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Senior Professor: Ed Hurt
Ed Hurt
Nuclear pore complex and ribosome assembly
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Research
Nuclear pore complex and ribosome assembly
Ongoing projects
1.Structure and assembly of the nuclear pore complexNuclear pore complexes are huge transport machines that span the double nuclear membrane and mediate nucleocytoplasmic traffic. About 60 MDa in size (in yeast), each NPC consists of 30 subunits (nucleoporins), which assemble into an eight-fold symmetrical complex with distinct substructures. To date, little is known about how this huge complex assembles, and which nucleoporins constitute the distinct substructures. We have chosen a reconstitution approach to study the structure-function relationships of the NPC in detail. A detailed structural analysis of the reconstituted NPC will be crucial to understand how nucleocytoplasmic transport through the nuclear pore complex occurs at the atomic level.
2. Formation and nuclear export of ribosomes
Ribosome formation is a complicated and highly regulated multistep process, which requires more than 150 conserved, mostly essential non-ribosomal factors (e.g. helicases, rRNA processing factors, ATPases, GTPases, Kinases, export factors), which transiently associate with the evolving pre-ribosomal particles. Our studies focus on late steps of ribosome formation and the coupling to nuclear export.
3. Connections between transcription and mRNA export
Gene expression requires bi-directional transport of macromolecules across the nuclear membrane. Among the many components that are exported from the nucleus are messenger RNAs, which are transcribed as pre-mRNAs in the nucleus, followed by RNA processing and modification, RNP assembly and nuclear export. Our studies revealed that the TREX complex, which contains components involved in transcription elongation (THO complex members) and mRNA export (Sub2, Yra1), acts as a coupling device in transcription-coupled mRNA export. Another complex, which contains transcription and export factors (termed TREX-2) also functions in transcription-coupled mRNA export. In this project, we analyze how the nuclear pore-associated TREX-2 mRNA export complex is assembled and bound to the NPC (in collaboration with Murray Stewart, MRC Cambridge, England). Tethering of genes to the nuclear periphery could ultimately bring nascent transcripts into the vicinity of the nuclear pore-associated mRNA export machinery.
Lab Members
This is my Team
Ingo Amm
staff scientist
staff scientist
Lab:Room 423
/ Phone: +49 6221 54-4169
Mail:ingo.amm@bzh.uni-heidelberg.de
Jochen Baßler
staff scientist
staff scientist
Lab:Room 424
/ Phone: +49 6221 54-6763
Office:Room 407
/ Phone: +49 6221 54-6757
Mail:jochen.bassler@bzh.uni-heidelberg.de
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Selene Cordeiro
service
service
Lab:Room 446
/ Phone: +49 6221 54-5422
Mail:selene.cordeiro@bzh.uni-heidelberg.de
Paulina Fischer
PhD student
PhD student
Lab:Room 425
/ Phone: +49 6221 54-4176
Mail:paulina.fischer@bzh.uni-heidelberg.de
Sabine Griesel
TA
TA
Lab:Room 425
/ Phone: +49 6221 54-4176
Mail:sabine.griesel@bzh.uni-heidelberg.de
Ed Hurt
Senior Professor
Senior Professor
Office:Room 409
/ Phone: +49 6221 54-4173
Mail:ed.hurt@bzh.uni-heidelberg.de
Benjamin Lau
postdoc
postdoc
Lab:Room 425
/ Phone: +49 6221 54-4176
Mail:benjamin.lau@bzh.uni-heidelberg.de
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Andrea Schliwa
secretary
secretary
Office:Room 408
/ Phone: +49 6221 54-4183
Mail:andrea.schliwa@bzh.uni-heidelberg.de
CV
Date of birth: January 7th, 1955
Academic Training and Positions
| 1974 - 1979 | Study of biology and chemistry at the University of Regensburg |
| 1980 - 1984 | PhD Student at the University of Regensburg |
| 1984 - 1986 | Postdoc with Prof. Dr. G. Schatz, Biocenter, Basel, Switzland |
| 1986 - 1994 | Group Leader at the EMBL Heidelberg in the Cell Biology |
| 11.07.1990 | Habilitation in Biochemistry at the University of Regensburg |
| 01.03.1994 | Election as member of EMBO |
| since 01/1995 | C4 Professor at Heidelberg University |
| 2003 - 2005 | Director of the Heidelberg University Biochemistry Center (BZH) |
| 2005 | Election as member of Deutsche Akademie der Naturforscher Leopoldina |
| 2007 | Election as member of Academia Europaea |
Publications
Structural inventory of cotranslational protein folding by the eukaryotic RAC complex, bioRxiv https://www.biorxiv.org/content/10.1101/2022.06.24.497458v1, doi: https://doi.org/10.1101/2022.06.24.497458.
Kišonaitė, M., Wild, K., Lapouge, K., Valentín Gesé, G., Kellner, N., Hurt, E. & Sinning, I. (2022)
Global Transcriptome Characterization and Assembly of the Thermophilic Ascomycete Chaetomium thermophilum. Genes 12, 1549. https://doi.org/10.3390/genes12101549 Singh, A., Schermann, G., Reislöhner, S., Kellner, N., Hurt, E., & Brunner, M. (2021)
90S pre-ribosome transformation into the primordial 40S subunit.
Reprint
Science369, 1470-1476.
Cheng J, Lau B, La Venuta G, Ameismeier M, Berninghausen O, Hurt E, Beckmann R. (2020)
Construction of the Central Protuberance and L1 stalk during 60S subunit biogenesis Mol. Cell, doi.org/10.1016/j.molcel.2020.06.032.
Lukas Kater, Valentin Mitterer, Matthias Thoms, Jingdong Cheng, Otto Berninghausen, Roland Beckmann and Ed Hurt (2020)
Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome. Mol. Cell .
Benjamin Lau, Jingdong Cheng, Dirk Flemming, Giusseppe La Venuta, Otto Berninghausen, Roland Beckmann and Ed Hurt (2020)
Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes. Methods in Mol. Biol., in press.
Nikola Kellner and Ed Hurt (2020)
Crystal structures of Rea1-MIDAS bound to its ribosome assembly factor ligands resembling integrin–ligand-type complexes. Nat Commun 10, 3050. https://doi.org/10.1038/s41467-019-10922-6 Ahmed, Y.L., Thoms, M., Mitterer, V., Sinning, I., & Hurt, E. (2019)
Eucaryotic Ribosome Assembly. Annu Rev Biochem. 2019 Jun 20;88:281-306. doi: 10.1146/annurev-biochem-013118-110817.
Baßler J, Hurt E. (2019)
Nucleoporin Nup155 is part of the p53 network in liver cancer. Mol Cell. 2019 Sep 19;75(6):1256-1269.e7. doi: 10.1016/j.molcel.2019.06.032.
Holzer K, Ori A, Cooke A, Dauch D, Drucker E, Riemenschneider P, Andres-Pons A, DiGuilio AL, Mackmull MT, Baßler J, Roessler S, Breuhahn K, Zender L, Glavy JS, Dombrowski F, Hurt E, Schirmacher P, Beck M, Singer S (2019)
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. Mol Cell. 2019 Sep 19;75(6):1256-1269.e7. doi: 10.1016/j.molcel.2019.06.032.
Cheng J, Baßler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E. (2019)
Assembly Kinetics of Vimentin Tetramers to Unit-Length Filaments: A Stopped-Flow Study. Biophys J. 2018 May 22;114(10):2408-2418. doi: 10.1016/j.bpj.2018.04.032.
N. Mücke, L. Kämmerer, S. Winheim, R. Kirmse, J. Krieger, M. Mildenberger, J. Baßler, E. Hurt, WH Goldmann, U. Aebi, K. Toth, J. Langowski, H. Hermann (2018)
Structure of the nuclear exosome captured on a maturing preribosome. Science. 2018 Apr 13;360(6385):219-222. doi: 10.1126/science.aar5428.
Jan Schuller, Sebastian Falk, Lisa Fromm, Ed Hurt and Elena Conti (2018)
Suppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation. Nat Commun. 2018 Oct 5;9(1):4094. doi: 10.1038/s41467-018-06660-w.
Matthias Thoms, Valentin Mitterer, Lukas Kater, L. Falquet, Roland Beckmann, Dieter Kressler, Ed Hurt (2018)
3.2 Angstrœm resolution structure of the 90S pre-ribosome before A1 pre-rRNA cleavage. Nat. Struct. Mol. Biol. doi:10.1038/nsmb.3476.
Jingdong Cheng, Nikola Kellner, Otto Berninghausen, Ed Hurt and Roland Beckmann (2017)
A puzzle of life: crafting ribosomal subunits Trends Biochem. Sci. , in press.
Dieter Kressler, Ed Hurt and Jochen Bassler (2017)
Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae. eLife, 6, e30189. https://doi.org/10.7554/eLife.30189 André Heuer, Emma Thomson, Christian Schmidt, Otto Berninghausen, Thomas Becker, Ed Hurt and Roland Beckmann (2017)
Eukaryotic ribosome assembly, transport and quality control. Nat. Struct. Mol. Biol. 24, 689-699. doi: 10.1038/nsmb.3454.
Cohue Peña, Ed Hurt and Vikram Govind Panse (2017)
Interaction network of the ribosome assembly machinery from a eukaryotic thermophile. Protein science 26(2), 327–342. https://doi.org/10.1002/pro.3085 Baßler, J., Ahmed, Y.L., Kallas, M., Kornprobst, M., Rodriguez-Calvino, F., Gnädig, M., Thoms, M., Stier, G., Ismail, S., Kharde, S., Castillo, N., Griesel, S., Bastuck, S., Bradatsch, B., Thomson, E., Flemming, D., Sinning, I.* & Hurt, E.* (2017)
Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly. Nature Commun., in press.
Miriam Sturm, Jingdong Cheng, Jochen Bassler, Roland Beckmann and Ed Hurt (2017)
Mpp10 represents a platform for the interaction of multiple factors within the 90S pre-ribosome, PlosOne 12: e0183272. Sá-Moura, B., Kornprobst, M., Kharde, S., Ahmed, Y.L., Stier, G., Kunze, R., Sinning*, I. & Hurt*, E. (2017)
Pre-ribosomes escaping the nucleus are caught during translation by cytoplasmic quality control quality control mechanisms. Nat. Struct. Mol. Biol., doi: 10.1038/nsmb.349.
Anshuk Sarkar, Matthias Thoms, Clara Barrio-Garcia, Emma Thomson, Dirk Flemming, Roland Beckmann and Ed Hurt (2017)
Reconstitution of the complete pathway of of ITS2 processing at the pre-ribosome. Nature Commun., in press.
Lisa Fromm, Sebastian Falk, Dirk Flemming, Jan Schuller, Matthias Thoms, Elena Conti and Ed Hurt (2017)
Short linear motifs in scaffold Nups act as sensors to cooperatively connect pre-assembled modules during NPC biogenesis. Nature Commun. 8, 1107. doi: 10.1038.
Roman Teimer, Jan Kosinski, Alexander von Appen, Martin Beck and Ed Hurt (2017)
Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis. PLOS ONE 12(6): e0178752. https://doi.org/10.1371/journal.pone.0178752 Calviño, F.R., Kornprobst, M., Schermann, G., Birkle, F., Wild, K., Fischer, T., Hurt, E., Ahmed, Y.L. & Sinning, I. (2017)
Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the pre-ribosomal protein Nop53. RNA 23, 1780-1787. doi: 10.1261/rna.062901.117.
Sebastian Falk, Jan-Niklas Tants, Ed Hurt, Michael Sattler and Elena Conti (2017)
The nuclear pore complex: understanding its function through structural insight. Nature Rev. Mol. Cell Biol. 18, 73-89.
Martin Beck and Ed Hurt (2017)
Visualizing the assembly pathway of nucleolar pre-60S ribosomes, Cell 171: 1599-1610. Kater, L., Thoms, M., Barrio Garcia, C., Cheng, J., Ismail, S., Ahmed, Y.L., Bange, G., Kressler, D., Berninghausen, O., Sinning, I., Hurt, E. & Beckmann, R. (2017)
Visualizing the earliest steps during nucleolar pre-60S ribosome assembly. Cell 171, 1599–1610. Lukas Kater, Matthias Thoms, Clara Barrio Garcia, Jingdong Cheng, Sherif Ismail, Yasar Luqman Ahmed, Gert Bange, Dieter Kressler, Otto Berninghausen, Irmi Sinning, Ed Hurt and Roland Beckmann (2017)
Architecture of a ribosome assembly bound to a combined remodeling-checkpoint machinery. Nature Struct. Mol. Biol. 23, 37-44 doi:10.1038/nsmb.3132.
