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Groupleader: Mandy Jeske

Molecular Functions of Germline Proteins 

Group Leader Mandy Jeske

Mandy Jeske

Molecular Functions of Germline Proteins

  • Research
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Research 


1. Research group April 2022 
Research group April 2022
2. RNA helicases 
RNA helicases
3. Protein crystal 
Protein crystal
4. Electron density map 
Electron density map
5. Drosophila egg chambers with Vasa staining 
Drosophila egg chambers with Vasa staining


In the lab, we are using a multidisciplinary approach to investigate proteins and protein complexes with essential functions during germ cell formation and early embryonic development of Drosophila. The research focusses on understanding molecular mechanism underlying piRNA biogenesis and piRNA-mediated transposon silencing of transposon mRNAs, processes which are highly critical during gametogenesis and early animal development.

In particular, we are currently focusing our studies on LOTUS domain-containing proteins (Kubíková et al., 2020) and on ATP-dependent RNA helicases. To unravel the molecular mechanisms of how these proteins function in the piRNA pathway, we combine RNA/protein biochemistry, X-ray crystallography, and cell culture techniques with Drosophila genetics.

Twitter @Jeske_Lab

CV 


1998 - 2005  Biochemistry Studies at University Halle-Wittenberg
2005 - 2010  PhD at the University Halle-Wittenberg with Elmar Wahle
2010 - 2017
 
 Postdoc at the EMBL with Anne Ephrussi and Christoph W. Müller
 (EIPOD Developmental/Structural biology)
since 2017  Emmy Noether Group Leader at the Heidelberg University Biochemistry Center (BZH)
   

Lab Members 



Asen Garbelyanski
PhD student

Lab:Room 422 / Phone: +49 6221 54-4739
Mail:asen.garbelyanski@bzh.uni-heidelberg.de

 RoomPhone
LAB 422 +49 6221 54-4739
E-Mail asen.garbelyanski@bzh.uni-heidelberg.de
Jana Kubikova
PhD student

Lab:Room 422 / Phone: +49 6221 54-4739
Mail:jana.kubikova@bzh.uni-heidelberg.de

 RoomPhone
LAB 422 +49 6221 54-4739
E-Mail jana.kubikova@bzh.uni-heidelberg.de
Jutta Metz
TA

Lab:Room 422 / Phone: +49 6221 54-6894
Mail:jutta.metz@bzh.uni-heidelberg.de

 RoomPhone
LAB 422 +49 6221 54-6894
E-Mail jutta.metz@bzh.uni-heidelberg.de
Harpreet Kaur Salgania
PhD student

Lab:Room 422 / Phone: +49 6221 54-6894
Mail:harpreet.salgania@bzh.uni-heidelberg.de

 RoomPhone
LAB 422 +49 6221 54-6894
E-Mail harpreet.salgania@bzh.uni-heidelberg.de

Publications 


RNA binding proteins Smaug and Cup induce CCR4–NOT-dependent deadenylation of the nanos mRNA in a reconstituted system. Nucleic Acids Research, published online 23 March 2023, https://doi.org/10.1093/nar/gkad159
 
Pekovic, F., Rammelt, C., Kubíková, J., Metz, J., Jeske, M., Wahle, E. (2023)

Structural basis for binding of Smaug to the GPCR Smoothened and to the germline inducer Oskar. bioRxiv2023.02.19.529116
 
Kubíková, J., Ubartaitė, G., Metz, J., Jeske, M. (2023)

ReLo: a simple colocalization assay to identify and characterize physical protein-protein interactions. BioRxiv. doi: https://doi.org/10.1101/2022.03.04.482790
 
Salgania, H.K., Metz, J., and Jeske, M. (2022)

RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system. BioRxiv. doi:https://doi.org/10.1101/2022.05.11.491288
 
Pekovic, F., Rammelt, C., Kubíková, J., Metz, J., Jeske, M., Wahle, E. (2022)

Molekulare Funktionen von Keimbahn-spezifischen Proteinen. BIOspektrum. 27, 101.
Jeske, M. (2021)

Molecular functions of germline proteins. EU Research. SPR21, 18.
Jeske, M. (2021)

LOTUS-domain proteins - Developmental effectors from a molecular perspective. Biol. Chem. 402, 7–23.
Kubíková, J., Reinig, R., Salgania, H.K., and Jeske, M. (2020)

The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage. Genes Dev. 31(9), 939-952.
Jeske, M., Müller, C.W., Ephrussi, A. (2017)

The crystal structure of the Drosophila germline-inducer Oskar identifies two domains with distinct Vasa helicase- and RNA-binding activities. Cell Rep. 12(4), 587-98.
Jeske, M., Bordi, M., Glatt, S., Müller, S., Rybin, V., Müller, C.W., Ephrussi, A. (2015)

Assaying mRNA deadenylation in vitro. Methods Mol. Biol. 1125, 297–311.
Jeske, M., Temme, C., Wahle, E. (2014)

Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J. Biol. Chem. 288(4), 2441-2451.
Buschmann, J., Moritz, B., Jeske, M., Lilie, H., Schierhorn, A., Wahle, E. (2013)

Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. EMBO J. 30(1), 90-103.
Jeske, M., Moritz, B., Anders, A., Wahle, E. (2011)

Cell-free deadenylation assays with Drosophila embryo extracts. Meth. Enzymol. 448, 107-118.
Jeske, M., Wahle, E. (2008)

Rapid ATP-dependent deadenylation of nanos mRNA in a cell-free system from Drosophila embryos. J. Biol. Chem. 281(35), 25124-25133.
Jeske, M., Meyer, S., Temme, C., Freudenreich, D., Wahle, E. (2006)

Funding 


Open Positions 


Interested Trainees and highly motivated Bachelor and Master students are always welcome to join our lab. Please contact me by mail: jeske@bzh.uni-heidelberg.de

 

Contact



Heidelberg University
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg

Office:
+49 6221 54-4728
Fax:
+49 6221 54-4369
E-Mail:
jeske@bzh.uni-heidelberg.de


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  • Brügger
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  • Schuck
  • Sinning
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  • Former Research Groups »
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