed, Karthik Maddi, Ed Hurt and Irmgard Sinning:
Concerted removal of the Erb1-Ytm1 complex in ribosome biogenesis relies on an elaborate interface.
Nucl Acid Res. 44, 926-39. doi: 10.1093/nar/gkv1365 (2016).

Clara Barrio-Garcia, Matthias Thoms, Dirk Flemming, Lukas Kater, Jochen Bassler, Roland Beckmann and Ed Hurt:
Architecture of a ribosome assembly bound to a combined remodeling-checkpoint machinery.
Nature Struct. Mol. Biol. 23, 37-44 doi:10.1038/nsmb.3132 (2016).


Lisa Gasse, Dirk Flemming and Ed Hurt: Coordinated ITS2 rRNA processing  by the Las1 complex integrating endonuclease, polynucleotide kinase and  exonuclease activities
Mol. Cell, 60(5):808-15. doi: 10.1016/j.molcel.2015.10.021. (2015).

Elisa Pesce, Claudia Minici, JochenBassler, Ed Hurt, Massimo Degano, Piera  Calamita and Stefano Biffo: Direct and high throughput (HT) interactions on  the ribosomal surface by iRIA
Sci Rep. 5:15401. doi: 10.1038/srep15401 (2015).

Tobias Stuwe, Christopher J. Bley, Karsten Thierbach, Stefan Petrovic,  Sandra Schilbach, Daniel J. Mayo, Thibaud Perriches, Emily J. Rundlet,  Young Jeon, Leslie N. Collins, Daniel H. Lin, Marcin Paduch, Akiko Koide,  Vincent Lu, Jessica Fischer, Ed Hurt, Shohei Koide, Anthony A. Kossiakoff,  André Hoelz: Architecture of the nuclear pore inner ring complex
Science 350, 56-64 (2015).

Jessica Fischer, Roman Teimer, Stefan Amlacher, Ruth Kunze and Ed Hurt:  Linker Nups connect the nuclear pore complex inner ring with outer ring and  transport channel
Nature Struct. Mol. Biol. 22, 774-81 (2015).

Clara Barrio-Garcia, Matthias Thoms, Dirk Flemming, Lukas Kater, Jochen  Bassler, Roland Beckmann and Ed Hurt: Architecture of a ribosome assembly  bound to a combined remodeling-checkpoint machinery
Nature Struct. Mol. Biol, accepted (2015).

Patrick Pausch Ujjwala Singh, Yasar Luqman Ahmed, Florian Altegoer,  Gunter Stier, Matthias Thoms, Ed Hurt, Irmgard Sinning, Gert Bange and  Dieter Kressler: Co-translational capturing of nascent ribosomal proteins by  their dedicated chaperones
Nat. Comm. 6, 7494 (2015).

Shintaro Aibara, Eugene Valkov, Meindert Lamers, Lyudimila Dimitrova, Ed Hurt
and Murray Stewart:
Structural characterization of the principal mRNA export factor Mex67:Mtr2from
Chaetomium thermophilum.
Acta Cryst.F71, 876-888 (2015).

Jochen Baßler und Ed Hurt:
Einblicke in die 'Kinderstube' während der Ribosomen-Entwicklung.
Biospektrum (Springer Verlag) 02.15, 144-147 (2015).

Ed Hurt und Martin Beck:
Toward understanding nuclear pore complex architecture and dynamics in the age of
integrative structural analysis
Curr. Opin. Cell Biol. 34:31-38. doi: 10.1016/j.ceb.2015.04.009 (2015).

Jun Katahira, Lyudmila Dimitrova, Yumiko Imai, Ed Hurt:
NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export.
Nucl. Acid Res 3, 1894-904 (2015).

Matthias Thoms, Emma Thomson Jochen Baßler, Marén Gnädig, Sabine  Griesel and Ed Hurt: The exosome is recruited to RNA substrates through  specific adapter proteins.
Cell 162, 1029-1038 (2015).

Philipp Stelter, Ferdinand Huber, Ruth Kunze, Dirk Flemming, André Hoelz and Ed Hurt:
Coordinated ribosomal L4 protein assembly into the pre-ribosome is regulated by
its eukaryote-specific extension.
Mol. Cell 58, 854-862 (2015).

Fabiola R. Calviño, Satyavati Kharde, A. Ori, Astrid Hendricks, Klemens Wild,
Dieter Kressler, Gert Bange, Ed Hurt, Martin Beck and Irmgard Sinning:
Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an
essential rRNA-binding site.
Nature Com. 6:6510. doi: 10.1038/ncomms7510 (2015).

Lyudmila Dimitrova, Eugine Valkow, Shinataro Aibara, Dirk Flemming, Steven McLaughlin,
Ed Hurt and Murray Stewart:
Characterization of the Chaetomium thermophilum TREX-2 complex and its interaction
with the mRNA nuclear export factor MEX67:MTR2.
Structure 23, 1246-1257 (2015).

Monika Gaik, Dirk Flemming, Alexander von Appen, Panagiotis Kastritis, Philipp Stelter,
Khanh Huy Bui, Alessandro Ori, Jochen Bassler, Jessica Fischer, Elisar Barbar, Martin Beck and Ed Hurt:
Structural basis for assembly and function Nup82 complex in the nuclear pore scaffold.
J. Cell Biol. 208, 283-297 doi/10.1083/jcb.201411003 (2015).


Thomas Bock, Wei-Hua Chen, Alessandro Ori, Nayab Malik, Noella da Silva,  Jaime Huerta Cepas, Sean Powell, Panagiotis Kastritis, Georgy  Smyshlyae, Ivana Vonkova, Joanna Kirkpatrick, Tobias Doerks, Leo Nesme,  Jochen Bassler, Martin Kos, Ed Hurt, Teresa Carlomagno, Anne Claude  Gavin, Orsolya Barabas, Christoph Müller, Vera van Noort, Martin Beck and  Peer Bork: An integrated approach for genome annotation of the eukaryotic  thermophile Chaetomium thermophilum
Nucl. Acid Res., published online November 14, 2014     doi:10.1093/nar/gku1147 (2014).

Jochen Baßler, Helge Paternoga, Iris Holdermann, Matthias Thoms, Sander  Granneman, Clara Barrio Garcia, Afua Nyarko, Gunther Stier, Sarah A. Clark,  Daniel Schraivogel, Martina Kallas, Roland Beckmann, David Tollervey, Elisar  Barbar, Irmi Sinning and Ed Hurt: A network of assembly factors involved  is involved in remodeling rRNA elements during pre-ribosome maturation
J. Cell Biol., Nov 17. pii: jcb.201408111 (2014).

Philipp Stelter and Ed Hurt: A pulse-chase epitope labeling to study cellular  dynamics of newly synthesized proteins: a novel strategy to characterize NPC  biogenesis and ribosome maturation/export
Methods in Cell Biol. 122C,147-163. doi: 10.1016/B978-0-12-417160-2.00007-2 (2014).