Clara Barrio-Garcia, Matthias Thoms, Dirk Flemming, Lukas Kater, Jochen Bassler, Roland Beckmann and Ed Hurt (2016)
Architecture of the 90S pre-ribosome: a structural view on the birth of the eukaryotic ribosome. Cell 166, 380–393. Markus Kornprobst, Martin Turk, Nikola Kellner, Jing-Dong Cheng, Dirk Flemming, Martin Koš, Isabelle Ko&scaron, Matthias Thoms, Otto Berninghausen, Roland Beckmann and Ed Hurt (2016)
Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome. Cell, 166(2), 380–393. https://doi.org/10.1016/j.cell.2016.06.014 Kornprobst, M., Turk, M., Kellner, N., Cheng, J., Flemming, D., Koš-Braun, I., Koš, M., Thoms, M., Berninghausen, O., Beckmann, R., & Hurt, E. (2016)
Concerted removal of the Erb1-Ytm1 complex in ribosome biogenesis relies on an elaborate interface, Nucl. Acids Res. 44: 926-39. Thoms, M., Ahmed. Y.L., Maddi, K., Hurt, E. & Sinning, I. (2016)
Coupling ribosome stalk biogenesis with nuclear export factor recruitment to the nascent 60S subunit. Nat. Struct. Mol. Biol. 23, 1074-1082.
Anshuk Sarkar, Markus Pech, Matthias Thoms, Dirk Flemming, Roland Beckmann and Ed Hurt (2016)
Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies. Sci Rep. 5:15401. doi: 10.1038/srep15401.
Nikola Kellner, Johannes Schwarz, Miriam Sturm, Javier Fernandez-Martinez, Sabine Griesel, Wenzhu Zhang, Brian Chait, Michael P. Rout, Ulrich Kück and Ed Hurt (2016)
Molecular architecture of the inner ring scaffold of the human nuclear pore complex. Science 352, 363-365. doi: 10.1126/science.aaf0643.
Jan Kosinski, Shyamal Mosalaganti, Alexander von Appen, Roman Teimer, Amanda DiGuilio, William Wan, Huy Khahn Bui, Wim J.H. Hagen, John A.G. Briggs, Joseph S. Glavy, Ed Hurt and Martin Beck (2016)
Role of cation-dependent Nug1 GTPase in recruiting Dbp10 helicase to the undeveloped peptidyl transferase center of the pre-ribosome. Nucl Acid Res. doi: 10.1093/nar/gkw045.
George Manikas, Emma Thomson and Ed Hurt (2016)
A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation, J. Cell Biol. 210: 169-70. Bassler, J., Paternoga, H., Holdermann, I., Thoms, M., Grannemann, S., Barrio-Garcia, C., Nyarko, A., Lee, W. Stier, G., Clark, S., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning*, I. & Hurt*, E. (2015)
Architecture of a ribosome assembly bound to a combined remodeling-checkpoint machinery. Nature Struct. Mol. Biol.
Clara Barrio-Garcia, Matthias Thoms, Dirk Flemming, Lukas Kater, Jochen Bassler, Roland Beckmann and Ed Hurt (2015)
Architecture of the nuclear pore inner ring complex. Science 350, 56-64.
Tobias Stuwe, Christopher J. Bley, Karsten Thierbach, Stefan Petrovic, Sandra Schilbach, Daniel J. Mayo, Thibaud Perriches, Emily J. Rundlet, Young Jeon, Leslie N. Collins, Daniel H. Lin, Marcin Paduch, Akiko Koide, Vincent Lu, Jessica Fischer, Ed Hurt, Shohei Koide, Anthony A. Kossiakoff, André Hoelz (2015)
Characterization of the Chaetomium thermophilum TREX-2 complex and its interaction with the mRNA nuclear export factor MEX67:MTR2. Structure 23, 1246-1257.
Lyudmila Dimitrova, Eugine Valkow, Shinataro Aibara, Dirk Flemming, Steven McLaughlin, Ed Hurt and Murray Stewart (2015)
Coordinated ITS2 rRNA processing by the Las1 complex integrating endonuclease, polynucleotide kinase and exonuclease activities Mol. Cell, 60(5):808-15. doi: 10.1016/j.molcel.2015.10.021.
Lisa Gasse, Dirk Flemming and Ed Hurt (2015)
Coordinated ribosomal L4 protein assembly into the pre-ribosome is regulated by its eukaryote-specific extension. Mol. Cell 58, 854-862.
Philipp Stelter, Ferdinand Huber, Ruth Kunze, Dirk Flemming, André Hoelz and Ed Hurt (2015)
Direct and high throughput (HT) interactions on the ribosomal surface by iRIA. Sci Rep. 5:15401. doi: 10.1038/srep15401. Elisa Pesce, Claudia Minici, Jochen Bassler, Ed Hurt, Massimo Degano, Piera Calamita and Stefano Biffo (2015)
Einblicke in die 'Kinderstube' während der Ribosomen-Entwicklung. Biospektrum (Springer Verlag) 02.15, 144-147.
Jochen Baßler und Ed Hurt (2015)
Linker Nups connect the nuclear pore complex inner ring with outer ring and transport channel. Nature Struct. Mol. Biol. 22, 774-81.
Jessica Fischer, Roman Teimer, Stefan Amlacher, Ruth Kunze and Ed Hurt (2015)
NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export. Nucl. Acid Res 3, 1894-904.
Jun Katahira, Lyudmila Dimitrova, Yumiko Imai, Ed Hurt (2015)
Structural basis for assembly and function Nup82 complex in the nuclear pore scaffold. J. Cell Biol. 208, 283-297 doi/10.1083/jcb.201411003.
Monika Gaik, Dirk Flemming, Alexander von Appen, Panagiotis Kastritis, Philipp Stelter, Khanh Huy Bui, Alessandro Ori, Jochen Bassler, Jessica Fischer, Elisar Barbar, Martin Beck and Ed Hurt (2015)
Structural characterization of the principal mRNA export factor Mex67:Mtr2 from Chaetomium thermophilum. Acta Cryst.F71, 876-888.
Shintaro Aibara, Eugene Valkov, Meindert Lamers, Lyudimila Dimitrova, Ed Hurt and Murray Stewart (2015)
Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site. Nat Commun 6, 6510. https://doi.org/10.1038/ncomms7510 Calviño, F.R., Kharde, S., Ori, A., Hendricks, A., Wild, K., Kressler, D., Bange, G., Hurt, E., Beck, M. & Sinning, I. (2015)
The exosome is recruited to RNA substrates through specific adapter proteins. Cell 162, 1029-1038.
Matthias Thoms, Emma Thomson Jochen Baßler, Marén Gnädig, Sabine Griesel and Ed Hurt (2015)
Toward understanding nuclear pore complex architecture and dynamics in the age of integrative structural analysis. Curr. Opin. Cell Biol. 34:31-38. doi: 10.1016/j.ceb.2015.04.009.
Ed Hurt und Martin Beck (2015)
60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle, Nat. Commun. 5: 3491. doi: 10.1038/ncomms4491. Leidig, C., Thoms, M., Holdermann, I., Bradatsch, B., Berninghausen, O., Bange, G., Sinning, I., Hurt, E. & Beckmann, R. (2014)
A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation, J. Cell Biol. 207: 481-498. Bassler, J., Paternoga, H., Holdermann, I., Thoms, M., Grannemann, S., Barrio-Garcia, C., Nyarko, A., Stier, G., Clark, S., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning*, I. & Hurt*, E. (2014)
A pulse-chase epitope labeling to study cellular dynamics of newly synthesized proteins: a novel strategy to characterize NPC biogenesis and ribosome maturation/export,. Methods in Cell Biol. 122C,147-163. doi: 10.1016/B978-0-12-417160-2.00007-2 .
Philipp Stelter and Ed Hurt (2014)
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum. Nucleic acids research, 42(22), 13525–13533. https://doi.org/10.1093/nar/gku1147 Bock, T., Chen, W. H., Ori, A., Malik, N., Silva-Martin, N., Huerta-Cepas, J., Powell, S. T., Kastritis, P. L., Smyshlyaev, G., Vonkova, I., Kirkpatrick, J., Doerks, T., Nesme, L., Baßler, J., Kos, M., Hurt, E., Carlomagno, T., Gavin, A. C., Barabas, O., Müller, C. W., van Noort, V., Beck, M., & Bork, P. (2014)
Catalytic Rio1 kinase/ATPase mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes. Nucl. Acid Res. 1, doi: 10.1093/nar/gku542 .
Sébastien Ferreira-Cerca, Irene Kiburu, Emma Thomson, Nicole Laronde- Leblanc and Ed Hurt (2014)
Coupled GTPase and remodeling ATPase activities form a checkpoint for ribosome export. Nature 505, 112-116 .
Yoshitaka Matsuo, Sander Granneman, Matthias Thoms, Rizos-Georgios Manikas, David Tollervey and Ed Hurt (2014)
Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192 and karyopherins. Proc. Natl. Acad. Sci. USA 18, 2530-2535 .
Tobias Stuwe, Daniel H. Lin, Leslie Collins, Ed Hurt and André Hoelz (2014)
Functional reconstitution of de novo mitochondrial Fe/S cluster synthesis reveals an essential role of ferredoxin. Nature Com. 5, doi:10.1038/ncomms6013 .
Stefan Amlacher, Ed Hurt, Ulrich Mühlenhoff, Lucia Banci and Roland Lill (2014)
Functional reconstitution of mitochondrial Fe/S cluster synthesis on Isu1 reveals the involvement of ferredoxin. Nat Commun 5, 5013. https://doi.org/10.1038/ncomms6013 Webert, H., Freibert, S.-A., Gallo, A., Heidenreich, T., Linne, U., Amlacher, S., Hurt, E., Mühlenhoff, U., Banci, L., & Lill, R. (2014)
Utilizing the Dyn2-dimerization- zipper as a tool to probe NPC structure and function. Methods in Cell Biol. 122C, 99-115. doi: 10.1016/B978-0-12-417160-2.00005-9 .
Dirk Flemming, Philipp Stelter and Ed Hurt (2014)
Consistent mutational paths predict eukaryotic thermostability. BMC evolutionary biology, 13, 7. https://doi.org/10.1186/1471-2148-13-7 van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S., Bange, G., Creevey, C., Falk, S., Mende, D. R., Sinning, I., Hurt, E., & Bork, P. (2013)
Eukaryotic Ribosome Biogenesis at a glance. J. Cell Sci. in press. https://doi.org/10.1242/jcs.111948 Emma Thomson, Sébastien Ferreira-Cerca and Ed Hurt (2013)
New twist to nuclear import: When two travel together, Com. & Int. Biol. 6:4, e24792; July/August 2013, Landes Bioscience). Bange, G. , Murat, G., Sinning, I., Hurt, E. & Kressler, D. (2013)
Protein interfaces of the Conserved Nup84 Complex from Chaetomium thermophilum revealed by cross-linking mass spectrometry and electron microscopy. Structure 21, 9, 1672-1682.
Karsten Thierbach, Alexander von Appen, Matthias Thoms, Martin Beck, Dirk Flemming and Ed Hurt (2013)
Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae. Nucl. Acid Res. 41, 1191-1200.
Thomas Hierlmeier, Juliane Merl, Martina Sauert, Jorge Perez-Fernandez, Patrick Schultz, Astrid Bruckmann, Stephan Hamperl, Uli Ohmayer, Joachim Griesenbeck, Reinhard Rachel, Anja Jakob, Kristin Hergert, Rainer Deutzmann, Ed Hurt, Philipp Milkereit, Jochen Baßler and Herbert Tschochner (2013)
Structural basis of histone H2A-H2B recognition by the essential chaperone FACT. Nature 499, 111-114.
Maria Hondele, Tobias Stuwe, Markus Hassler, Felix Halbach, Bianca Nijmeijer, Vladimir Rybin, Stefan Amlacher, Ed Hurt and Andreas Ladurner (2013)
Structural characterization of a eukaryotic co-translational chaperone, the ribosome-associated complex (RAC), Nat. Struct. Mol. Biol. 20:23-8. Leidig, C., Bange, G., Kopp, J., Amlacher, S., Aravind, A., Wickles, S., Witte, G., Hurt, E., Beckmann, R. & Sinning, I. (2013)
Structures of the nuclear export receptor CRM1 in its free state reveal major structural rearrangements upon cargo binding. Proc. Natl. Acad. Sci. USA 110, 960-965 .
Thomas Monecke, David Haselbach, Piotr Neuman, Emma Thomson, Ed Hurt, Holger Stark, Achim Dickmanns and Ralf Ficner (2013)
Analysis of the yeast nucleoporin Nup188 reveals a conserved S-like structure with similarity to karyopherins. J. Struct. Biol. 177, 99-105.
Dirk Flemming, Damien Devos, Johannes Schwarz, Stefan Amlacher, Malik Lutzmann and Ed Hurt (2012)
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit. Nat. Struct. Mol. Biol. 19, 1316-1323.
Sébastien Ferreira-Cerca, Vatsala Sagar, Thorsten Schäfer, Anne-Maria Wesseling, Eileen Chai, Ed Hurt and Nicole LaRonde-LeBlanc (2012)
Functional and structural characterisation of the mammalian TREX-2 complex that links transcription with nuclear mRNA export. Nucl. Acid Res. 40, 4562-4573.
Divyang Jani, Sheila Lutz, Ed Hurt, Ronald Laskey, Murray Stewart and Vihandha Wickramasinghe (2012)
Monitoring Spatiotemporal Biogenesis of Macromolecular Assemblies by Pulse-chase Epitope Labeling. Mol. Cell 47, 788-796.
Matthias Thoms and Ed Hurt (2012)
Monitoring spatiotemporal biogenesis of macromolecular assemblies by pulse-chase epitope labeling. Molecular cell, 47(5), 788–796. https://doi.org/10.1016/j.molcel.2012.06.015 Stelter, P., Kunze, R., Radwan, M., Thomson, E., Thierbach, K., Thoms, M., & Hurt, E. (2012)
Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nat. Struct. Mol. Biol. 19, 328-336.
