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Thomas Söllner 
Regulated Membrane Fusion: Molecular Mechanisms and Machinery

Groupleader: Thomas Söllner

Söllner, T. Malsam, J. (2018) Uncovering the “secret” Lives of Vacuolar Fusion pores in living cells. EMBOJ 37 (19) DOI:10.15252/embj.2018100656.

Meijer M., Dörr B., Lammertse H., Blithikioti C., van Weering J., Toonen R., Söllner T.H., Verhage M. (2018) Tyrosine phosphorylation of Munc18-1 inhibits synaptic transmission by preventin SNARE assembly.
The EMBO Journal 37(2): 300-320

Weber P., Batoulis H., Rink K.M., Dahlhoff S., Pinkwart K., Söllner T.H., Lang T. (2017) Electrostatic anchoring precedes stable membrane attachment of SNAP25/SNAP23 to the plasma membrane.
eLife 6:e19394. DOI: 10.7554/eLife.19394

Schupp M, Malsam J, Ruiter M, Scheutzow A, Wierda KD, Söllner TH, Sørensen JB.
Interactions Between SNAP-25 and Synaptotagmin-1 Are Involved in Vesicle Priming, Clamping Spontaneous and Stimulating Evoked Neurotransmission.
J Neurosci. 2016; 36(47):11865-11880

Munch, A.S., Kedar, G.H., van Weering, J.R.T., Vazquez-Sanchez, S., He, E., Andre, T., Braun, T., Söllner, T.H., Verhage, M., Sørensen, J.B. (2016) Extension of Helix 12 in Munc18-1 Induces Vesicle Priming. J. Neurosci. 36(26): 6881-6891.

Kedar, G.H., Munch, A.S., van Weering, J.R.T., Malsam, J., Scheutzow, A., de Wit, H., Houy, S., Tawfik, B., Söllner, T.H., Sørensen, J.B., Verhage, M. (2015) A post-docking role of synaptotagmin 1-C2B domain bottom residues R398/399 in mouse chromaffin cells. J. Neurosci. 35(42): 14172-14182.

Parisotto, D., Pfau, M., Scheutzow, A., Wild, K., Mayer, M.P., Malsam, J., Sinning, I., Söllner, T.H. (2014) An Extended Helical Conformation in Domain 3a of Munc18-1 Provides a Template for SNARE (Soluble N-Ethylmaleimide-sensitive Factor Attchment Protein Receptor) Complex Assembly. J.Biol.Chem. 289, 9639-9650.

Bharat, T.A., Malsam, J., Hagen, W.J., Scheutzow, A., Söllner, T.H., Briggs, J.A. (2014) SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calsium-triggered fusion. EMBO reports 15:3, 308-314.

Malsam, J., Parisotto, D., Bharat, T.A., Scheutzow A., Krause, J.M., Briggs, J.A., Söllner, T.H. (2012) Complexin arrests a pool of docked vesicles for fast Ca (2+)-dependent release. EMBO J. 31, 3270-3281

Parisotto, D., Malsam, J., Scheutzow, A., Krause, J.M., Söllner, T.H. (2012) SNAREpin Assembly by Munc18-1 Requires Previous Vesicle Docking by Synaptotagmin 1. J. Biol. Chem. 287, 31041-31049

Schollmeier, Y., Krause, J. M., Kreye, S., Malsam, J., Söllner, T.H. (2011). Resolving the Function of Distinct Munc18-1/SNARE Protein Interaction Modes in a Reconstituted Membrane Fusion. JBC 286 No. 35, 30582-30590.

Malsam, J., and Söllner, T.H. (2011). Organization of SNAREs within the Golgi stack. in ‘The Golgi’, Coldspring Harbor Perspectives in Biology (editors Warren, G. and Rothman, J.) Cold Spring Harbor Laboratory Press

Kögel, T., Rudolf, R., Hodneland, E., Hellwig, A., Kuznetsov, S., Seiler, F., Söllner, T.H., Barroso, J., Gerdes, H.-H. (2010). Distinct Roles of Myosin Va in Membrane Remodeling and Exocytosis of Secretory Granules. Traffic, 11: 637-650.