Dirk Flemming, Philipp Stelter and Ed Hurt: Utilizing the Dyn2-dimerization- zipper as a tool to probe NPC structure and function
Methods in Cell Biol. 122C, 99-115. doi: 10.1016/B978-0-12-417160-2.00005-9 (2014).

Holger Webert, Sven Freibert, Angelo Gallo, Torsten Heidenreich, Uwe Linne,
Stefan Amlacher, Ed Hurt, Ulrich Mühlenhoff, Lucia Banci and Roland Lill: Functional reconstitution of de novo mitochondrial Fe/S cluster synthesis  reveals an essential role of ferredoxin
Nature Com. 5, doi:10.1038/ncomms6013 (2014).

Sébastien Ferreira-Cerca, Irene Kiburu, Emma Thomson, Nicole Laronde- Leblanc and Ed Hurt: Catalytic Rio1 kinase/ATPase mutant induces trapping  of late pre-40S biogenesis factors in 80S-like ribosomes
Nucl. Acid Res. 1, doi: 10.1093/nar/gku542 (2014).

Christoph Leidig, Matthias Thoms, Iris Holdermann, Bettina Bradatsch, Otto Berninghausen,
Gert Bange, Irmgard Sinning, Ed Hurt and Roland Beckmann:
Construction of the central protuberance requires rotation of 5S RNP during
60S ribosome biogenesis.
Nature Com. 5, 3491  DOI: 10.1038 (2014).

Tobias Stuwe, Daniel H. Lin, Leslie Collins, Ed Hurt and André Hoelz:
Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192
and karyopherins.
Proc. Natl. Acad. Sci. USA 18, 2530-2535 (2014).

Yoshitaka Matsuo, Sander Granneman, Matthias Thoms, Rizos-Georgios Manikas,
David Tollervey and Ed Hurt:
Coupled GTPase and remodeling ATPase activities form a checkpoint for ribosome export.
Nature 505, 112-116 (2014).


Christoph Leidig, Gert Bange, Jürgen Kopp, Stefan Amlacher, Ajay Aravind, Stephan Wickles,
Gregor Witte, Ed Hurt, Roland Beckmann and Irmgard Sinning:
Structural characterization of a eukaryotic co-translational chaperone, the ribosome-associated complex (RAC).
Nat. Struct. Mol. Biol 20, 23-28 (2013).

Vera van Noort, Bettina Bradatsch, Manimozhiyan Arumugam, Stefan Amlacher, Gerd Bange,
Chris Creevey, Sebastien Falk, Daniel R. Mende, Irmgard Sinning, Ed Hurt and Peer Bork:
Consistent mutational paths predict eukaryotic thermostability.
BMC Evolutionary Biology 13:7 doi:10.1186/1471-2148-13-7 (2013).

Thomas Monecke, David Haselbach, Piotr Neuman, Emma Thomson, Ed Hurt, Holger Stark,
Achim Dickmanns and Ralf Ficner:
Structures of the nuclear export receptor CRM1 in its free state reveal major structural
rearrangements upon cargo binding.
Proc. Natl. Acad. Sci. USA 110, 960-965 (2013).

Thomas Hierlmeier, Juliane Merl, Martina Sauert, Jorge Perez-Fernandez, Patrick Schultz,
Astrid Bruckmann, Stephan Hamperl, Uli Ohmayer, Joachim Griesenbeck, Reinhard Rachel,
Anja Jakob, Kristin Hergert, Rainer Deutzmann, Ed Hurt, Philipp Milkereit, Jochen Baßler
and Herbert Tschochner:
Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit
precursors in S. cerevisiae.
Nucl. Acid Res. 41, 1191-1200 (2013).

Karsten Thierbach, Alexander von Appen, Matthias Thoms, Martin Beck, Dirk Flemming
and Ed Hurt:
Protein interfaces of the Conserved Nup84 Complex from Chaetomium thermophilum
revealed by cross-linking mass spectrometry and electron microscopy.
Structure 21, 9, 1672-1682 (2013).

Maria Hondele, Tobias Stuwe, Markus Hassler, Felix Halbach, Bianca Nijmeijer, Vladimir Rybin,
Stefan Amlacher, Ed Hurt and Andreas Ladurner:
Structural basis of histone H2A-H2B recognition by the essential chaperone FACT.
Nature 499, 111-114 (2013).

Gert Bange, Guillaume Murat, Irmgard Sinning, Ed Hurt, and Dieter Kressler:
Streamlining ribosome assembly by synchronized nuclear import of ribosomal proteins.
Communicative & Integrative Biology 6, e24792. doi: 10.4161/cib24792 (2013).

Emma Thomson, Sébastien Ferreira-Cerca and Ed Hurt:
Eukaryotic Ribosome Biogenesis.
J. Cell Sci. in press (2013).


Bettina Bradatsch, Christoph Leidig, Sander Granneman, Marén Gnädig, Bettina Böttcher, David Tollervey, Roland Beckmann and Ed Hurt:
Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel.
Nat. Struct. Mol. Biol 19, 1234-1241 (2012).

Philipp Stelter, Ruth Kunze, Monika Radwan, Emma Thomson, Karsten Thierbach,
Matthias Thoms and Ed Hurt:
Monitoring Spatiotemporal Biogenesis of Macromolecular Assemblies by Pulse-chase Epitope Labeling.
Mol. Cell 47, 788-796 (2012).

Dieter Kressler, Gert Bange, Yutaka Ogawa, Dagmar Pratte, Bettina Bradatsch, Daniela Roser,
Jun Katahira, Irmgard Sinning, and Ed Hurt:
Synchronizing Nuclear Import of Ribosomal Proteins with Ribosome Assembly.
Science Vol. 338 no. 6107, 666-671 (2012).

Sébastien Ferreira-Cerca, Vatsala Sagar, Thorsten Schäfer, Anne-Maria Wesseling, Eileen Chai,
Ed Hurt and Nicole LaRonde-LeBlanc:
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit.
Nat. Struct. Mol. Biol. 19, 1316-1323 (2012).

Jochen Baßler, Isabella Klein, Claudia Schmidt, Emma Thomson, Martina Kallas, Maria Anna Wagner, Gerald Rechberger, Heimo Strohmaier, Ed Hurt,Helmut Bergler:
The conserved Bud20 zinc finger protein is a new component of the ribosomal 60S
subunit export machinery.
Mol. Cell Biol., 32, 4898-912 (2012).

Divyang Jani, Sheila Lutz, Ed Hurt, Ronald Laskey, Murray Stewart and Vihandha Wickramasinghe:
Functional and structural characterisation of the mammalian TREX-2 complex that links transcription with nuclear mRNA export.
Nucl. Acid Res. 40, 4562-4573 (2012).

Dirk Flemming, Damien Devos, Johannes Schwarz, Stefan Amlacher, Malik Lutzmann and Ed Hurt:
Analysis of the yeast nucleoporin Nup188 reveals a conserved S-like structure with similarity to karyopherins.
J. Struct. Biol. 177, 99-105 (2012).

Andrew M. Ellisdon, Lyudmila Dimitrova, Ed Hurt and Murray Stewart:
Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex.
Nat. Struct. Mol. Biol. 19, 328-336 (2012).