Andrew M. Ellisdon, Lyudmila Dimitrova, Ed Hurt and Murray Stewart (2012)
Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel. Nat. Struct. Mol. Biol 19, 1234-1241.
Bettina Bradatsch, Christoph Leidig, Sander Granneman, Marén Gnädig, Bettina Böttcher, David Tollervey, Roland Beckmann and Ed Hurt (2012)
Synchronizing nuclear import of ribosomal proteins with ribosome assembly, Science 338: 666-71. Kressler*, D., Bange, G. Ogawa, Y., Bradatsch, B., Pratte, D., Strauß, D., Amlacher, S., Yoneda,Y., Katahira, J., Sinning, I.*, & Hurt, E.* (2012)
The conserved Bud20 zinc finger protein is a new component of the ribosomal 60S subunit export machinery. Mol. Cell Biol., 32, 4898-912.
Jochen Baßler, Isabella Klein, Claudia Schmidt, Emma Thomson, Martina Kallas, Maria Anna Wagner, Gerald Rechberger, Heimo Strohmaier, Ed Hurt,Helmut Bergler (2012)
The power of AAA- ATPases on the road of pre-60S ribosome maturation - molecular machines that strip pre-ribosomal particles. Biochim. Biophys. Acta1823, 92-100.
Dieter Kressler, Ed Hurt, Helmut Bergler and Jochen Baßler (2012)
Bange, Nico Kümmerer, Przemyslaw Grudnik, Robert Lindner, Georg Petzold, Dieter Kressler, Ed Hurt, Klemens Wild and Irmgard Sinning (2012) Structural basis for the molecular evolution of SRP-GTPase activation by protein. Nature Struct. Mol. Biol. 18, 1376-1380.
Dieter Kressler, Ed Hurt, Helmut Bergler and Jochen Baßler (2011)
Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile. Cell 146, 277-289, July 22.
Stefan Amlacher, Phillip Sarges, Dirk Flemming, Vera van Noort, Ruth Kunze, Damien Devos, Manimozhiyan Arumugam, Peer Bork and Ed Hurt (2011)
Linking gene regulation to mRNA production and export. Curr. Opinion Cell Biol. 23, 302-309.
Susana Rodriguez-Navarro and Ed Hurt (2011)
Probing the FG repeat network of nucleoporins defines structural and functional features of the nuclear pore complex. J. Cell Biol. 195, 183-192.
Philipp Stelter, Ruth Kunze, Jessica Fischer and Ed Hurt (2011)
Structural basis for the molecular evolution of SRP-GTPase activation by protein. Nat Struct Mol Biol. 8:1376-80. Bange G, Kümmerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I. (2011)
Driving Ribosome Assembly. Biochim. Biophys. Acta 1803, 673-683.
Dieter Kressler, Ed Hurt and Jochen Bassler (2010)
Gene regulation by nucleoporins and links to cancer. Mol. Cell 38, 6-15.
Alwin Köhler and Ed Hurt (2010)
Precise mapping of subunits in multiprotein complexes by a versatile EM-label. Nature Struct. Mol. Biol. in press.
Dirk Flemming, Karsten Thierbach, Philipp Stelter, Bettina Boettcher and Ed Hurt (2010)
Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module. Cell 141, 606-617.
Alwin Köhler, Eric Zimmermann, Maren Schneider, Ed Hurt and Ning Zheng (2010)
Structural basis for the interaction between yeast Spt-Ada-Gcn5 acetyltransferase (SAGA) complex components Sgf11 and Sus1. J. Biol. Chem. 285, 3850-3856.
Andrew M. Ellisdon, Divyang Jani, Alwin Köhler, Ed Hurt and Murray Stewart (2010)
The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly. Mol. Cell in press.
Jochen Baßler, Martina Kallas, Matthias Thoms, Cornelia Ulbrich, Brigitte Pertschy and Ed Hurt (2010)
The structure of Get4 reveals an alpha-solenoid fold adapted for multiple interactions in tail-anchored protein biogenesis. FEBS Lett. 584(8):1509-14. Bozkurt G, Wild K, Amlacher S, Hurt E, Dobberstein B, Sinning I. (2010)
A toxic nucleoporin mutant induces abarrent nucleoporin foci in the cytoplasm. J. Cell Biol. 185, 387-396.
Dirk Flemming, Philipp Sarges, Philipp Stelter, Andrea Hellwig, Bettina Boettcher and Ed Hurt (2009)
An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes. PLoS Biology 7 e8.
Michal Skruzny, Claudia Schneider, Attila Racz, Julan Weng, David Tollervey and Ed Hurt (2009)
Arrest by Ribosome. Nature 459, 46-47.
Sébastien Ferreira-Cerca and Ed Hurt (2009)
Linear ubiquitin fusion to Rps31 and its subsequent cleavage 1 are required for the efficient production and functional integrity of 40S ribosomal subunits. Mol. Microbiol. 72, 69-84.
Thierry Lacombe, Juan J. García-Gómez, Jesús de la Cruz, Daniela Roser, Ed Hurt, Patrick Linder and Dieter Kressler (2009)
Mechanochemical Removal of Ribosome Biogenesis Factors from Nascent 60S Ribosomal Subunits. Cell 138, 911-922.
Cornelia Ulbrich, Meikel Diepholz, Jochen Baßler, Dieter Kressler, Brigitte Pertschy, Kiki Galani, Bettina Böttcher and Ed Hurt (2009)
Mutational Uncoupling of Sus1's role in NPC-targeting of an mRNA Export Complex and Histone H2B deubiquitination J. Biol. Chem. 284, 12049-12056.
Christoph Klöckner, Maren Schneider, Sheila Lutz, Dieter Kressler, Divyang Jani, Murray Stewart, Ed Hurt and Alwin Köhler (2009)
Nucleus-Specific and Cell Cycle-Regulated Degradation of MAPK Scaffold Protein Ste5 Contributes to the Control of Signaling Competence. Mol. Cell Biol. 29, 582-601.
Lindsay S. Garrenton, Andreas Braunwarth, Stefan Irniger, Ed Hurt, Markus Künzler and Jeremy Thorner (2009)
Purification of poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein (mRNP) core structure. J.Biol.Chem. 284, 354911-354917.
Julien Batisse, Claire Batisse, Aidan Budd, Bettina Böttcher and Ed Hurt (2009)
RNA helicase Prp43 and its co-factor Pfa1 promote 20S to 18S rRNA processing catalyzed by the endonuclease Nob1. J.Biol.Chem. 284, 35079-35091.
Brigitte Pertschy, Claudia Schneider, Maren Gnädig, Thorsten Schäfer, David Tollervey and Ed Hurt (2009)
Sem1 is a functional component of the nuclear pore complex associated mRNA export machinery. J. Cell Biol. 184, 833-846.
Marius Boulos Faza, Stefan Kemmler, Sonia Jimeno, Christina González-Aguilera, Andres Aguilera, Ed Hurt and Vikram Govind Panse (2009)
Structural basis for the Sus1:Sgf11 interaction in the yeast SAGA transcription activation complex. J. Biol. Chem. in press.
Andrew M. Ellisdon, Divyang Jani, Alwin Köhler, Ed Hurt and Murray Stewart (2009)
Structural insights into tail-anchored protein binding and membrane insertion by Get3. Proc Natl Acad Sci U S A. 106(50):21131-6. Bozkurt G, Stjepanovic G, Vilardi F, Amlacher S, Wild K, Bange G, Favaloro V, Rippe K, Hurt E, Dobberstein B, Sinning I. (2009)
Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export. Mol Cell 33, 727-737. Jani, D., Lutz, S., Marshall, N.J., Fischer, T., Kohler, A., Ellisdon, A.M., Hurt, E., and Stewart, M. (2009)
The adaptor Aly and co-adaptor Thoc5 function in Tap-p15 mediated nuclear export of HSP70 mRNA. EMBO J. 28, 556-567.
Jun Katahira, Hitomi Inoue, Ed Hurt, Yoshihiro Yoneda (2009)
A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export. EMBO J. 27, 6–16.
Wei Yao, Malik Lutzmann and Ed Hurt (2008)
Der Kernporenkomplex oder das Tor zur Welt des Zytoplasmas. Im Jahrbuch 2007 der Deutschen Akademie der Naturforscher Leopoldina. Leopoldina 53, 457-460.
Ed Hurt (2008)
Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc Natl Acad Sci U S A 105, 11731-11736. → Highlighted by the Faculty of 1000 (F1000.com/1119165). http://www.pnas.org/content/105/33/11731 Beck, R, Sun, Z, Adolf, F, Rutz, C, Bassler, J, Wild, K, Sinning, I, Hurt, E, Brügger, B, Béthune, J*, and Wieland, F* (2008)
Structural basis of the Nic96 subcomplex organization in the nuclear pore channel. Mol. Cell 29, 46-55.
Nils Schrader, Philipp Stelter, Dirk Flemming, Ruth Kunze, Ed Hurt* and Ingrid Vetter* (*corresponding authors) (2008)
The AAA-ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles. J. Cell Biol. 181, 835-844.
Dieter Kressler, Daniela Roser, Brigitte Pertschy and Ed Hurt (2008)
The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression. J Cell Biol 182, 897-910. Grund, S.E., Fischer, T., Cabal, G.G., Antunez, O., Perez-Ortin, J.E., and Hurt, E. (2008)
Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export. Nat. Cell Biol. 10, 707-715.
Alwin Köhler, Maren Schneider, Ghislain Cabal, Ulf Nehrbass and Ed Hurt (2008)
Adjunct duties for karyopherins: Regulating septin sumoylation. Dev. Cell 12, 669-670.
Vikram Govind Panse and Ed Hurt (2007)
Arx1 functions as alternative nuclear export receptor for the 60S pre-ribosomal subunit. Mol. Cell 27, 767-779.
Bettina Bradatsch, Jun Katahira, Eva Kowalinski, Gert Bange, Wei Yao, Toshihiro Sekimoto, Viola Baumgärtel, Guido Boese, Jochen Bassler, Klemens Wild, Reiner Peters, Yoshihiro Yoneda, Irmi Sinning and Ed Hurt (2007)
Exporting RNA from the nucleus to the cytoplasm. Nature Rev. Mol. Cell Biol. 8, 761-773.
Alwin Köhler and Ed Hurt (2007)
Molecular basis for the functional interaction of dynein light chain with the nuclear pore complex. Nature Cell Biology 9, Number 7, 788-796.
Philipp Stelter, Ruth Kunze, Dirk Flemming, Dominic Höpfner, Meikel Diepholz, Peter Philipsen, Bettina Böttcher and Ed Hurt (2007)
Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2. Mol. Cell 26, 51-62.
Wei Yao, Daniela Roser, Alwin Köhler, Bettina Bradatsch, Jochen Baßler and Ed Hurt (2007)
The crystal structure of Ebp1 reveals how the methionine aminopeptidase fold serves as a binding platform for multiple interactions. FEBS Lett. 581, 4450-4454.
Eva Kowalinski, Gert Bange, Bettina Bradatsch, Ed Hurt, Klemens Wild and Irmi Sinning (2007)
A round of phosphorylation/de-phosphorylation triggers maturation of the pre-ribosomal 40S subunit. Nature 441, 651-655.
Thorsten Schäfer, Bohumil Maco, Elisabeth Petfalski, David Tollervey, Bettina Boettcher, Ueli Aebi and Ed Hurt (2006)
Coordinated nuclear import of RNA polymerase III subunits. Traffic 7, 465-473.
Ulrike Hardeland and Ed Hurt (2006)
Formation and nuclear export of pre-ribosomes are functionally linked to the small-ubiquitin-related (SUMO) modifier pathway. Traffic 7, 1311-1321.
Vikram Govind Panse, Dieter Kressler, Andrea Pauli, Elisabeth Petfalski, Marén Gnädig, David Tollervey and Ed Hurt (2006)
Molecular Analysis of SAGA Mediated Gene Gating In Yeast. Nature 441, 770-773.
Ghislain Cabal, Susana Rodrigez-Navarro, August Genevesio, Jean-Christophe Olivo-Marin, Christophe Zimmer, Olivier Gadal, Frank Feuerbach-Fournier, Annick Lesne, Henri Buc, Ed Hurt* and Ulf Nehrbass* (*corresponding authors) (2006)
The mRNA Export Factor Sus1 Is Involved in SAGA-mediated H2B Deubiquitinylation through Its Interaction with Ubp8 and Sgf11. Mol. Biol. Cell. 17, 4228-4236.
Alwin Köhler, Pau Pascual-García, Ana Llopis, Meritxell Zapater, Francesc Posas, Ed Hurt and Susana Rodríguez-Navarro (2006)
The Nug1 GTPase reveals an N-terminal RNA binding domain that is essential for association with pre-60S subunits. J. Biol. Chem. 281, 24737-24744.
Jochen Baßler, Martina Kallas and Ed Hurt (2006)
TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients. EMBO J. 25, 3832-3842.