Seiler, F., Malsam, J., Krause, JM., Söllner, T.H. (2009). A role of complexin-lipid interactions in membrane fusion. FEBS letters, 583: 2343-8.

Malsam, J., Seiler, F., Schollmeier, Y., Rusu, P., Krause, JM., Söllner, T.H. (2009). The carboxy-terminal domain of complexin I stimulates liposome fusion. Proc. Natl. Acad. Sci. USA, 106: 2001-2006.

Malsam, J., Kreye, S., Söllner, T.H. (2008). Membrane fusion: SNAREs and regulation. Cell Mol. Life. Sci., 65: 2814-2832.

Kreye, S., Malsam, J., Söllner, T.H. (2008). In vitro assays to measure SNARE mediated membrane fusion. Methods in Mol. Biol. 440: 37-50.

Moelleken, J., Malsam, J., Betts, M.J., Movafeghi, A., Reckmann, I., Meissner, I., Hellwig, A., Russell, R.B., Söllner, T.H., Brügger, B., Wieland, F.T. (2007). Differential localization of isoformic coatomer complexes within the Golgi apparatus. Proc. Natl. Acad. Sci. USA, 104: 4425-30.

Söllner, T.H. (2007). Membrane trafficking. FEBS Letters, 581: 2075.

Söllner, T.H. (2007). Lipid droplets highjack SNAREs. Nat. Cell Biol., 9:1219-20.

Cosson, P., Ravazzola, M., Varlamov, O., Söllner, T.H., Di Liberto, M., Volchuk, A., Rothman J.E., Orci, L. (2005). Dynamic transport of SNARE proteins in the Golgi apparatus. Proc Natl Acad Sci U S A, 102:14647-52.

Cheng, Y. Sequeira, S.M., Malinina, L., Tereshko, V., Söllner, T.H., and Patel, D.J. (2004). Crystallographic identification of Ca2+ and Sr2+ coordination sites in synaptotagmin I C2B domain. Protein Science, 13: 2665-2672.

Söllner, T.H. (2004) Intracellular and viral membrane fusion: a uniting mechanism. Curr. Opinion Cell Biol, 16: 429-435.

Fukasawa, M., Varlamov, O., Eng, W.S., Söllner, T.H., and Rothman, J.E. (2004). Localization and activity of the SNARE Ykt6 determined by its regulatory domain and palmitoylation. Proc. Natl. Acad. Sci. USA, 101: 4815-1820.

Varlamov, O., Volchuk, A., Rahimian, V., Doege, C.A. Paumet, F., Eng, W.S, Arango, N.C., Parlati, F., Ravazzola, M., Orci, L, Söllner, T.H., and Rothman, J.E. (2004). i-SNAREs, inhibitory SNAREs that fine-tune the specificity of membrane fusion. J. Cell Biol., 164: 79-88.

Söllner, T.H., and Sequeira, S. (2003) S-nitrosylation of NSF controls membrane trafficking. Cell, 115: 127-129.

Burri, L., Varlamov, O., Doege, C., Hofman, K., Beilharz, T., Rothman, J.E., Söllner, T.H., and Lithgow, T. (2003). A SNARE required for retrograde transport to the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA, 100: 9873-9877.

Hu, C., Ahmed, M., Melia, T.J., Söllner, T.H., Mayer, T., and Rothman, J.E. (2003). Fusion of cells by flipped SNAREs. Science, 300: 1745-1749.

Söllner T.H. (2003). Regulated exocytosis and SNARE function. Mol. Memb. Biol., 20: 209-220.

Mahal, L.K., Sequeira, S.M., Gureasko, J., and Söllner, T.H. (2002). Calcium-independent stimulation of membrane fusion and SNAREpin formation by synaptotagmin I. J.Cell Biol., 158: 273-282.