Dieter Kressler, Ed Hurt, Helmut Bergler and Jochen Baßler:The power of AAA- ATPases on the road of pre-60S ribosome maturation - molecular machines that strip pre-ribosomal particles.
Biophys. Acta1823, 92-100 (2012).


Gert Bange, Nico Kümmerer, Przemyslaw Grudnik, Robert Lindner, Georg Petzold, Dieter Kressler, Ed Hurt, Klemens Wild and Irmgard Sinning:
Structural basis for the molecular evolution of SRP-GTPase activation by protein.
Nature Struct. Mol. Biol. 18, 1376-1380 (2012).

Stefan Amlacher, Phillip Sarges, Dirk Flemming, Vera van Noort, Ruth Kunze, Damien Devos, Manimozhiyan Arumugam, Peer Bork and Ed Hurt:
Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile.
Cell 146, 277-289, July 22 (2011).

Susana Rodriguez-Navarro and Ed Hurt:
Linking gene regulation to mRNA production and export.
Curr. Opinion Cell Biol. 23, 302-309 (2011).

Philipp Stelter, Ruth Kunze, Jessica Fischer and Ed Hurt: Probing the FG  repeat network of nucleoporins defines structural and functional features of  the nuclear pore complex
J. Cell Biol. 195, 183-192 (2011)


Dieter Kressler, Ed Hurt and Jochen Bassler:
Driving Ribosome Assembly.
Biochim. Biophys. Acta 1803, 673-683 (2010).

Andrew M. Ellisdon, Divyang Jani, Alwin Köhler, Ed Hurt and Murray Stewart:
Structural basis for the interaction between yeast Spt-Ada-Gcn5 acetyltransferase (SAGA) complex components Sgf11 and Sus1.
J. Biol. Chem. 285, 3850-3856 (2010).

Alwin Köhler and Ed Hurt:
Gene regulation by nucleoporins and links to cancer.
Mol. Cell 38, 6-15 (2010).

Dirk Flemming, Karsten Thierbach, Philipp Stelter, Bettina Boettcher and Ed Hurt:
Precise mapping of subunits in multiprotein complexes by a versatile EM-label.
Nature Struct. Mol. Biol. in press (2010).

Alwin Köhler, Eric Zimmermann, Maren Schneider, Ed Hurt and Ning Zheng:
Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module.
Cell 141, 606-617 (2010).

Jochen Baßler, Martina Kallas, Matthias Thoms, Cornelia Ulbrich, Brigitte Pertschy and Ed Hurt:
The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly.
Mol. Cell in press (2010).

Gunes Bozkurt, Klemens Wild, Stefan Amlacher, Ed Hurt, Bernhard Dobberstein and Irmgard Sinning:
The structure of Get4 reveals an ±-solenoid fold adapted for multiple interaction in tail-anchored protein biogenesis.
FEBS Lett. 584, 1509-1514 (2010).


Andrew M. Ellisdon, Divyang Jani, Alwin Köhler, Ed Hurt and Murray Stewart: Structural basis for the Sus1:Sgf11 interaction in the yeast SAGA transcription activation complex.
J. Biol. Chem. in press (2009).

Gunes Bozkurt, Goran Stjepanovic, Fabio Vilardi, Stefan Amlacher, Klemens Wild, Gert Bange, Vincenzo Favaloro, Karsten Rippe, Ed Hurt, Bernhard Dobberstein and Irmgard Sinning: Structural insights into tail-anchored protein binding and membrane insertion by Get3.
Proc. Natl. Acad. Sci. USA 106, 21131-21136 (2009).

Julien Batisse, Claire Batisse, Aidan Budd, Bettina Böttcher and Ed Hurt: Purification of poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein (mRNP) core structure.
J.Biol.Chem. 284, 354911-354917 (2009).

Brigitte Pertschy, Claudia Schneider, Maren Gnädig, Thorsten Schäfer, David Tollervey and Ed Hurt: RNA helicase Prp43 and its co-factor Pfa1 promote 20S to 18S rRNA processing catalyzed by the endonuclease Nob1.
J.Biol.Chem. 284, 35079-35091 (2009).

Cornelia Ulbrich, Meikel Diepholz, Jochen Baßler, Dieter Kressler, Brigitte Pertschy, Kiki Galani, Bettina Böttcher and Ed Hurt: Mechanochemical Removal of Ribosome Biogenesis Factors from Nascent 60S Ribosomal Subunits.
Cell 138, 911-922 (2009).

Sébastien Ferreira-Cerca and Ed Hurt: Arrest by Ribosome
Nature 459, 46-47 (2009).

Christoph Klöckner, Maren Schneider, Sheila Lutz, Dieter Kressler, Divyang Jani, Murray Stewart, Ed Hurt and Alwin Köhler: Mutational Uncoupling of Sus1's role in NPC-targeting of an mRNA Export Complex and Histone H2B deubiquitination
J. Biol. Chem. 284, 12049-12056 (2009).

Marius Boulos Faza, Stefan Kemmler, Sonia Jimeno, Christina González-Aguilera, Andres Aguilera, Ed Hurt and Vikram Govind Panse: Sem1 is a functional component of the nuclear pore complex associated mRNA export machinery
J. Cell Biol. 184, 833-846 (2009).

Divyang Jani, Sheila Lutz, Neil J. Marshall, Tamas Fischer, Alwin Köhler, Andrew M. Ellisdon, Ed Hurt and Murray Stewart: Sus1, Cdc31 and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export
Mol. Cell 33, 727-737 (2009).

Jun Katahira, Hitomi Inoue, Ed Hurt, Yoshihiro Yoneda: The adaptor Aly and co-adaptor Thoc5 function in Tap-p15 mediated nuclear export of HSP70 mRNA
EMBO J. 28, 556-567 (2009).

Michal Skruzny, Claudia Schneider, Attila Racz, Julan Weng, David Tollervey and Ed Hurt: An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes
PLoS Biology 7 e8 (2009).

Lindsay S. Garrenton, Andreas Braunwarth, Stefan Irniger, Ed Hurt, Markus Künzler and Jeremy Thorner: Nucleus-Specific and Cell Cycle-Regulated Degradation of MAPK Scaffold Protein Ste5 Contributes to the Control of Signaling Competence
Mol. Cell Biol. 29, 582-601 (2009).

Thierry Lacombe, Juan J. García-Gómez, Jesús de la Cruz, Daniela Roser, Ed Hurt, Patrick Linder and Dieter Kressler: Linear ubiquitin fusion to Rps31 and its subsequent cleavage 1 are required for the efficient production and functional integrity of 40S ribosomal subunits
Mol. Microbiol. 72, 69-84 (2009).

Dirk Flemming, Philipp Sarges, Philipp Stelter, Andrea Hellwig, Bettina Boettcher and Ed Hurt: A toxic nucleoporin mutant induces abarrent nucleoporin foci in the cytoplasm
J. Cell Biol. 185, 387-396 (2009).


Ed Hurt: “Der Kernporenkomplex oder das Tor zur Welt des Zytoplasmas” im Jahrbuch 2007 der Deutschen Akademie der Naturforscher Leopoldina.
Leopoldina 53, 457-460 (2008).