Yoshimi Honma, Aiko Kitamura, Ryo Shioda, Hironori Maruyama, Kanako Ozaki, Yoko Oda, Thierry Mini, Paul Jen, Yasushi Maki, Kazuhito Yonezawa, Ed Hurt, Masaru Ueno, Masahiro Uritani, Michael N. Hall, and Takashi Ushimaru (2006)
Functional link between ribosome formation and biogenesis of iron-sulfur proteins. EMBO J. 24, 580-588.
Alexander Yarunin, Vikram Panse, Elisabeth Petfalski, Christophe Dez, David Tollervey and Ed Hurt (2005)
Reconstitution of Nup157 and Nup145N into the Nup84 complex. J. Biol. Chem. 280, 18442-18451.
Malik Lutzmann, Ruth Kunze, Karin Stangl, Philipp Stelter, Katalin Fejes Tóth, Bettina Boettcher and Ed Hurt (2005)
Recruitment of the human TREX complex to mRNA during splicing Genes and Develop.19, 1512-1517.
Seiji Masuda, Rita Das, Ed Hurt, Nijsje Dorman and Robin Reed (2005)
The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus by an Xpo1p-dependent mechanism. FEBS Lett. 579, 969-975.
Kyriaki Galani, Eduard C. Hurt and George Simos (2005)
A nascent pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol. Cell 15, 295-301.
Tracy A. Nissan, Kyriaki Galani, Bohumil Maco, David Tollervey, Ueli Aebi and Ed Hurt (2004)
A proteome-wide approach identifies sumolyated substrate proteins in yeast. J. Biol. Chem., in press.
Vikram Govind Panse, Ulrike Hardeland, Thilo Werner, Bernhard Kuster and Ed Hurt (2004)
Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. PNAS Vol. 101, no. 7, 1858-1862.
Ed Hurt, Ming-juan Luo, Susanne Röther, Robin Reed and Katja Sträßer (2004)
Rea1, a dynein-related nuclear AAA-type ATPase, is involved in late 25S rRNA processing and nuclear export of 60S subunits. J. Biol. Chem. 279, 55411-55418.
Kyriaki Galani, Tracy A. Nissan, Elisabeth Petfalski, David Tollervey and Ed Hurt (2004)
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Cell. 116(1):75-86. Rodriguez-Navarro S, Fischer T, Luo MJ, Antunez O, Brettschneider S, Lechner J, Perez-Ortin JE, Reed R, Hurt E. (2004)
The Yeast Kinase Swe1 is Required for proper Entry into a new Cell Cycle after Arrest due to Ribosome Biogenesis Defects. Cell Cycle 3, 648-54.
Francesca Saracino, Jochen Baßler, Diego Muzzini, Ed Hurt and Maria Luisa Agostoni Carbone (2004)
Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat. Cell Biol. 6, 840—848. Tamás Fischer, Susana Rodriguez-Navarro, Elmar Schiebel and Ed Hurt (2004)
A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits. The Journal of Biological Chemistry, Vol. 278, No. 6, pp. 4072-4081. Milkereit, P., Strauss, D., Bassler, J., Gadal, O., Kühn, H., Schütz, S., Gas, N., Lechner, J., Hurt, E., & Tschochner, H. (2003)
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 4(12):1156-62. Neumann S, Petfalski E, Brügger B, Grosshans H, Wieland F, Tollervey D, Hurt E. (2003)
Identification and characterization of a novel RanGTP binding protein Yrb30. J. Biol. Chem. 278, 15397-15405. Andreas Braunwarth, Michel Fromont-Racine, Pierre Legrain, Ralf Bischoff, Thomas Gerstberger, Ed Hurt and Markus Künzler (2003)
Pre-Ribosomes on the Road from the Nucleolus to the Cytoplasm. Trends Cell Biol. 13, 255-263. Herbert Tschochner and Ed Hurt (2003)
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes. EMBO J. 22, 1370-1380.
Thorsten Schäfer, Daniela Strauß, Elisabeth Petfalski, David Tollervey and Ed Hurt (2003)
Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins. Nature Cell Biology, Vol. 5, 21-27.
Panse V., Küster B., Gerstberger Th., Hurt E. (2003)
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm. EMBO J. 2002 Oct 15; 21(20):5539-47.
Nissan T., Bassler J., Petfalski E., Tollervey D., Hurt E. (2002)
90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell. 2002 Jul; 10(1):105-15.
Grandi P., Rybin V., Bassler J., Petfalski E., Strauss D., Marzioch M., Schaefer T., Kuster B., Tschochner H., Tollervey D., Gavin AC., Hurt E. (2002)
A conserved mRNA export machinery coupled to pre-mRNA splicing. Cell 108, 523-531.
Robin Reed and Ed Hurt (2002)
An intron in the YRA1 gene is required to control Yra1p protein expression and mRNA export. EMBO Rep. 3, 438-442.
Susana Rodríguez-Navarro, Katja Sträßer and Ed Hurt (2002)
Complex Formation between Tap and p15 Affects Binding to FG-repeat Nucleoporins and Nucleocytoplasmic Shuttling. J. Biol. Chem., 11, No. 11, March, 9242-9246.
Jun Katahira, Katja Straesser, Takuya Saiwaki, Yoshihiro Yoneda, and Ed Hurt (2002)
Modular Self-Assembly of a Y-Shaped Multiprotein Complex from Seven Nucleoporins. EMBO J., 21, No. 3, 387-397.
Malik Lutzmann, Ruth Kunze, Andrea Buerer, Ueli Aebi and Ed Hurt (2002)
Nuclear export of tRNA. Results Probl. Cell Differ. 35, 115-31.
Simos G, Grosshans H, Hurt E. (2002)
Rlp7p is associated with 60S pre-ribosomes, restricted to the granular component of the nucleolus and required for pre-rRNA processing. J. Cell Biol. in press.
Olivier Gadal, Daniela Strauß, Elisabeth Petfalski, Pierre-Emmanuel Gleizes, Nicole Gas, David Tollervey, and Ed Hurt (2002)
Structure of the C-terminal FG-Nucleoporin binding domain of Tap/NXF1. Nat. Struct. Biol. 9, 247-251. (reprints not available)
Richard Grant , Ed Hurt, David Neuhaus and Murray Stewart (2002)
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. Embo J. 21(21): 5843-5852. https://doi.org/10.1093/emboj/cdf590 Fischer, T., Sträßer, K., Rácz, A., Rodriguez-Navarro, S., Oppizzi, M., Ihrig, P., Lechner, J., & Hurt, E. (2002)
TREX is a conserved complex coupling transcription with mRNA export. Nature 417, 304-308.
Katja Sträßer, Seiji Masuda, Paul Mason, Jens Pfannstiel, Marisa Oppizzi, Susana Rodriguez-Navarro, Ana G. Rondón, Andres Aguilera, Kevin Struhl, Robin Reed and Ed Hurt (2002)
A nuclear AAA-type ATPase (Rix7p) is required for large ribosomal subunit biogenesis and subsequent export to the cytoplasm. EMBO J., 20, 3695-3704.
Olivier Gadal, Daniela Strauß, Joris Braspenning, Dominic Hoepfner, Elisabeth Petfalski, Peter Philippsen, David Tollervey and Ed Hurt (2001)
Arc1p Organizes the Yeast Aminoacyl-tRNA Synthetase Complex and Stabilizes Its Interaction with the Cognate tRNAs. J. Biol. Chem. 276, No. 8, February, 6000-6008.
Karina Deinert, Franco Fasiolo, Eduard C. Hurt and George Simos (2001)
Biogenesis of the Signal Recognition Particle Requires Nuclear Import of SRP proteins, Assembly with the SRP-RNA in the Nucleolus, and Xpo1p-mediated Nuclear Export. J. Cell Biol. 153, 745-62.
Helge Großhans, Karina Deinert, Ed Hurt and George Simos (2001)
Elongation factor like-1, a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2 is required for ribosome export and a late cytoplasmic step of ribosomal assembly. Molecular Cell, 8, 1363-1373.
B. Senger, D. Lafontaine, O. Gadal, J. Graindorge, A. Camasses, A.Sanni, J. Garnier, M. Breitenbach, E. Hurt, and F. Fasiolo (2001)
Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell 8:517-29. Bassler, J., Grandi, P., Gadal, O., Lessmann, T., Petfalski, E., Tollervey, D., Lechner, J., & Hurt, E. (2001)
Maturation of Pre-Ribosomes Requires Noc-Proteins and is Coupled to Transport from the Nucleolus to the Nucleoplasm. Cell 105, 499-509. Philipp Milkereit, Olivier Gadal, Alexander Podtelejniko V., Stephanie Trumtel, Nicole Gas, Elisabeth Petfalski, David Tollervey, Mathias Mann, Ed Hurt and Herbert Tschochner (2001)
Mutations in the YRB1 Gene Encoding Yeast Ran-Binding-Protein-1 That Impair Nucleocytoplasmic Transport and Suppress Yeast Mating Defects. Genetics 157, 1089-1105.
Künzler M., Trueheart J., Sette C., Hurt E., Thorner J. (2001)
Nmd3p functions as a nuclear adaptor for Xpo1p-dependent export of 60S ribosomal subunits. Mol. Cell. Biol. 21, 3405-3415.
Olivier Gadal, Daniela Strauss, Jacques Kessl, Bernard Trumpower, David Tollervey and Ed Hurt (2001)
Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is compromised in yeast. J. Biol. Chem. 276, 46333-46339.
Helge Großhans, Francois Lecointe, Henri Grosjean Ed Hurt, and George Simos (2001)
Regulation of intracellular location of aminoacyl-tRNA-synthetases by complex formation with Arc1p. EMBO J. 20, 6889-6898.
K. Galani, H. Großhans, Deinert, K., E. C. Hurt,and G. Simos (2001)
Targeting of Ran: variation on a common theme? J. Cell Sci. 114, 3233-3241.
Markus Künzler and Ed Hurt (2001)
The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Mol. Cell. Biol., 21, 7944-7955.
Susanne M. Bailer, Carolin Balduf, and Ed Hurt (2001)
The splicing factor Sub2p interacts directly with the mRNA transport factor Yra1p and is required for nuclear mRNA export. Nature 413, 648-652.
Katja Sträßer and Ed Hurt (2001)
A Generic Strategy to Analyze the Spatial Organization of Multi-Protein Complexes by Cross-Linking and Mass Spectrometry. Anal Chem.72, 267-75.
Juri Rappsilber, Symeon Siniossoglou, Eduard C. Hurt and Matthias Mann (2000)
An aminoacylation-dependent nuclear tRNA export pathway in yeast. Genes &. Dev. 14, 830-840.
Grosshans, H., E.C. Hurt and G. Simos (2000)
Binding of the Mex67p/Mtr2p Heterodimer to FXFG-, GLFG-, and FG-Repeat Nucleoporins is essential for Nuclear mRNA Export. J. Cell Biol., 150, 695-706.
Katja Sträßer, Jochen Bassler and Ed Hurt (2000)
Cloning and characterization of the Schizosaccharomyces pombe tRNA: pseudouridine synthase Pus1p. Nucleic Acids Research, Vol. 28, No. 23, 4604-4610.
Klaus Hellmuth, Henri Grosjean, Yuri Motorin, Karina Deinert, Ed Hurt and George Simos (2000)
Comparative spatial localization of Protein-A-tagged and authentic yeast nuclear pore complex proteins by immunogold electron microscopy. J. Struct. Biol.129, 295-305.
Birthe Fahrenkrog, John Aris, Ed Hurt, Nelly Panté and Ueli Aebi (2000)
Mex67p Mediates the Nuclear Export of a Variety of Pol II Transcripts. J. Biol. Chem. 275, 8361-8368.
Ed Hurt, Katja Sträßer, Alexandra Segref, Nick Schlaich, Carlo Presutti, David Tollervey and Ralf Jansen (2000)
Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with Nic96p. J. Biol. Chem. 275:343-350.
Kosova, B., N. Panté, C.Rollenhagen, A. Podtelejnikov, M. Mann, U. Aebi and E. Hurt (2000)
Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J. Biol. Chem. 275, 23540-23548.
Susanne Bailer, C. Balduf, J. Katahira, A. Podtelejnikov, Ch. Rollenhagen, M. Mann, N. Panté and Ed Hurt (2000)
Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast. J. Biol. Chem. 275, 19352-19360.
Symeon Siniossoglou, Ed Hurt and Hugh R. B. Pelham (2000)
Purification of ProteinA-Tagged Yeast Ran Reveals Association with a Novel Karyopherin beta Family Member Pdr6p. J. Biol. Chem. 275, 467-471. Denise Lau, Markus Künzler, Andreas Braunwarth, Klaus Hellmuth, Alexandre Podtelejnikov, Matthias Mann and Ed Hurt (2000)
Structure and Assembly of the Nup84p Complex. J. Cell. Biol. 149, 41-53.
Symeon Siniossoglou, Malik Lutzmann, Helena Santos-Rosa, Kevin Leonard, Shirley Mueller, Ueli Aebi and Ed Hurt (2000)
The protein ALY kinks pre-messenger-RNA splicing to nuclear export in metazoans. Nature, 407, 401-405.
Zhaolan Zhou, Ming-juan Luo, Katja Sträßer, Jun Katahira, Ed Hurt and Robin Reed (2000)
Transport of tRNA Out of the Nucleus - Direct Channeling to the Ribosome? J. Struct. Biol.129, 288-294.