Parlati, F., Varlamov, O., Paz, K., McNew, J.A., Hurtado, D., Söllner, T.H., and Rothman, J.E. (2002). Distinct SNARE complexes mediating membrane fusion in Golgi
transport based on combinatorial specificity. Proc. Natl. Acad. Sci. USA, 99: 5424-5429.

Melia, T.J., Weber, T., McNew, J.A., Fisher, L.E., Johnston, R.J., Parlati, F., Mahal, L.K. Söllner, T.H., and Rothman, J.E. (2002). Regulation of membrane fusion by the membrane-proximal coil of the t SNARE during zippering of SNAREpins. J. Cell Biol, 158: 929-940.

McNew, J.A., Parlati, F., Fukuda, R., Johnston, R.J., Paz, K., Paumet, F., Söllner, T.H., and Rothman, J.E. (2000). Compartmental specificity of cellular membrane fusion encoded in SNARE proteins. Nature, 407: 153-159.

Parlati, F., McNew, J.E., Fukuda, R., Miller, R., Söllner, T.H., and Rothman, J.E. (2000). Topological restriction of SNARE-dependent membrane fusion. Nature, 407: 194-198.

Fukuda, R., McNew, J.A., Weber, T., Parlati, F., Engel, T., Nickel, W., Rothman, J.E., and Söllner, T.H. (2000) Functional architecture of an intracellular membrane t-SNARE. Nature, 407: 198-202.

Brügger, B., Nickel, W., Weber, T., Parlati, F., McNew, J.A., Rothman, J.E. and Söllner, T. (2000). Putative fusogenic activity of NSF is restricted to a lipid mixture whose coalescence is also triggered by other factors. EMBO J., 19: 1272-1278.

McNew, J.A., Weber, T., Parlati, F., Johnston, R., Melia, T.J., Söllner, T.H., and Rothman, J.E. (2000). Close is not enough: SNARE-dependent membrane fusion requires an active mechanism that transduces force to membrane anchors. J.Cell Biol., 150: 105-117.

Weber, T., Parlati, F., McNew, J.A., Johnston, R.J., Westermann, B., Söllner, T.H., and Rothman, J.E. (2000). SNAREpins are functionally resistant to disruption by NSF and aSNAP. J.Cell Biol., 149: 1063-1072.

Hohl., T.M., Parlati, F., Wimmer, C., Rothman, J.E., Söllner, T.H., and Engelhardt, H. (1998). Arrangement of subunits in 20 S particles consisting of NSF, SNAPs, and SNARE complexes. Mol. Cell, 2: 539-548.

Weber, T., Zemelman, B.V., McNew, J.A., Westermann, B., Gmachl, M., Parlati, F., Söllner, T.H., and Rothman, J.E. (1998). SNAREpins: minimal machinery for membrane fusion. Cell, 92: 759-772.

Rothman, J.E., and Söllner, T.H. (1997). Throttles and Dampers: controlling the engine of membrane fusion. Science, 276: 1212-1213.

Nagahama, M., Orci, L., Ravazzola, M., Amherdt, M., Lacomis, L., Tempst, P., Rothman, J.E., and Söllner, T.H. (1996). A v-SNARE implicated in intra-Golgi transport. J. Cell. Biol, 133: 507-516

Søgaard, M., Tani, K., Ye, R.R., Geromanos, S., Tempst, T., Kirchausen, T., Rothman, J.E., and Söllner, T. (1994). A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell, 78: 937-948.

Söllner, T., Bennett, M.K., Whiteheart, S.W., Scheller, R.H., and Rothman, J.E. (1993). A protein assembly - disassembly pathway that may correspond to sequential steps of synaptic vesicle docking, activation and fusion. Cell, 75: 409-418.

Söllner, T., Whiteheart, S.W., Brunner, M., Erdjument-Bromage, H., Geromanos, S., Tempst, P., and Rothman, J.E. (1993). SNAP receptors implicated in vesicle targeting and fusion. Nature, 362: 318-324.