Stefanie Grund, Tamas Fischer, Ghislain G. Cabal, Oreto Antúnez, José E. Pérez-Ortín and Ed Hurt: The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
J. Cell Biol. 182, 897-910 (2008).

Rainer Beck, Zhe Sun, Frank Adolf, Chistoph Rutz, Jochen Baßler, Klemens Wild, Irmgard Sinning, Ed Hurt, Britta Brügger, Julien Béthune and Felix Wieland: Membrane curvature induced by GTP-dependent dimerization of Arf1 is essential for vesicle formation
Proc. Natl. Aca. Sci. USA, 105, 11731-11736 (2008).

Dieter Kressler, Daniela Roser, Brigitte Pertschy and Ed Hurt: The AAA-ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles
J. Cell Biol. 181, 835-844 (2008).

Alwin Köhler, Maren Schneider, Ghislain Cabal, Ulf Nehrbass and Ed Hurt: Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export
Nat. Cell Biol. 10, 707-715 (2008).

Nils Schrader, Philipp Stelter, Dirk Flemming, Ruth Kunze, Ed Hurt* and Ingrid Vetter* (*corresponding authors): Structural basis of the Nic96 subcomplex organization in the nuclear pore channel
Mol. Cell 29, 46-55 (2008).

Wei Yao, Malik Lutzmann and Ed Hurt: A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export
EMBO J. 27, 6–16 (2008).



Vikram Govind Panse and Ed Hurt: Adjunct duties for karyopherins: Regulating septin sumoylation
Dev. Cell 12, 669-670 (2007).

Alwin Köhler and Ed Hurt: Exporting RNA from the nucleus to the cytoplasm
Nature Rev. Mol. Cell Biol. 8, 761-773 (2007).

Eva Kowalinski, Gert Bange, Bettina Bradatsch, Ed Hurt, Klemens Wild and Irmi Sinning. The crystal structure of Ebp1 reveals how the methionine
aminopeptidase fold serves as a binding platform for multiple interactions
FEBS Lett. 581, 4450-4454 (2007).

Wei Yao, Daniela Roser, Alwin Köhler, Bettina Bradatsch, Jochen Baßler and Ed Hurt:
Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2
Mol. Cell 26, 51-62 (2007).

Philipp Stelter, Ruth Kunze, Dirk Flemming, Dominic Höpfner, Meikel Diepholz, Peter Philipsen, Bettina Böttcher and Ed Hurt:
Molecular basis for the functional interaction of dynein light chain with the nuclear pore complex
Nature Cell Biology 9, Number 7, 788-796 (2007).

Bettina Bradatsch, Jun Katahira, Eva Kowalinski, Gert Bange, Wei Yao, Toshihiro Sekimoto, Viola Baumgärtel, Guido Boese, Jochen Bassler, Klemens Wild, Reiner Peters, Yoshihiro Yoneda, Irmi Sinning and Ed Hurt: Arx1 functions as alternative nuclear export receptor for the 60S pre-ribosomal subunit
Mol. Cell 27, 767-779 (2007).


Ulrike Hardeland and Ed Hurt:
Coordinated nuclear import of RNA polymerase III subunits
Traffic 7, 465-473 (2006).

Yoshimi Honma, Aiko Kitamura, Ryo Shioda, Hironori Maruyama, Kanako Ozaki, Yoko Oda, Thierry Mini, Paul Jen, Yasushi Maki, Kazuhito Yonezawa, Ed Hurt, Masaru Ueno, Masahiro Uritani, Michael N. Hall, and Takashi Ushimaru: TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients
EMBO J. 25, 3832-3842 (2006).

Jochen Baßler, Martina Kallas and Ed Hurt: The Nug1 GTPase reveals an N-terminal RNA binding domain that is essential for association with pre-60S subunits.
J. Biol. Chem. 281, 24737-24744 (2006).

Vikram Govind Panse, Dieter Kressler, Andrea Pauli, Elisabeth Petfalski, Marén Gnädig, David Tollervey and Ed Hurt: Formation and nuclear export of pre-ribosomes are functionally linked to the small-ubiquitin-related (SUMO) modifier pathway
Traffic 7, 1311-1321 (2006).

Alwin Köhler, Pau Pascual-García, Ana Llopis, Meritxell Zapater, Francesc Posas, Ed Hurt and Susana Rodríguez-Navarro: The mRNA Export Factor Sus1 Is Involved in SAGA-mediated H2B Deubiquitinylation through Its Interaction with Ubp8 and Sgf11
Mol. Biol. Cell. 17, 4228-4236 (2006).

Ghislain Cabal, Susana Rodrigez-Navarro, August Genevesio, Jean-Christophe Olivo-Marin, Christophe Zimmer, Olivier Gadal, Frank Feuerbach-Fournier, Annick Lesne, Henri Buc, Ed Hurt* and Ulf Nehrbass* (*corresponding authors): Molecular Analysis of SAGA Mediated Gene Gating In Yeast
Nature 441, 770-773 (2006).

Thorsten Schäfer, Bohumil Maco, Elisabeth Petfalski,
David Tollervey, Bettina Boettcher, Ueli Aebi and Ed Hurt:
A round of phosphorylation/de-phosphorylation triggers maturation of the pre-ribosomal 40S subunit
Nature 441, 651-655 (2006).


Seiji Masuda, Rita Das, Ed Hurt, Nijsje Dorman and Robin Reed:
Recruitment of the human TREX complex to mRNA during splicing
Genes and Develop.19, 1512-1517 (2005).

Kyriaki Galani, Eduard C. Hurt and George Simos:
The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus
by an Xpo1p-dependent mechanism
FEBS Lett. 579, 969-975 (2005).

Alexander Yarunin, Vikram Panse, Elisabeth Petfalski, Christophe Dez, David Tollervey and Ed Hurt:
Functional link between ribosome formation and biogenesis of iron-sulfur proteins
EMBO J. 24, 580-588 (2005).

Malik Lutzmann, Ruth Kunze, Karin Stangl, Philipp Stelter, Katalin Fejes Tóth, Bettina Boettcher and Ed Hurt:
Reconstitution of Nup157 and Nup145N into the Nup84 complex
J. Biol. Chem. 280, 18442-18451 (2005).


Kyriaki Galani, Tracy A. Nissan, Elisabeth Petfalski, David Tollervey and Ed Hurt:
Rea1, a dynein-related nuclear AAA-type ATPase, is involved in late 25S rRNA processing and nuclear export of 60S subunits.
J. Biol. Chem. 279, 55411-55418 (2004).

Tamas Fischer, Susana Rodriguez-Navarro, Elmar Schiebel and Ed Hurt:
Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery
Nat. Cell Biol. 6, 840—848 (2004).

Vikram Govind Panse, Ulrike Hardeland, Thilo Werner, Bernhard Kuster and Ed Hurt:
A proteome-wide approach identifies sumolyated substrate proteins in yeast
J. Biol. Chem., in press (2004).