Helge Grosshans, George Simos and Ed Hurt (2000)
Yeast Nuclear Pore Complex Assembly Defects Determined by Nuclear Envelope Reconstruction. J. Struct. Biol., Vol. 132, No. 1, October 2000, 1-5.
Natalia Gomez-Ospina, Garry Morgan, Thomas H. Giddings Jr., Buket Kosova, Ed Hurt and Mark Winey (2000)
Yeast Ran-binding protein 1 (Yrb1) shuttles between nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway. Mol. Cell Biol. 20, 4295-4308.
Markus Künzler, Thomas Gerstberger, Francoise Stutz, and Ed Hurt (2000)
Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export. EMBO J. 19, 410-420.
Katja Sträßer and Ed Hurt (2000)
A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants. J. Cell Biol. 144(3):389-401.
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Nuclear export of mRNA in yeast. FEBS Lett. 452:77 - 81.
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Nup192p is a Conserved Nucleoporin with a Preferential Location at the Inner Site of the Nuclear Membrane. J. Biol. Chem. 274:22646 - 22651.
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Pseudouridine Mapping in the Yeast spliceosomal UsnRNAs Reveals that Pseudouridine Synthase Pus1p Exhibits a Dual Substrate Specificity for U2snRNA and tRNA. Mol. Cell. Biol. 19:2142 - 2154.
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The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J., 18:2593-2609.
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tRNA aminoacylation - a visa to leave the nucleus. Curr. Biol. 9:R238 - R241.
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'Rush Hour' am Zellkern. Ruperto Carola 1, 12 -17.
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A Novel Complex of Membrane Proteins Required for Formation of a Spherical Nucleus. EMBO J. 17, 6449-6464.
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A tRNA-binding domain within Arc1p delivers tRNA to two aminoacyl-tRNA synthetases. Molecular Cell 1, 235 - 242.
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Characterization of yeast protein Deg1 as Pseudouridine synthase (Pus3) catalyzing the formation of Y38 and Y39 in tRNA anticodon loop. J. Biol. Chem. 273, 1316-1323.
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Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett., 433, 185-190.
M. Künzler and E.C. Hurt (1998)
Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes. J. Cell Biol. 143, 577 ? 588.
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Mtr10p Functions as a Nuclear Import Receptor for the mRNA Binding Protein Npl3p. EMBO J. 17, 2196-2207.
B. Senger, G. Simos, F.R. Bischoff, A. Podtelejnikov, M. Mann, and E.C. Hurt (1998)
Nuclear compartments and nuclear machines. Curr. Opin. Cell Biol. 10, 301 - 303.
E. Hurt and G. Schütz (1998)
Nuclear mRNA Export Requires Complex Formation between Mex67p and Mtr2p at the Nuclear Pores. Mol. Cell. Biol. 18(11) 6826-6838.
H. Santos-Rosa, H. Moreno, G. Simos, A. Segref, B. Fahrenkrog, N. Panté, and E.C. Hurt (1998)
Nup116p and Nup100p are Interchangeable through a Conserved Motif which Constitutes a Binding Site for the mRNA Transport Factor Gle2p/Nup40p. EMBO J. 17, 1107-1119.
S. Bailer, S. Siniossoglou, S. Podtelejnikov, A. Hellwig, M. Mann, and E.C. Hurt (1998)
Yeast Los1p Has Properties of an Exportin-Like Nucleocytoplasmic Transport Factor for tRNA. Mol. Cell. Biol. 18(11) 6374-6386.
K. Hellmuth, D. Lau, R. Bischoff, M. Künzler, E.C. Hurt, and G. Simos (1998)
Yeast tRNA: pseudouridine synthase (Pus1p) displays a multisite substrate specificity. RNA 4, 856 - 869.
Y. Motorin, G. Keith, C. Simon, D. Foiret, G. Simos, E. Hurt, and H. Grosjean (1998)
From Nucleoporins to Functional Nuclear Pore Complexes. Curr. Opin. Cell Biol.9, 401 - 411.
V. Doye, and E.C. Hurt (1997)
In Vitro Reconstitution of a Heterotrimeric Nucleoporin Complex Consisting of Recombinant Nsp1p, Nup49p and Nup57p. Mol. Biol. Cell 8,33-46.
N.L. Schlaich, M. Häner, U. Aebi, and E.C. Hurt (1997)
Mex67p, A Novel Factor for Nuclear mRNA Export, Binds to Both Poly(A)+ RNA and Nuclear Pores. EMBO J. 11, 3256 - 3271.
A. Segref, K. Sharma, V. Doye, A. Hellwig, J. Huber, R. Lührmann, and E.C. Hurt (1997)
Nucleolar KKE/D Repeat Proteins Nop56p and Nop58p Interact with Nop1p and Are Required for Ribosome Biogenesis. Mol. Cell. Biol. 17, 7088-7098.
T. Gautier, T. Bergès, D. Tollervey, and E.C. Hurt (1997)
Nup93a, a Vertebrate Homolog of Yeast Nic96p Form a Complex with a Novel 205 kDa Protein and Are Involved in Nuclear Pore Assembly and Nuclear Growth. Mol. Biol. Cell. 8, 2017 - 2038.
P. Grandi, T. Dang, N. Panti, A. Shevchenko, M. Mann, D. Forbes, and E.C. Hurt (1997)
Two Functionally Distinct Domains Generated by in vivo Cleavage of Nup145p: a Novel Biogenesis Pathway for Nucleoporins. EMBO J. 16, 5086 - 5097.
M.T. Teixeira, S. Siniossoglou, S. Podtelejnikov, J.-C. Binichou, M. Mann, E.C. Hurt, and E. Fabre (1997)
Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Ann. Rev. Genet. 31, 277-313.
E. Fabre, and E.C. Hurt (1997)
A novel complex of nucleoporins, which includes Sec13p and Sec13p homolog, is essential for normal nuclear pores. Cell 84, 265 - 275.
S. Siniossoglou, C. Wimmer, M. Rieger, V. Doye, H. Tekotte, C. Weise, S. Emig, A. Segref, and E.C. Hurt (1996)
Analysis of Nic96p Reveals that its Central Domain is Required for Nuclear Pore Formation and Together with its Carboxy-Terminal Domain Functionally Interacts with a Novel Nucleoporin Nup188p. J. Cell Biol. 133, 1141-1152.
U. Zabel, V. Doye, H. Tekotte, R. Wepf, and E.C. Hurt (1996)
Importins/karyopherins meet nucleoporins. Cell 84, 509-515.
E.C. Hurt (1996)
MHP1, an essential gene in Saccharomyces cervisiae required for microtubule function. J. Cell Biology 135, 1323 - 1340.
I. Irminger-Finger, E.C. Hurt, A. Roebuck, M. A. Collart, and S.J. Edelstein (1996)
NBP35 encodes an essential and evolutionary conserved protein in Saccharomyces cerevisiae with homology to a superfamily of bacterial ATPases. Gene 178, 97-106.
G. Vitale, E. Fabre, and E.C. Hurt (1996)
Nuclear Pore Proteins are Required for the Biogenesis of Functional tRNA. EMBO J. 15, 2285-2290.
G. Simos, H. Tekotte, H. Grosjean, A. Segref, K. Sharma, D. Tollervey, and E.C. Hurt (1996)
The Yeast Protein Arc1p binds to tRNA and functions as a Cofactor for the Methionyl- and Glutamyl-tRNA Synthetases. EMBO J. 15, 5437 - 5448.
G. Simos, A. Segref, F. Fasiolo, K. Hellmuth, A. Shevchenko, M. Mann, and E.C. Hurt (1996)
Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol. Cell. Biol. 16, 294-301.
K. Sharma, E. Fabre, H. Tekotte, E.C. Hurt, and D. Tollervey (1996)
A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J. Cell Biol. 130, 1263-1273.
P. Grandi, S. Emig, C. Weise, F. Hucho, T. Pohl, and E.C. Hurt (1995)
Disruption of the gene coding for the nuclear pore protein Nup1p leads to nuclear accumulation of poly(A)+ RNA. Eur. J. Cell Biol. 67, 8 -14.
N.L. Schlaich, and E.C. Hurt (1995)
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J. 14, 76 - 87.
P. Grandi, N.L. Schlaich, H. Tekotte, and E.C. Hurt (1995)
Genetic approaches to nuclear pore structure and function. Trends Genet. 11, 193-199.
V. Doye, and E.C. Hurt (1995)
Nucleocytoplasmic transport - factors and mechanisms. FEBS Lett. 369, 107-112.
G. Simos, and E.C. Hurt (1995)
The nuclear pore complex in yeast. In Membrane Protein Transport. Editor S. S. Rothman, Jai Press Ltd., Vol. 2, 29-56.
P. Grandi, and E.C. Hurt (1995)
A novel nuclear pore protein Nup133p with Distinct Roles in poly(A)+ RNA Transport and Nuclear Pore Distribution. EMBO J. 13, 6062 - 6075.
V. Doye, R. Wepf, and E.C. Hurt (1994)
Nuclear transport. Curr. Opin. Cell Biol. 6, 335-342.
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Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Cell 78, 275 - 289.
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Synthetic lethality with fibrillarin idenitifies NOP77p, a nucleolar protein required for pre-rRNA pprocessing and modification. EMBO J. 13, 3136-3148.
T. Berges, E. Petfalski, D. Tollervey, and E.C. Hurt (1994)
A U3 snoRNP Protein with Homology to Splicing Factor PRP4 and Gb Domains is Required for Ribosomal RNA Processing. EMBO J. 12, 2549-2558.
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Analysis of nucleo-cytoplasmic transport in a thermo-sensitive mutant of nuclear pore protein NSP1. Eur. J. Cell Biol. 62, 1-12.
U. Nehrbass, E. Fabre, S. Dihlmann, W. Herth, and E.C. Hurt (1993)
Approaches towards a genetic analysis of the nuclear pore complex in yeast. In 'Protein Synthesis and Targeting' (eds. A.J.P. Brown, M.F. Tuite and J.E.G. McCarthy). NATO ASI Series, Vol. H 71, pp. 269-281, Springer-Verlag, Berlin-Heidelberg.
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Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J. 12, 3061-3071.
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Reconstitution of nuclear protein transport with semi-intact yeast cells. J. Cell Biol. 123, 785-798.
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Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. Cell 72, 443-457.
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The nuclear pore complex. FEBS Lett. 325, 76-80.
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A New Subclass of Nucleoporins that Functionally Interacts with Nuclear Pore Protein NSP1. EMBO J. 11, 5051-5061.
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Depletion of the nucleoporin NSP1 in yeast inhibits nuclear pore formation and nuclear transport of a nuclear reporter protein. Eur. J. Biol. 59, 280-295.
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Nuclear transport and nuclear pores in yeast. Int. J. Gen. Mol. Microbiol. 62, 3-14.
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The nuclear envelope of the yeast Saccharomyces cerevisiae. Int. Rev. Cytol. 136, 145-184.
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Across the nuclear pores with the help of nucleoporins. Chromosoma 101, 199-205.
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Evolutionary conservation of the human protein fibrillarin and its functional expression in yeast. J. Cell Biol. 113, 715-727.
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Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur. J. Cell Biol. 55, 17-30.
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Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery. EMBO J. 10, 195-206.
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The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J. 10, 573-583.
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NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell 61, 979-989.
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Targeting of a cytosolic protein to the nuclear periphery. J. Cell Biol. 111, 2829-2837.
E.C. Hurt (1990)
The role of small nucleolar ribonucleoproteins in ribosome biogenesis. Mol. Biol. Report 14, 103-206.
D. Tollervey, and E. C. Hurt (1990)
A 40 kDa nuclear membrane protein changes its concentration and localization within the nuclear envelope of the yeast Saccharomyces cerevisiae in a cell cycle dependent manner. Eur. J. Cell Biol. 49, 33-41.
T. Schimmang and E. C. Hurt (1989)
A yeast nucleolar protein related to human fibrillarin is associated with small nucleolar RNA and essential for viability. EMBO J. 8, 4015-4025.
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NSP1, a yeast protein located at the nuclear periphery is required for the cell-cycle of Saccharomyces cerevisiae. J. Cell Sci. 12, 243-252.
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A novel nucleosceletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J. 7, 4323-4334.
E.C. Hurt (1988)
Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae. Eur. J. Cell Biol. 46, 554-563.
E.C. Hurt, A. McDowell, and T. Schimmang (1988)
Global Transcriptome Characterization and Assembly of the Thermophilic Ascomycete Chaetomium thermophilum. Genes 12, 1549. https://doi.org/10.3390/genes12101549 Singh, A., Schermann, G., Reislöhner, S., Kellner, N., Hurt, E., & Brunner, M. (2021)
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Cheng J, Lau B, La Venuta G, Ameismeier M, Berninghausen O, Hurt E, Beckmann R. (2020)
Construction of the Central Protuberance and L1 stalk during 60S subunit biogenesis Mol. Cell, doi.org/10.1016/j.molcel.2020.06.032.
Lukas Kater, Valentin Mitterer, Matthias Thoms, Jingdong Cheng, Otto Berninghausen, Roland Beckmann and Ed Hurt (2020)
Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome. Mol. Cell .
Benjamin Lau, Jingdong Cheng, Dirk Flemming, Giusseppe La Venuta, Otto Berninghausen, Roland Beckmann and Ed Hurt (2020)
Transformation of Chaetomium thermophilum and affinity-purification of native thermostable protein complexes. Methods in Mol. Biol., in press.
Nikola Kellner and Ed Hurt (2020)
Crystal structures of Rea1-MIDAS bound to its ribosome assembly factor ligands resembling integrin–ligand-type complexes. Nat Commun 10, 3050. https://doi.org/10.1038/s41467-019-10922-6 Ahmed, Y.L., Thoms, M., Mitterer, V., Sinning, I., & Hurt, E. (2019)
Eucaryotic Ribosome Assembly. Annu Rev Biochem. 2019 Jun 20;88:281-306. doi: 10.1146/annurev-biochem-013118-110817.
Baßler J, Hurt E. (2019)
Nucleoporin Nup155 is part of the p53 network in liver cancer. Mol Cell. 2019 Sep 19;75(6):1256-1269.e7. doi: 10.1016/j.molcel.2019.06.032.
Holzer K, Ori A, Cooke A, Dauch D, Drucker E, Riemenschneider P, Andres-Pons A, DiGuilio AL, Mackmull MT, Baßler J, Roessler S, Breuhahn K, Zender L, Glavy JS, Dombrowski F, Hurt E, Schirmacher P, Beck M, Singer S (2019)
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration. Mol Cell. 2019 Sep 19;75(6):1256-1269.e7. doi: 10.1016/j.molcel.2019.06.032.
Cheng J, Baßler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E. (2019)
Assembly Kinetics of Vimentin Tetramers to Unit-Length Filaments: A Stopped-Flow Study. Biophys J. 2018 May 22;114(10):2408-2418. doi: 10.1016/j.bpj.2018.04.032.
N. Mücke, L. Kämmerer, S. Winheim, R. Kirmse, J. Krieger, M. Mildenberger, J. Baßler, E. Hurt, WH Goldmann, U. Aebi, K. Toth, J. Langowski, H. Hermann (2018)
Structure of the nuclear exosome captured on a maturing preribosome. Science. 2018 Apr 13;360(6385):219-222. doi: 10.1126/science.aar5428.
Jan Schuller, Sebastian Falk, Lisa Fromm, Ed Hurt and Elena Conti (2018)
Suppressor mutations in Rpf2-Rrs1 or Rpl5 bypass the Cgr1 function for pre-ribosomal 5S RNP-rotation. Nat Commun. 2018 Oct 5;9(1):4094. doi: 10.1038/s41467-018-06660-w.
Matthias Thoms, Valentin Mitterer, Lukas Kater, L. Falquet, Roland Beckmann, Dieter Kressler, Ed Hurt (2018)
3.2 Angstrœm resolution structure of the 90S pre-ribosome before A1 pre-rRNA cleavage. Nat. Struct. Mol. Biol. doi:10.1038/nsmb.3476.
Jingdong Cheng, Nikola Kellner, Otto Berninghausen, Ed Hurt and Roland Beckmann (2017)
A puzzle of life: crafting ribosomal subunits Trends Biochem. Sci. , in press.
Dieter Kressler, Ed Hurt and Jochen Bassler (2017)
Cryo-EM structure of a late pre-40S ribosomal subunit from Saccharomyces cerevisiae. eLife, 6, e30189. https://doi.org/10.7554/eLife.30189 André Heuer, Emma Thomson, Christian Schmidt, Otto Berninghausen, Thomas Becker, Ed Hurt and Roland Beckmann (2017)
Eukaryotic ribosome assembly, transport and quality control. Nat. Struct. Mol. Biol. 24, 689-699. doi: 10.1038/nsmb.3454.
Cohue Peña, Ed Hurt and Vikram Govind Panse (2017)
Interaction network of the ribosome assembly machinery from a eukaryotic thermophile. Protein science 26(2), 327–342. https://doi.org/10.1002/pro.3085 Baßler, J., Ahmed, Y.L., Kallas, M., Kornprobst, M., Rodriguez-Calvino, F., Gnädig, M., Thoms, M., Stier, G., Ismail, S., Kharde, S., Castillo, N., Griesel, S., Bastuck, S., Bradatsch, B., Thomson, E., Flemming, D., Sinning, I.* & Hurt, E.* (2017)
Interdependent action of KH domain proteins Krr1 and Dim2 drive the 40S platform assembly. Nature Commun., in press.
Miriam Sturm, Jingdong Cheng, Jochen Bassler, Roland Beckmann and Ed Hurt (2017)
Mpp10 represents a platform for the interaction of multiple factors within the 90S pre-ribosome, PlosOne 12: e0183272. Sá-Moura, B., Kornprobst, M., Kharde, S., Ahmed, Y.L., Stier, G., Kunze, R., Sinning*, I. & Hurt*, E. (2017)
Pre-ribosomes escaping the nucleus are caught during translation by cytoplasmic quality control quality control mechanisms. Nat. Struct. Mol. Biol., doi: 10.1038/nsmb.349.
Anshuk Sarkar, Matthias Thoms, Clara Barrio-Garcia, Emma Thomson, Dirk Flemming, Roland Beckmann and Ed Hurt (2017)
Reconstitution of the complete pathway of of ITS2 processing at the pre-ribosome. Nature Commun., in press.
Lisa Fromm, Sebastian Falk, Dirk Flemming, Jan Schuller, Matthias Thoms, Elena Conti and Ed Hurt (2017)
Short linear motifs in scaffold Nups act as sensors to cooperatively connect pre-assembled modules during NPC biogenesis. Nature Commun. 8, 1107. doi: 10.1038.
Roman Teimer, Jan Kosinski, Alexander von Appen, Martin Beck and Ed Hurt (2017)
Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis. PLOS ONE 12(6): e0178752. https://doi.org/10.1371/journal.pone.0178752 Calviño, F.R., Kornprobst, M., Schermann, G., Birkle, F., Wild, K., Fischer, T., Hurt, E., Ahmed, Y.L. & Sinning, I. (2017)
Structural insights into the interaction of the nuclear exosome helicase Mtr4 with the pre-ribosomal protein Nop53. RNA 23, 1780-1787. doi: 10.1261/rna.062901.117.
Sebastian Falk, Jan-Niklas Tants, Ed Hurt, Michael Sattler and Elena Conti (2017)
The nuclear pore complex: understanding its function through structural insight. Nature Rev. Mol. Cell Biol. 18, 73-89.
Martin Beck and Ed Hurt (2017)
Visualizing the assembly pathway of nucleolar pre-60S ribosomes, Cell 171: 1599-1610. Kater, L., Thoms, M., Barrio Garcia, C., Cheng, J., Ismail, S., Ahmed, Y.L., Bange, G., Kressler, D., Berninghausen, O., Sinning, I., Hurt, E. & Beckmann, R. (2017)
Visualizing the earliest steps during nucleolar pre-60S ribosome assembly. Cell 171, 1599–1610. Lukas Kater, Matthias Thoms, Clara Barrio Garcia, Jingdong Cheng, Sherif Ismail, Yasar Luqman Ahmed, Gert Bange, Dieter Kressler, Otto Berninghausen, Irmi Sinning, Ed Hurt and Roland Beckmann (2017)
Architecture of a ribosome assembly bound to a combined remodeling-checkpoint machinery. Nature Struct. Mol. Biol. 23, 37-44 doi:10.1038/nsmb.3132.
Clara Barrio-Garcia, Matthias Thoms, Dirk Flemming, Lukas Kater, Jochen Bassler, Roland Beckmann and Ed Hurt (2016)
Architecture of the 90S pre-ribosome: a structural view on the birth of the eukaryotic ribosome. Cell 166, 380–393. Markus Kornprobst, Martin Turk, Nikola Kellner, Jing-Dong Cheng, Dirk Flemming, Martin Koš, Isabelle Ko&scaron, Matthias Thoms, Otto Berninghausen, Roland Beckmann and Ed Hurt (2016)
Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome. Cell, 166(2), 380–393. https://doi.org/10.1016/j.cell.2016.06.014 Kornprobst, M., Turk, M., Kellner, N., Cheng, J., Flemming, D., Koš-Braun, I., Koš, M., Thoms, M., Berninghausen, O., Beckmann, R., & Hurt, E. (2016)
Concerted removal of the Erb1-Ytm1 complex in ribosome biogenesis relies on an elaborate interface, Nucl. Acids Res. 44: 926-39. Thoms, M., Ahmed. Y.L., Maddi, K., Hurt, E. & Sinning, I. (2016)
Coupling ribosome stalk biogenesis with nuclear export factor recruitment to the nascent 60S subunit. Nat. Struct. Mol. Biol. 23, 1074-1082.
Anshuk Sarkar, Markus Pech, Matthias Thoms, Dirk Flemming, Roland Beckmann and Ed Hurt (2016)
Developing genetic tools to exploit Chaetomium thermophilum for biochemical analyses of eukaryotic macromolecular assemblies. Sci Rep. 5:15401. doi: 10.1038/srep15401.
Nikola Kellner, Johannes Schwarz, Miriam Sturm, Javier Fernandez-Martinez, Sabine Griesel, Wenzhu Zhang, Brian Chait, Michael P. Rout, Ulrich Kück and Ed Hurt (2016)
Molecular architecture of the inner ring scaffold of the human nuclear pore complex. Science 352, 363-365. doi: 10.1126/science.aaf0643.
Jan Kosinski, Shyamal Mosalaganti, Alexander von Appen, Roman Teimer, Amanda DiGuilio, William Wan, Huy Khahn Bui, Wim J.H. Hagen, John A.G. Briggs, Joseph S. Glavy, Ed Hurt and Martin Beck (2016)
Role of cation-dependent Nug1 GTPase in recruiting Dbp10 helicase to the undeveloped peptidyl transferase center of the pre-ribosome. Nucl Acid Res. doi: 10.1093/nar/gkw045.
George Manikas, Emma Thomson and Ed Hurt (2016)
A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation, J. Cell Biol. 210: 169-70. Bassler, J., Paternoga, H., Holdermann, I., Thoms, M., Grannemann, S., Barrio-Garcia, C., Nyarko, A., Lee, W. Stier, G., Clark, S., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning*, I. & Hurt*, E. (2015)
Architecture of a ribosome assembly bound to a combined remodeling-checkpoint machinery. Nature Struct. Mol. Biol.
Clara Barrio-Garcia, Matthias Thoms, Dirk Flemming, Lukas Kater, Jochen Bassler, Roland Beckmann and Ed Hurt (2015)
Architecture of the nuclear pore inner ring complex. Science 350, 56-64.
Tobias Stuwe, Christopher J. Bley, Karsten Thierbach, Stefan Petrovic, Sandra Schilbach, Daniel J. Mayo, Thibaud Perriches, Emily J. Rundlet, Young Jeon, Leslie N. Collins, Daniel H. Lin, Marcin Paduch, Akiko Koide, Vincent Lu, Jessica Fischer, Ed Hurt, Shohei Koide, Anthony A. Kossiakoff, André Hoelz (2015)
Characterization of the Chaetomium thermophilum TREX-2 complex and its interaction with the mRNA nuclear export factor MEX67:MTR2. Structure 23, 1246-1257.
Lyudmila Dimitrova, Eugine Valkow, Shinataro Aibara, Dirk Flemming, Steven McLaughlin, Ed Hurt and Murray Stewart (2015)
Coordinated ITS2 rRNA processing by the Las1 complex integrating endonuclease, polynucleotide kinase and exonuclease activities Mol. Cell, 60(5):808-15. doi: 10.1016/j.molcel.2015.10.021.
Lisa Gasse, Dirk Flemming and Ed Hurt (2015)
Coordinated ribosomal L4 protein assembly into the pre-ribosome is regulated by its eukaryote-specific extension. Mol. Cell 58, 854-862.
Philipp Stelter, Ferdinand Huber, Ruth Kunze, Dirk Flemming, André Hoelz and Ed Hurt (2015)
Direct and high throughput (HT) interactions on the ribosomal surface by iRIA. Sci Rep. 5:15401. doi: 10.1038/srep15401. Elisa Pesce, Claudia Minici, Jochen Bassler, Ed Hurt, Massimo Degano, Piera Calamita and Stefano Biffo (2015)
Einblicke in die 'Kinderstube' während der Ribosomen-Entwicklung. Biospektrum (Springer Verlag) 02.15, 144-147.
Jochen Baßler und Ed Hurt (2015)
Linker Nups connect the nuclear pore complex inner ring with outer ring and transport channel. Nature Struct. Mol. Biol. 22, 774-81.
Jessica Fischer, Roman Teimer, Stefan Amlacher, Ruth Kunze and Ed Hurt (2015)
NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export. Nucl. Acid Res 3, 1894-904.
Jun Katahira, Lyudmila Dimitrova, Yumiko Imai, Ed Hurt (2015)
Structural basis for assembly and function Nup82 complex in the nuclear pore scaffold. J. Cell Biol. 208, 283-297 doi/10.1083/jcb.201411003.
Monika Gaik, Dirk Flemming, Alexander von Appen, Panagiotis Kastritis, Philipp Stelter, Khanh Huy Bui, Alessandro Ori, Jochen Bassler, Jessica Fischer, Elisar Barbar, Martin Beck and Ed Hurt (2015)
Structural characterization of the principal mRNA export factor Mex67:Mtr2 from Chaetomium thermophilum. Acta Cryst.F71, 876-888.