Francesca Saracino, Jochen Baßler, Diego Muzzini, Ed Hurt and Maria Luisa Agostoni Carbone:
The Yeast Kinase Swe1 is Required for proper Entry into a new Cell Cycle after Arrest due to
Ribosome Biogenesis Defects
Cell Cycle 3, 648-54 (2004).

Tracy A. Nissan, Kyriaki Galani, Bohumil Maco, David Tollervey, Ueli Aebi and Ed Hurt:
A nascent pre-ribosome with a tadpole-like structure functions in ATP-dependent
maturation of 60S subunits
Mol. Cell 15, 295-301 (2004)

Ed Hurt, Ming-juan Luo, Susanne Röther, Robin Reed and Katja Sträßer:
Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex
PNAS Vol. 101, no. 7, 1858-1862

Susana Rodriguez-Navarro, Tamás Fischer, Ming-Juan Luo, Oreto Antunez, Susanne Brettschneider, Jose E. Perez-Ortin, Robin Reed, and Ed Hurt:
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery
Cell 116, 75-86


Silvia Neumann, Elisabeth Petfalski, Britta Brügger, Helge Großhans, Felix Wieland, David Tollervey and Ed Hurt:
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p.
EMBO Rep. 4, 1156-1162

Thorsten Schäfer, Daniela Strauß, Elisabeth Petfalski, David Tollervey and Ed Hurt:
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes
EMBO J. 22, 1370-1380

Andreas Braunwarth, Michel Fromont-Racine, Pierre Legrain , Ral f Bischoff, Thomas Gerstberger,
Ed Hurt and Markus Künzler:
Identification and characterization of a novel RanGTP binding protein Yrb30
J. Biol. Chem. 278, 15397-15405

Herbert Tschochner and Ed Hurt:
Pre-Ribosomes on the Road from the Nucleolus to the Cytoplasm
Trends Cell Biol. 13, 255-263

Milkereit P., Strauss D., Bassler J., Gadal O., Kühn H., Schütz S., Gas N., Lechner J., Hurt E.,
Tschochner H.:
A Noc Complex Specifically Involved in the Formation and Nuclear Export of Ribosomal 40 S Subunits
The Journal of Biological Chemistry, Vol. 278, No. 6, pp. 4072-4081

Panse V., Küster B., Gerstberger Th., Hurt E.:
Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins
Nature Cell Biology, Vol. 5, 21-27


Fischer T. Strässer K., Rácz A., Rodriguez-Navarro S., Oppizzi M., Ihrig P., Lechner J., Hurt E.:
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores
EMBO J., 21, No. 21:5843-5852

Grandi P., Rybin V., Bassler J., Petfalski E., Strauss D., Marzioch M., Schaefer T., Kuster B., Tschochner H., Tollervey D., Gavin AC., Hurt E.:
90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors.
Mol Cell. 2002 Jul; 10(1):105-15.

Nissan T., Bassler J., Petfalski E., Tollervey D., Hurt E.:
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm.
EMBO J. 2002 Oct 15; 21(20):5539-47

Malik Lutzmann, Ruth Kunze, Andrea Buerer, Ueli Aebi and Ed Hurt:
Modular Self-Assembly of a Y-Shaped Multiprotein Complex from Seven Nucleoporins.
EMBO J., 21, No. 3, 387-397 (2002).

Jun Katahira, Katja Straesser, Takuya Saiwaki, Yoshihiro Yoneda, and Ed Hurt.
Complex Formation between Tap and p15 Affects Binding to FG-repeat Nucleoporins and Nucleocytoplasmic Shuttling
J. Biol. Chem., 11, No. 11, March, 9242-9246 (2002)

Simos G, Grosshans H, Hurt E:
Nuclear export of tRNA
Results Probl. Cell Differ. 35, 115-31 (2002).

Richard Grant , Ed Hurt, David Neuhaus and Murray Stewart:
Structure of the C-terminal FG-Nucleoporin binding domain of Tap/NXF1
Nat. Struct. Biol. 9, 247-251 (2002) (reprints not available)

Susana Rodríguez-Navarro, Katja Sträßer and Ed Hurt:
An intron in the YRA1 gene is required to control Yra1p protein expression and mRNA export
EMBO Rep. 3, 438-442(2002)

Robin Reed and Ed Hurt:
A conserved mRNA export machinery coupled to pre-mRNA splicing
Cell 108, 523-531 (2002)

Katja Sträßer, Seiji Masuda, Paul Mason, Jens Pfannstiel, Marisa Oppizzi, Susana Rodriguez-Navarro, Ana G. Rondón, Andres Aguilera, Kevin Struhl, Robin Reed and Ed Hurt:
TREX is a conserved complex coupling transcription with mRNA export
Nature 417, 304-308 (2002).

Olivier Gadal, Daniela Strauß, Elisabeth Petfalski, Pierre-Emmanuel Gleizes, Nicole Gas, David Tollervey, and Ed Hurt:
Rlp7p is associated with 60S pre-ribosomes, restricted to the granular component of the nucleolus and required for pre-rRNA processing
J. Cell Biol. in press (2002).


Susanne M. Bailer, Carolin Balduf, and Ed Hurt:
The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for
assembly of nucleoporin subcomplexes and nucleocytoplasmic transport
Mol. Cell. Biol., 21, 7944-7955 (2001).

Markus Künzler and Ed Hurt:
Targeting of Ran: variation on a common theme?
J. Cell Sci. 114, 3233-3241 (2001).

Katja Sträßer and Ed Hurt:
The splicing factor Sub2p interacts directly with the mRNA transport factor Yra1p and is required for
nuclear mRNA export
Nature 413, 648-652 (2001).

B. Senger, D. Lafontaine, O. Gadal, J. Graindorge, A. Camasses, A.Sanni, J. Garnier, M. Breitenbach ,
E. Hurt, and F. Fasiolo:
Elongation factor like-1, a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2 is
required for ribosome export and a late cytoplasmic step of ribosomal assembly
Molecular Cell, 8, 1363-1373 (2001).

Jochen Baßler, Paola Grandi, Olivier Gadal, Torben Leßmann, David Tollervey, Johannes Lechner
and Ed Hurt:
Identification of a 60S pre-ribosomal particle that is closely linked to nuclear export
Molecular Cell 8, 517-529 (2001).

K. Galani, H. Großhans, Deinert, K., E. C. Hurt,and G. Simos:
Regulation of intracellular location of aminoacyl-tRNA-synthetases by complex formation with Arc1p
EMBO J. 20, 6889-6898 (2001).

Helge Großhans, Francois Lecointe, Henri Grosjean Ed Hurt, and George Simos:
Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is
compromised in yeast
J. Biol. Chem. 276, 46333-46339 (2001).

Karina Deinert, Franco Fasiolo, Eduard C. Hurt and George Simos:
Arc1p Organizes the Yeast Aminoacyl-tRNA Synthetase Complex and Stabilizes Its Interaction with the
Cognate tRNAs.
J. Biol. Chem. 276, No. 8, February, 6000-6008

Olivier Gadal, Daniela Strauss, Jacques Kessl, Bernard Trumpower, David Tollervey and Ed Hurt:
Nmd3p functions as a nuclear adaptor for Xpo1p-dependent export of 60S ribosomal subunits
Mol. Cell. Biol. 21, 3405-3415 (2001)

Helge Großhans, Karina Deinert, Ed Hurt and George Simos:
Biogenesis of the Signal Recognition Particle Requires Nuclear Import of SRP proteins, Assembly with the
SRP-RNA in the Nucleolus, and Xpo1p-mediated Nuclear Export
J. Cell Biol. 153, 745-62 (2001)

Philipp Milkereit, Olivier Gadal, Alexander Podtelejniko V., Stephanie Trumtel, Nicole Gas, Elisabeth Petfalski, David Tollervey, Mathias Mann, Ed Hurt and Herbert Tschochner:
Maturation of Pre-Ribosomes Requires Noc-Proteins and is Coupled to Transport from the Nucleolus to the Nucleoplasm
Cell 105, 499-509 (2001)

Olivier Gadal, Daniela Strauß, Joris Braspenning, Dominic Hoepfner, Elisabeth Petfalski, Peter Philippsen, David Tollervey and Ed Hurt:
A nuclear AAA-type ATPase (Rix7p) is required for large ribosomal subunit biogenesis and subsequent export to the cytoplasm
EMBO J., 20, 3695-3704 (2001)

Künzler M., Trueheart J., Sette C., Hurt E., Thorner J:
Mutations in the YRB1 Gene Encoding Yeast Ran-Binding-Protein-1 That Impair Nucleocytoplasmic
Transport and Suppress Yeast Mating Defects
Genetics 157, 1089-1105


Zhaolan Zhou, Ming-juan Luo, Katja Sträßer, Jun Katahira, Ed Hurt and Robin Reed:
The protein ALY kinks pre-messenger-RNA splicing to nuclear export in metazoans
Nature, 407, 401-405 (2000)

Kosova, B., N. Panté, C.Rollenhagen, A. Podtelejnikov, M. Mann, U. Aebi and E. Hurt:
Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with Nic96p.
J. Biol. Chem. 275:343-350 (2000)

Grosshans, H., E.C. Hurt and G. Simos:
An aminoacylation-dependent nuclear tRNA export pathway in yeast.
Genes &. Dev. 14, 830-840 (2000)

Ed Hurt, Katja Sträßer, Alexandra Segref, Nick Schlaich, Carlo Presutti, David Tollervey and Ralf Jansen: Mex67p Mediates the Nuclear Export of a Variety of Pol II Transcripts.
J. Biol. Chem. 275, 8361-8368 (2000)

Denise Lau, Markus Künzler, Andreas Braunwarth, Klaus Hellmuth, Alexandre Podtelejnikov, Matthias Mann and Ed Hurt: Purification of ProteinA-Tagged Yeast Ran Reveals Association with a Novel Karyopherin b Family Member Pdr6p.
J. Biol. Chem. 275, 467-471 (2000)

Katja Sträßer and Ed Hurt: Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export.
EMBO J. 19, 410-420 (2000)

Juri Rappsilber, Symeon Siniossoglou, Eduard C. Hurt and Matthias Mann:
A Generic Strategy to Analyze the Spatial Organization of Multi-Protein Complexes by Cross-Linking and
Mass Spectrometry.
Anal Chem.72, 267-75 (2000)

Helge Grosshans, George Simos and Ed Hurt:
Transport of tRNA Out of the Nucleus - Direct Channeling to the Ribosome?
J. Struct. Biol.129, 288-294 (2000)

Markus Künzler, Thomas Gerstberger, Francoise Stutz, and Ed Hurt:
Yeast Ran-binding protein 1 (Yrb1) shuttles between nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway
Mol. Cell Biol. 20, 4295-4308 (2000)

Symeon Siniossoglou, Malik Lutzmann, Helena Santos-Rosa, Kevin Leonard, Shirley Mueller, Ueli Aebi and Ed Hurt: Structure and Assembly of the Nup84p Complex
J. Cell. Biol. 149, 41-53 (2000)

Susanne Bailer, C. Balduf, J. Katahira, A. Podtelejnikov, Ch. Rollenhagen, M. Mann, N. Panté
and Ed Hurt: Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex
J. Biol. Chem. 275, 23540-23548 (2000)

Katja Sträßer, Jochen Bassler and Ed Hurt: Binding of the Mex67p/Mtr2p Heterodimer to FXFG-, GLFG-, and FG-Repeat Nucleoporins is essential for Nuclear mRNA Export
J. Cell Biol., 150, 695-706 (2000)

Birthe Fahrenkrog, John Aris, Ed Hurt, Nelly Panté and Ueli Aebi:
Comparative spatial localization of Protein-A-tagged and authentic yeast nuclear pore complex proteins by immunogold electron microscopy
J. Struct. Biol.129, 295-305 (2000)

Symeon Siniossoglou, Ed Hurt and Hugh R. B. Pelham:
Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast.
J. Biol. Chem. 275, 19352-19360 (2000)

Klaus Hellmuth, Henri Grosjean, Yuri Motorin, Karina Deinert, Ed Hurt and George Simos:
Cloning and characterization of the Schizosaccharomyces pombe tRNA: pseudouridine synthase Pus1p
Nucleic Acids Research, Vol. 28, No. 23, 4604-4610 (2000)

Natalia Gomez-Ospina, Garry Morgan, Thomas H. Giddings Jr., Buket Kosova, Ed Hurt and Mark Winey:
Yeast Nuclear Pore Complex Assembly Defects Determined by Nuclear Envelope Reconstruction
J. Struct. Biol., Vol. 132, No. 1, October 2000, 1-5 (2000)


Hurt, E.C., S. Hannus, B. Schmelzl, D. Lau, D. Tollervey and G. Simos:
A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle
and Nucleoporin Mutants.
J. Cell Biol. 144(3):389-401.

Massenet, S., Y. Motorin, E. Hurt, H. Grosjean and C. Branlant:
Pseudouridine Mapping in the Yeast spliceosomal UsnRNAs Reveals that Pseudouridine Synthase Pus1p
Exhibits a Dual Substrate Specificity for U2snRNA and tRNA.
Mol. Cell. Biol. 19:2142 - 2154.

Katahira, J., K. Sträßer, A. Podtelejnikov, M. Mann, J. U. Jung, and E. C. Hurt:
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human.
EMBO J., 18:2593-2609.

Kosova, B., N. Panté, C. Rollenhagen, and E.C. Hurt:
Nup192p is a Conserved Nucleoporin with a Preferential Location at the Inner Site of the Nuclear Membrane.
J. Biol. Chem. 274:22646 - 22651.

Sträßer, K., and E.C. Hurt: Nuclear export of mRNA in yeast.
FEBS Lett. 452:77 - 81.

Simos, G., and E.C. Hurt: tRNA aminoacylation - a visa to leave the nucleus.
Curr. Biol. 9:R238 - R241.