Shintaro Aibara, Eugene Valkov, Meindert Lamers, Lyudimila Dimitrova, Ed Hurt and Murray Stewart (2015)
Symportin 1 chaperones 5S RNP assembly during ribosome biogenesis by occupying an essential rRNA-binding site. Nat Commun 6, 6510. https://doi.org/10.1038/ncomms7510 Calviño, F.R., Kharde, S., Ori, A., Hendricks, A., Wild, K., Kressler, D., Bange, G., Hurt, E., Beck, M. & Sinning, I. (2015)
The exosome is recruited to RNA substrates through specific adapter proteins. Cell 162, 1029-1038.
Matthias Thoms, Emma Thomson Jochen Baßler, Marén Gnädig, Sabine Griesel and Ed Hurt (2015)
Toward understanding nuclear pore complex architecture and dynamics in the age of integrative structural analysis. Curr. Opin. Cell Biol. 34:31-38. doi: 10.1016/j.ceb.2015.04.009.
Ed Hurt und Martin Beck (2015)
60S ribosome biogenesis requires rotation of the 5S ribonucleoprotein particle, Nat. Commun. 5: 3491. doi: 10.1038/ncomms4491. Leidig, C., Thoms, M., Holdermann, I., Bradatsch, B., Berninghausen, O., Bange, G., Sinning, I., Hurt, E. & Beckmann, R. (2014)
A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation, J. Cell Biol. 207: 481-498. Bassler, J., Paternoga, H., Holdermann, I., Thoms, M., Grannemann, S., Barrio-Garcia, C., Nyarko, A., Stier, G., Clark, S., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning*, I. & Hurt*, E. (2014)
A pulse-chase epitope labeling to study cellular dynamics of newly synthesized proteins: a novel strategy to characterize NPC biogenesis and ribosome maturation/export,. Methods in Cell Biol. 122C,147-163. doi: 10.1016/B978-0-12-417160-2.00007-2 .
Philipp Stelter and Ed Hurt (2014)
An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum. Nucleic acids research, 42(22), 13525–13533. https://doi.org/10.1093/nar/gku1147 Bock, T., Chen, W. H., Ori, A., Malik, N., Silva-Martin, N., Huerta-Cepas, J., Powell, S. T., Kastritis, P. L., Smyshlyaev, G., Vonkova, I., Kirkpatrick, J., Doerks, T., Nesme, L., Baßler, J., Kos, M., Hurt, E., Carlomagno, T., Gavin, A. C., Barabas, O., Müller, C. W., van Noort, V., Beck, M., & Bork, P. (2014)
Catalytic Rio1 kinase/ATPase mutant induces trapping of late pre-40S biogenesis factors in 80S-like ribosomes. Nucl. Acid Res. 1, doi: 10.1093/nar/gku542 .
Sébastien Ferreira-Cerca, Irene Kiburu, Emma Thomson, Nicole Laronde- Leblanc and Ed Hurt (2014)
Coupled GTPase and remodeling ATPase activities form a checkpoint for ribosome export. Nature 505, 112-116 .
Yoshitaka Matsuo, Sander Granneman, Matthias Thoms, Rizos-Georgios Manikas, David Tollervey and Ed Hurt (2014)
Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192 and karyopherins. Proc. Natl. Acad. Sci. USA 18, 2530-2535 .
Tobias Stuwe, Daniel H. Lin, Leslie Collins, Ed Hurt and André Hoelz (2014)
Functional reconstitution of de novo mitochondrial Fe/S cluster synthesis reveals an essential role of ferredoxin. Nature Com. 5, doi:10.1038/ncomms6013 .
Stefan Amlacher, Ed Hurt, Ulrich Mühlenhoff, Lucia Banci and Roland Lill (2014)
Functional reconstitution of mitochondrial Fe/S cluster synthesis on Isu1 reveals the involvement of ferredoxin. Nat Commun 5, 5013. https://doi.org/10.1038/ncomms6013 Webert, H., Freibert, S.-A., Gallo, A., Heidenreich, T., Linne, U., Amlacher, S., Hurt, E., Mühlenhoff, U., Banci, L., & Lill, R. (2014)
Utilizing the Dyn2-dimerization- zipper as a tool to probe NPC structure and function. Methods in Cell Biol. 122C, 99-115. doi: 10.1016/B978-0-12-417160-2.00005-9 .
Dirk Flemming, Philipp Stelter and Ed Hurt (2014)
Consistent mutational paths predict eukaryotic thermostability. BMC evolutionary biology, 13, 7. https://doi.org/10.1186/1471-2148-13-7 van Noort, V., Bradatsch, B., Arumugam, M., Amlacher, S., Bange, G., Creevey, C., Falk, S., Mende, D. R., Sinning, I., Hurt, E., & Bork, P. (2013)
Eukaryotic Ribosome Biogenesis at a glance. J. Cell Sci. in press. https://doi.org/10.1242/jcs.111948 Emma Thomson, Sébastien Ferreira-Cerca and Ed Hurt (2013)
New twist to nuclear import: When two travel together, Com. & Int. Biol. 6:4, e24792; July/August 2013, Landes Bioscience). Bange, G. , Murat, G., Sinning, I., Hurt, E. & Kressler, D. (2013)
Protein interfaces of the Conserved Nup84 Complex from Chaetomium thermophilum revealed by cross-linking mass spectrometry and electron microscopy. Structure 21, 9, 1672-1682.
Karsten Thierbach, Alexander von Appen, Matthias Thoms, Martin Beck, Dirk Flemming and Ed Hurt (2013)
Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae. Nucl. Acid Res. 41, 1191-1200.
Thomas Hierlmeier, Juliane Merl, Martina Sauert, Jorge Perez-Fernandez, Patrick Schultz, Astrid Bruckmann, Stephan Hamperl, Uli Ohmayer, Joachim Griesenbeck, Reinhard Rachel, Anja Jakob, Kristin Hergert, Rainer Deutzmann, Ed Hurt, Philipp Milkereit, Jochen Baßler and Herbert Tschochner (2013)
Structural basis of histone H2A-H2B recognition by the essential chaperone FACT. Nature 499, 111-114.
Maria Hondele, Tobias Stuwe, Markus Hassler, Felix Halbach, Bianca Nijmeijer, Vladimir Rybin, Stefan Amlacher, Ed Hurt and Andreas Ladurner (2013)
Structural characterization of a eukaryotic co-translational chaperone, the ribosome-associated complex (RAC), Nat. Struct. Mol. Biol. 20:23-8. Leidig, C., Bange, G., Kopp, J., Amlacher, S., Aravind, A., Wickles, S., Witte, G., Hurt, E., Beckmann, R. & Sinning, I. (2013)
Structures of the nuclear export receptor CRM1 in its free state reveal major structural rearrangements upon cargo binding. Proc. Natl. Acad. Sci. USA 110, 960-965 .
Thomas Monecke, David Haselbach, Piotr Neuman, Emma Thomson, Ed Hurt, Holger Stark, Achim Dickmanns and Ralf Ficner (2013)
Analysis of the yeast nucleoporin Nup188 reveals a conserved S-like structure with similarity to karyopherins. J. Struct. Biol. 177, 99-105.
Dirk Flemming, Damien Devos, Johannes Schwarz, Stefan Amlacher, Malik Lutzmann and Ed Hurt (2012)
ATPase-dependent role of the atypical kinase Rio2 on the evolving pre-40S ribosomal subunit. Nat. Struct. Mol. Biol. 19, 1316-1323.
Sébastien Ferreira-Cerca, Vatsala Sagar, Thorsten Schäfer, Anne-Maria Wesseling, Eileen Chai, Ed Hurt and Nicole LaRonde-LeBlanc (2012)
Functional and structural characterisation of the mammalian TREX-2 complex that links transcription with nuclear mRNA export. Nucl. Acid Res. 40, 4562-4573.
Divyang Jani, Sheila Lutz, Ed Hurt, Ronald Laskey, Murray Stewart and Vihandha Wickramasinghe (2012)
Monitoring Spatiotemporal Biogenesis of Macromolecular Assemblies by Pulse-chase Epitope Labeling. Mol. Cell 47, 788-796.
Matthias Thoms and Ed Hurt (2012)
Monitoring spatiotemporal biogenesis of macromolecular assemblies by pulse-chase epitope labeling. Molecular cell, 47(5), 788–796. https://doi.org/10.1016/j.molcel.2012.06.015 Stelter, P., Kunze, R., Radwan, M., Thomson, E., Thierbach, K., Thoms, M., & Hurt, E. (2012)
Structural basis for the assembly and nucleic acid binding of the TREX-2 transcription-export complex. Nat. Struct. Mol. Biol. 19, 328-336.
Andrew M. Ellisdon, Lyudmila Dimitrova, Ed Hurt and Murray Stewart (2012)
Structure of the pre-60S ribosomal subunit with nuclear export factor Arx1 bound at the exit tunnel. Nat. Struct. Mol. Biol 19, 1234-1241.
Bettina Bradatsch, Christoph Leidig, Sander Granneman, Marén Gnädig, Bettina Böttcher, David Tollervey, Roland Beckmann and Ed Hurt (2012)
Synchronizing nuclear import of ribosomal proteins with ribosome assembly, Science 338: 666-71. Kressler*, D., Bange, G. Ogawa, Y., Bradatsch, B., Pratte, D., Strauß, D., Amlacher, S., Yoneda,Y., Katahira, J., Sinning, I.*, & Hurt, E.* (2012)
The conserved Bud20 zinc finger protein is a new component of the ribosomal 60S subunit export machinery. Mol. Cell Biol., 32, 4898-912.
Jochen Baßler, Isabella Klein, Claudia Schmidt, Emma Thomson, Martina Kallas, Maria Anna Wagner, Gerald Rechberger, Heimo Strohmaier, Ed Hurt,Helmut Bergler (2012)
The power of AAA- ATPases on the road of pre-60S ribosome maturation - molecular machines that strip pre-ribosomal particles. Biochim. Biophys. Acta1823, 92-100.
Dieter Kressler, Ed Hurt, Helmut Bergler and Jochen Baßler (2012)
Bange, Nico Kümmerer, Przemyslaw Grudnik, Robert Lindner, Georg Petzold, Dieter Kressler, Ed Hurt, Klemens Wild and Irmgard Sinning (2012) Structural basis for the molecular evolution of SRP-GTPase activation by protein. Nature Struct. Mol. Biol. 18, 1376-1380.
Dieter Kressler, Ed Hurt, Helmut Bergler and Jochen Baßler (2011)
Insight into structure and assembly of the nuclear pore complex by utilizing the genome of a eukaryotic thermophile. Cell 146, 277-289, July 22.
Stefan Amlacher, Phillip Sarges, Dirk Flemming, Vera van Noort, Ruth Kunze, Damien Devos, Manimozhiyan Arumugam, Peer Bork and Ed Hurt (2011)
Linking gene regulation to mRNA production and export. Curr. Opinion Cell Biol. 23, 302-309.
Susana Rodriguez-Navarro and Ed Hurt (2011)
Probing the FG repeat network of nucleoporins defines structural and functional features of the nuclear pore complex. J. Cell Biol. 195, 183-192.
Philipp Stelter, Ruth Kunze, Jessica Fischer and Ed Hurt (2011)
Structural basis for the molecular evolution of SRP-GTPase activation by protein. Nat Struct Mol Biol. 8:1376-80. Bange G, Kümmerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I. (2011)
Driving Ribosome Assembly. Biochim. Biophys. Acta 1803, 673-683.
Dieter Kressler, Ed Hurt and Jochen Bassler (2010)
Gene regulation by nucleoporins and links to cancer. Mol. Cell 38, 6-15.
Alwin Köhler and Ed Hurt (2010)
Precise mapping of subunits in multiprotein complexes by a versatile EM-label. Nature Struct. Mol. Biol. in press.
Dirk Flemming, Karsten Thierbach, Philipp Stelter, Bettina Boettcher and Ed Hurt (2010)
Structural basis for assembly and activation of the heterotetrameric SAGA histone H2B deubiquitinase module. Cell 141, 606-617.
Alwin Köhler, Eric Zimmermann, Maren Schneider, Ed Hurt and Ning Zheng (2010)
Structural basis for the interaction between yeast Spt-Ada-Gcn5 acetyltransferase (SAGA) complex components Sgf11 and Sus1. J. Biol. Chem. 285, 3850-3856.
Andrew M. Ellisdon, Divyang Jani, Alwin Köhler, Ed Hurt and Murray Stewart (2010)
The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly. Mol. Cell in press.
Jochen Baßler, Martina Kallas, Matthias Thoms, Cornelia Ulbrich, Brigitte Pertschy and Ed Hurt (2010)
The structure of Get4 reveals an alpha-solenoid fold adapted for multiple interactions in tail-anchored protein biogenesis. FEBS Lett. 584(8):1509-14. Bozkurt G, Wild K, Amlacher S, Hurt E, Dobberstein B, Sinning I. (2010)
A toxic nucleoporin mutant induces abarrent nucleoporin foci in the cytoplasm. J. Cell Biol. 185, 387-396.