F. Lecointe, G. Simos, A.Sauer, E.C. Hurt, Y. Motorin, and H. Grosjean:
Characterization of yeast protein Deg1 as Pseudouridine synthase (Pus3) catalyzing the formation of Y38 and Y39 in tRNA anticodon loop.
J. Biol. Chem. 273, 1316-1323 (1998)

S. Siniossoglou, P. Grandi, and E.C. Hurt: Affinity Purification of Protein A-Tagged Nuclear Pore Proteins from Yeast. Cell Biology A Laboratory Hand Book.
Academic Press. Second Edition. Vol. 2, 159 - 164 (1998).

G. Simos, A. Sauer, F. Fasiolo, and E.C. Hurt:
A tRNA-binding domain within Arc1p delivers tRNA to two aminoacyl-tRNA synthetases.
Molecular Cell 1, 235 - 242 (1998).

S. Bailer, S. Siniossoglou, S. Podtelejnikov, A. Hellwig, M. Mann, and E.C. Hurt:
Nup116p and Nup100p are Interchangeable through a Conserved Motif which Constitutes a Binding Site for the mRNA Transport Factor Gle2p/Nup40p.
EMBO J. 17, 1107-1119 (1998).

B. Senger, G. Simos, F.R. Bischoff, A. Podtelejnikov, M. Mann, and E.C. Hurt:
Mtr10p Functions as a Nuclear Import Receptor for the mRNA Binding Protein Npl3p.
EMBO J. 17, 2196-2207 (1998)

M. Künzler and E.C. Hurt:
Cse1p functions as the nuclear export receptor for importin alpha in yeast.
FEBS Lett., 433, 185-190 (1998)

K. Hellmuth, D. Lau, R. Bischoff, M. Künzler, E.C. Hurt, and G. Simos:
Yeast Los1p Has Properties of an Exportin-Like Nucleocytoplasmic Transport Factor for tRNA.
Mol. Cell. Biol. 18(11) 6374-6386 (1998)

H. Santos-Rosa, H. Moreno, G. Simos, A. Segref, B. Fahrenkrog, N. Panté, and E.C. Hurt:
Nuclear mRNA Export Requires Complex Formation between Mex67p and Mtr2p at the Nuclear Pores.
Mol. Cell. Biol. 18(11) 6826-6838 (1998)

B. Fahrenkrog, E.C. Hurt, U. Aebi, and N. Panté:
Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes.
J. Cell Biol. 143, 577 ? 588 (1998).

Y. Motorin, G. Keith, C. Simon, D. Foiret, G. Simos, E. Hurt, and H. Grosjean:
Yeast tRNA: pseudouridine synthase (Pus1p) displays a multisite substrate specificity.
RNA 4, 856 - 869 (1998).

E. Hurt and G. Schütz: Nuclear compartments and nuclear machines.
Curr. Opin. Cell Biol. 10, 301 - 303 (1998).

S. Siniossoglou, H. Santos-Rosa, J. Rappsilber, M. Mann and E. Hurt:
A Novel Complex of Membrane Proteins Required for Formation of a Spherical Nucleus.
EMBO J. 17, 6449-6464 (1998).

E. Hurt: 'Rush Hour' am Zellkern.
Ruperto Carola 1, 12 -17 (1998).


N.L. Schlaich, M. Häner, U. Aebi, and E.C. Hurt:
In Vitro Reconstitution of a Heterotrimeric Nucleoporin Complex Consisting of Recombinant Nsp1p, Nup49p and Nup57p.
Mol. Biol. Cell 8,33-46 (1997)

A. Segref, K. Sharma, V. Doye, A. Hellwig, J. Huber, R. Lührmann, and E.C. Hurt:
Mex67p, A Novel Factor for Nuclear mRNA Export, Binds to Both Poly(A)+ RNA and Nuclear Pores.
EMBO J. 11, 3256 - 3271 (1997).

V. Doye, and E.C. Hurt:
From Nucleoporins to Functional Nuclear Pore Complexes.
Curr. Opin. Cell Biol.9, 401 - 411 (1997)

M.T. Teixeira, S. Siniossoglou, S. Podtelejnikov, J.-C. Binichou, M. Mann, E.C. Hurt, and E. Fabre:
Two Functionally Distinct Domains Generated by in vivo Cleavage of Nup145p: a Novel Biogenesis Pathway for Nucleoporins.
EMBO J. 16, 5086 - 5097 (1997)

P. Grandi, T. Dang, N. Panti, A. Shevchenko, M. Mann, D. Forbes, and E.C. Hurt:
Nup93a, a Vertebrate Homolog of Yeast Nic96p Form a Complex with a Novel 205 kDa Protein and Are
Involved in Nuclear Pore Assembly and Nuclear Growth.
Mol. Biol. Cell. 8, 2017 - 2038(1997)

T. Gautier, T. Bergès, D. Tollervey, and E.C. Hurt:
Nucleolar KKE/D Repeat Proteins Nop56p and Nop58p Interact with Nop1p and Are Required for Ribosome Biogenesis
Mol. Cell. Biol. 17, 7088-7098 (1997).

E. Fabre, and E.C. Hurt:
Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking.
Ann. Rev. Genet. 31, 277-313 (1997).


S. Siniossoglou, C. Wimmer, M. Rieger, V. Doye, H. Tekotte, C. Weise, S. Emig, A. Segref, and E.C. Hurt: A novel complex of nucleoporins, which includes Sec13p and Sec13p homolog, is essential for normal nuclear pores.
Cell 84, 265 - 275 (1996).

K. Sharma, E. Fabre, H. Tekotte, E.C. Hurt, and D. Tollervey:
Yeast nucleoporin mutants are defective in pre-tRNA splicing.
Mol. Cell. Biol. 16, 294-301 (1996).

G. Simos, H. Tekotte, H. Grosjean, A. Segref, K. Sharma, D. Tollervey, and E.C. Hurt:
Nuclear Pore Proteins are Required for the Biogenesis of Functional tRNA.
EMBO J. 15, 2285-2290 (1996).

E.C. Hurt: Importins/karyopherins meet nucleoporins.
Cell 84, 509-515 (1996)

U. Zabel, V. Doye, H. Tekotte, R. Wepf, and E.C. Hurt:
Analysis of Nic96p Reveals that its Central Domain is Required for Nuclear Pore Formation and Together
with its Carboxy-Terminal Domain Functionally Interacts with a Novel Nucleoporin Nup188p.
J. Cell Biol. 133, 1141-1152 (1996)

G. Simos, A. Segref, F. Fasiolo, K. Hellmuth, A. Shevchenko, M. Mann, and E.C. Hurt:
The Yeast Protein Arc1p binds to tRNA and functions as a Cofactor for the Methionyl- and Glutamyl-tRNA
EMBO J. 15, 5437 - 5448 (1996)

G. Vitale, E. Fabre, and E.C. Hurt:
NBP35 encodes an essential and evolutionary conserved protein in Saccharomyces cerevisiae with homology to a superfamily of bacterial ATPases.
Gene 178, 97-106 (1996)

I. Irminger-Finger, E.C. Hurt, A. Roebuck, M. A. Collart, and S.J. Edelstein:
MHP1, an essential gene in Saccharomyces cervisiae required for microtubule function.
J. Cell Biology 135, 1323 - 1340 (1996)


P. Grandi, N.L. Schlaich, H. Tekotte, and E.C. Hurt:
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
EMBO J. 14, 76 - 87 (1995).

N.L. Schlaich, and E.C. Hurt: Disruption of the gene coding for the nuclear pore protein Nup1p leads to nuclear accumulation of poly(A)+ RNA.
Eur. J. Cell Biol. 67, 8 -14 (1995).

V. Doye, and E.C. Hurt:
Genetic approaches to nuclear pore structure and function.
Trends Genet. 11, 193-199 (1995).

P. Grandi, S. Emig, C. Weise, F. Hucho, T. Pohl, and E.C. Hurt:
A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p.
J. Cell Biol. 130, 1263-1273 (1995).

P. Grandi, and E.C. Hurt:
The nuclear pore complex in yeast. In Membrane Protein Transport.
Editor S. S. Rothman, Jai Press Ltd., Vol. 2, 29-56 (1995).

G. Simos, and E.C. Hurt:
Nucleocytoplasmic transport - factors and mechanisms.
FEBS Lett. 369, 107-112 (1995)


E. Fabre, and E.C. Hurt: Nuclear transport.
Curr. Opin. Cell Biol. 6, 335-342 (1994).

T. Berges, E. Petfalski, D. Tollervey, and E.C. Hurt:
Synthetic lethality with fibrillarin idenitifies NOP77p, a nucleolar protein required for pre-rRNA pprocessing and modification.
EMBO J. 13, 3136-3148 (1994).

E. Fabre, W.C. Boelens, C. Wimmer, I. Mattaj, and E.C. Hurt:
Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel
conserved motif.
Cell 78, 275 - 289 (1994).

V. Doye, R. Wepf, and E.C. Hurt:
A novel nuclear pore protein Nup133p with Distinct Roles in poly(A)+ RNA Transport and Nuclear Pore
EMBO J. 13, 6062 - 6075 (1994).


C. Wimmer, V. Doye, U. Nehrbass, N. L. Schlaich, and E.C. Hurt:
Approaches towards a genetic analysis of the nuclear pore complex in yeast.
In 'Protein Synthesis and Targeting' (eds. A.J.P. Brown, M.F. Tuite and J.E.G. McCarthy)
NATO ASI Series, Vol. H 71, pp. 269-281, Springer-Verlag, Berlin-Heidelberg (1993).

D. Tollervey, H. Lehtonen, R. Jansen, H. Kern, and E.C. Hurt:
Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation and ribosome assembly.
Cell 72, 443-457 (1993).

R. P. Jansen, D. Tollervey, and E.C. Hurt: A U3 snoRNP Protein with Homology to Splicing Factor PRP4 and Gb Domains is Required for Ribosomal RNA Processing.
EMBO J. 12, 2549-2558 (1993).

P. Grandi, V. Doye, and E.C. Hurt:
Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96.
EMBO J. 12, 3061-3071 (1993).

E.C. Hurt: The nuclear pore complex.
FEBS Lett. 325, 76-80 (1993).

U. Nehrbass, E. Fabre, S. Dihlmann, W. Herth, and E.C. Hurt:
Analysis of nucleo-cytoplasmic transport in a thermo-sensitive mutant of nuclear pore protein NSP1.
Eur. J. Cell Biol. 62, 1-12 (1993).

G. Schlenstedt, E.C. Hurt, V. Doye, and P.A. Silver:
Reconstitution of nuclear protein transport with semi-intact yeast cells.
J. Cell Biol. 123, 785-798 (1993).


A. Mutvei, S. Dihlmann, W. Herth, and E.C. Hurt:
Depletion of the nucleoporin NSP1 in yeast inhibits nuclear pore formation and nuclear transport of a nuclear reporter protein.
Eur. J. Biol. 59, 280-295 (1992).

C. Wimmer, V. Doye, P. Grandi, U. Nehrbass, and E.C. Hurt:
A New Subclass of Nucleoporins that Functionally Interacts with Nuclear Pore Protein NSP1.
EMBO J. 11, 5051-5061 (1992).

E.C. Hurt, A. Mutvei, and M. Carmo-Fonseca:
The nuclear envelope of the yeast Saccharomyces cerevisiae.
Int. Rev. Cytol. 136, 145-184 (1992).

U. Nehrbass, and E. C. Hurt:
Nuclear transport and nuclear pores in yeast.
Int. J. Gen. Mol. Microbiol. 62, 3-14 (1992).


R.P. Jansen, E.C. Hurt, H. Kern, H. Lehtonen, M. Carmo-Fonseca, B. Lapeyere, and D. Tollervey:
Evolutionary conservation of the human protein fibrillarin and its functional expression in yeast.
J. Cell Biol. 113, 715-727 (1991).

D. Tollervey, H. Lehtonen, M. Carmo-Fonseca, and E.C. Hurt:
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast.
EMBO J. 10, 573-583 (1991).

M. Carmo-Fonseca, D. Tollervey, R. Pepperkok, S.M.L. Barabino, A. Merdes, C. Brunner, P.D. Zamore, M.R. Green, E.C. Hurt, and A.I. Lamond:
Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing
EMBO J. 10, 195-206 (1991).

M. Carmo-Fonseca, H. Kern and E.C. Hurt:
Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization.
Eur. J. Cell Biol. 55, 17-30 (1991).

E.C. Hurt and M. Carmo-Fonseca:
Across the nuclear pores with the help of nucleoporins.
Chromosoma 101, 199-205 (1991).


U. Nehrbass, H. Kern, A. Mutvei, H. Horstmann, B. Marshallsay, and E. C. Hurt:
NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its
carboxy-terminal domain.
Cell 61, 979-989 (1990).

D. Tollervey, and E. C. Hurt:
The role of small nucleolar ribonucleoproteins in ribosome biogenesis.
Mol. Biol. Report 14, 103-206 (1990).

E.C. Hurt: Targeting of a cytosolic protein to the nuclear periphery.
J. Cell Biol. 111, 2829-2837 (1990).


T. Schimmang and E. C. Hurt:
A 40 kDa nuclear membrane protein changes its concentration and localization within the nuclear envelope of the yeast Saccharomyces cerevisiae in a cell cycle dependent manner.
Eur. J. Cell Biol. 49, 33-41(1989).

E. C. Hurt:
NSP1, a yeast protein located at the nuclear periphery is required for the cell-cycle of Saccharomyces
J. Cell Sci. 12, 243-252 (1989).

T. Schimmang, D. Tollervey, H. Kern, R. Frank and E. C. Hurt:
A yeast nucleolar protein related to human fibrillarin is associated with small nucleolar RNA
and essential for viability.
EMBO J. 8, 4015-4025 (1989).


E.C. Hurt, A. McDowell, and T. Schimmang:
Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae.
Eur. J. Cell Biol. 46, 554-563 (1988).

E.C. Hurt:
A novel nucleosceletal-like protein located at the nuclear periphery is required for the life cycle of
Saccharomyces cerevisiae.
EMBO J. 7, 4323-4334 (1988)