Dirk Flemming, Philipp Sarges, Philipp Stelter, Andrea Hellwig, Bettina Boettcher and Ed Hurt (2009)
An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes. PLoS Biology 7 e8.
Michal Skruzny, Claudia Schneider, Attila Racz, Julan Weng, David Tollervey and Ed Hurt (2009)
Arrest by Ribosome. Nature 459, 46-47.
Sébastien Ferreira-Cerca and Ed Hurt (2009)
Linear ubiquitin fusion to Rps31 and its subsequent cleavage 1 are required for the efficient production and functional integrity of 40S ribosomal subunits. Mol. Microbiol. 72, 69-84.
Thierry Lacombe, Juan J. García-Gómez, Jesús de la Cruz, Daniela Roser, Ed Hurt, Patrick Linder and Dieter Kressler (2009)
Mechanochemical Removal of Ribosome Biogenesis Factors from Nascent 60S Ribosomal Subunits. Cell 138, 911-922.
Cornelia Ulbrich, Meikel Diepholz, Jochen Baßler, Dieter Kressler, Brigitte Pertschy, Kiki Galani, Bettina Böttcher and Ed Hurt (2009)
Mutational Uncoupling of Sus1's role in NPC-targeting of an mRNA Export Complex and Histone H2B deubiquitination J. Biol. Chem. 284, 12049-12056.
Christoph Klöckner, Maren Schneider, Sheila Lutz, Dieter Kressler, Divyang Jani, Murray Stewart, Ed Hurt and Alwin Köhler (2009)
Nucleus-Specific and Cell Cycle-Regulated Degradation of MAPK Scaffold Protein Ste5 Contributes to the Control of Signaling Competence. Mol. Cell Biol. 29, 582-601.
Lindsay S. Garrenton, Andreas Braunwarth, Stefan Irniger, Ed Hurt, Markus Künzler and Jeremy Thorner (2009)
Purification of poly(A)-binding protein Nab2 reveals association with the yeast transcriptome and a messenger ribonucleoprotein (mRNP) core structure. J.Biol.Chem. 284, 354911-354917.
Julien Batisse, Claire Batisse, Aidan Budd, Bettina Böttcher and Ed Hurt (2009)
RNA helicase Prp43 and its co-factor Pfa1 promote 20S to 18S rRNA processing catalyzed by the endonuclease Nob1. J.Biol.Chem. 284, 35079-35091.
Brigitte Pertschy, Claudia Schneider, Maren Gnädig, Thorsten Schäfer, David Tollervey and Ed Hurt (2009)
Sem1 is a functional component of the nuclear pore complex associated mRNA export machinery. J. Cell Biol. 184, 833-846.
Marius Boulos Faza, Stefan Kemmler, Sonia Jimeno, Christina González-Aguilera, Andres Aguilera, Ed Hurt and Vikram Govind Panse (2009)
Structural basis for the Sus1:Sgf11 interaction in the yeast SAGA transcription activation complex. J. Biol. Chem. in press.
Andrew M. Ellisdon, Divyang Jani, Alwin Köhler, Ed Hurt and Murray Stewart (2009)
Structural insights into tail-anchored protein binding and membrane insertion by Get3. Proc Natl Acad Sci U S A. 106(50):21131-6. Bozkurt G, Stjepanovic G, Vilardi F, Amlacher S, Wild K, Bange G, Favaloro V, Rippe K, Hurt E, Dobberstein B, Sinning I. (2009)
Sus1, Cdc31, and the Sac3 CID region form a conserved interaction platform that promotes nuclear pore association and mRNA export. Mol Cell 33, 727-737. Jani, D., Lutz, S., Marshall, N.J., Fischer, T., Kohler, A., Ellisdon, A.M., Hurt, E., and Stewart, M. (2009)
The adaptor Aly and co-adaptor Thoc5 function in Tap-p15 mediated nuclear export of HSP70 mRNA. EMBO J. 28, 556-567.
Jun Katahira, Hitomi Inoue, Ed Hurt, Yoshihiro Yoneda (2009)
A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export. EMBO J. 27, 6–16.
Wei Yao, Malik Lutzmann and Ed Hurt (2008)
Der Kernporenkomplex oder das Tor zur Welt des Zytoplasmas. Im Jahrbuch 2007 der Deutschen Akademie der Naturforscher Leopoldina. Leopoldina 53, 457-460.
Ed Hurt (2008)
Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proc Natl Acad Sci U S A 105, 11731-11736. → Highlighted by the Faculty of 1000 (F1000.com/1119165). http://www.pnas.org/content/105/33/11731 Beck, R, Sun, Z, Adolf, F, Rutz, C, Bassler, J, Wild, K, Sinning, I, Hurt, E, Brügger, B, Béthune, J*, and Wieland, F* (2008)
Structural basis of the Nic96 subcomplex organization in the nuclear pore channel. Mol. Cell 29, 46-55.
Nils Schrader, Philipp Stelter, Dirk Flemming, Ruth Kunze, Ed Hurt* and Ingrid Vetter* (*corresponding authors) (2008)
The AAA-ATPase Rix7 powers progression of ribosome biogenesis by stripping Nsa1 from pre-60S particles. J. Cell Biol. 181, 835-844.
Dieter Kressler, Daniela Roser, Brigitte Pertschy and Ed Hurt (2008)
The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression. J Cell Biol 182, 897-910. Grund, S.E., Fischer, T., Cabal, G.G., Antunez, O., Perez-Ortin, J.E., and Hurt, E. (2008)
Yeast Ataxin-7 links histone deubiquitination with gene gating and mRNA export. Nat. Cell Biol. 10, 707-715.
Alwin Köhler, Maren Schneider, Ghislain Cabal, Ulf Nehrbass and Ed Hurt (2008)
Adjunct duties for karyopherins: Regulating septin sumoylation. Dev. Cell 12, 669-670.
Vikram Govind Panse and Ed Hurt (2007)
Arx1 functions as alternative nuclear export receptor for the 60S pre-ribosomal subunit. Mol. Cell 27, 767-779.
Bettina Bradatsch, Jun Katahira, Eva Kowalinski, Gert Bange, Wei Yao, Toshihiro Sekimoto, Viola Baumgärtel, Guido Boese, Jochen Bassler, Klemens Wild, Reiner Peters, Yoshihiro Yoneda, Irmi Sinning and Ed Hurt (2007)
Exporting RNA from the nucleus to the cytoplasm. Nature Rev. Mol. Cell Biol. 8, 761-773.
Alwin Köhler and Ed Hurt (2007)
Molecular basis for the functional interaction of dynein light chain with the nuclear pore complex. Nature Cell Biology 9, Number 7, 788-796.
Philipp Stelter, Ruth Kunze, Dirk Flemming, Dominic Höpfner, Meikel Diepholz, Peter Philipsen, Bettina Böttcher and Ed Hurt (2007)
Nuclear export of ribosomal 60S subunits by the general mRNA export receptor Mex67-Mtr2. Mol. Cell 26, 51-62.
Wei Yao, Daniela Roser, Alwin Köhler, Bettina Bradatsch, Jochen Baßler and Ed Hurt (2007)
The crystal structure of Ebp1 reveals how the methionine aminopeptidase fold serves as a binding platform for multiple interactions. FEBS Lett. 581, 4450-4454.
Eva Kowalinski, Gert Bange, Bettina Bradatsch, Ed Hurt, Klemens Wild and Irmi Sinning (2007)
A round of phosphorylation/de-phosphorylation triggers maturation of the pre-ribosomal 40S subunit. Nature 441, 651-655.
Thorsten Schäfer, Bohumil Maco, Elisabeth Petfalski, David Tollervey, Bettina Boettcher, Ueli Aebi and Ed Hurt (2006)
Coordinated nuclear import of RNA polymerase III subunits. Traffic 7, 465-473.
Ulrike Hardeland and Ed Hurt (2006)
Formation and nuclear export of pre-ribosomes are functionally linked to the small-ubiquitin-related (SUMO) modifier pathway. Traffic 7, 1311-1321.
Vikram Govind Panse, Dieter Kressler, Andrea Pauli, Elisabeth Petfalski, Marén Gnädig, David Tollervey and Ed Hurt (2006)
Molecular Analysis of SAGA Mediated Gene Gating In Yeast. Nature 441, 770-773.
Ghislain Cabal, Susana Rodrigez-Navarro, August Genevesio, Jean-Christophe Olivo-Marin, Christophe Zimmer, Olivier Gadal, Frank Feuerbach-Fournier, Annick Lesne, Henri Buc, Ed Hurt* and Ulf Nehrbass* (*corresponding authors) (2006)
The mRNA Export Factor Sus1 Is Involved in SAGA-mediated H2B Deubiquitinylation through Its Interaction with Ubp8 and Sgf11. Mol. Biol. Cell. 17, 4228-4236.
Alwin Köhler, Pau Pascual-García, Ana Llopis, Meritxell Zapater, Francesc Posas, Ed Hurt and Susana Rodríguez-Navarro (2006)
The Nug1 GTPase reveals an N-terminal RNA binding domain that is essential for association with pre-60S subunits. J. Biol. Chem. 281, 24737-24744.
Jochen Baßler, Martina Kallas and Ed Hurt (2006)
TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients. EMBO J. 25, 3832-3842.
Yoshimi Honma, Aiko Kitamura, Ryo Shioda, Hironori Maruyama, Kanako Ozaki, Yoko Oda, Thierry Mini, Paul Jen, Yasushi Maki, Kazuhito Yonezawa, Ed Hurt, Masaru Ueno, Masahiro Uritani, Michael N. Hall, and Takashi Ushimaru (2006)
Functional link between ribosome formation and biogenesis of iron-sulfur proteins. EMBO J. 24, 580-588.
Alexander Yarunin, Vikram Panse, Elisabeth Petfalski, Christophe Dez, David Tollervey and Ed Hurt (2005)
Reconstitution of Nup157 and Nup145N into the Nup84 complex. J. Biol. Chem. 280, 18442-18451.
Malik Lutzmann, Ruth Kunze, Karin Stangl, Philipp Stelter, Katalin Fejes Tóth, Bettina Boettcher and Ed Hurt (2005)
Recruitment of the human TREX complex to mRNA during splicing Genes and Develop.19, 1512-1517.
Seiji Masuda, Rita Das, Ed Hurt, Nijsje Dorman and Robin Reed (2005)
The tRNA aminoacylation co-factor Arc1p is excluded from the nucleus by an Xpo1p-dependent mechanism. FEBS Lett. 579, 969-975.
Kyriaki Galani, Eduard C. Hurt and George Simos (2005)
A nascent pre-ribosome with a tadpole-like structure functions in ATP-dependent maturation of 60S subunits. Mol. Cell 15, 295-301.
Tracy A. Nissan, Kyriaki Galani, Bohumil Maco, David Tollervey, Ueli Aebi and Ed Hurt (2004)
A proteome-wide approach identifies sumolyated substrate proteins in yeast. J. Biol. Chem., in press.
Vikram Govind Panse, Ulrike Hardeland, Thilo Werner, Bernhard Kuster and Ed Hurt (2004)
Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. PNAS Vol. 101, no. 7, 1858-1862.
Ed Hurt, Ming-juan Luo, Susanne Röther, Robin Reed and Katja Sträßer (2004)
Rea1, a dynein-related nuclear AAA-type ATPase, is involved in late 25S rRNA processing and nuclear export of 60S subunits. J. Biol. Chem. 279, 55411-55418.
Kyriaki Galani, Tracy A. Nissan, Elisabeth Petfalski, David Tollervey and Ed Hurt (2004)
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Cell. 116(1):75-86. Rodriguez-Navarro S, Fischer T, Luo MJ, Antunez O, Brettschneider S, Lechner J, Perez-Ortin JE, Reed R, Hurt E. (2004)
The Yeast Kinase Swe1 is Required for proper Entry into a new Cell Cycle after Arrest due to Ribosome Biogenesis Defects. Cell Cycle 3, 648-54.
Francesca Saracino, Jochen Baßler, Diego Muzzini, Ed Hurt and Maria Luisa Agostoni Carbone (2004)
Yeast centrin Cdc31 is linked to the nuclear mRNA export machinery. Nat. Cell Biol. 6, 840—848. Tamás Fischer, Susana Rodriguez-Navarro, Elmar Schiebel and Ed Hurt (2004)
A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits. The Journal of Biological Chemistry, Vol. 278, No. 6, pp. 4072-4081. Milkereit, P., Strauss, D., Bassler, J., Gadal, O., Kühn, H., Schütz, S., Gas, N., Lechner, J., Hurt, E., & Tschochner, H. (2003)
Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 4(12):1156-62. Neumann S, Petfalski E, Brügger B, Grosshans H, Wieland F, Tollervey D, Hurt E. (2003)
Identification and characterization of a novel RanGTP binding protein Yrb30. J. Biol. Chem. 278, 15397-15405. Andreas Braunwarth, Michel Fromont-Racine, Pierre Legrain, Ralf Bischoff, Thomas Gerstberger, Ed Hurt and Markus Künzler (2003)
Pre-Ribosomes on the Road from the Nucleolus to the Cytoplasm. Trends Cell Biol. 13, 255-263. Herbert Tschochner and Ed Hurt (2003)
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes. EMBO J. 22, 1370-1380.
Thorsten Schäfer, Daniela Strauß, Elisabeth Petfalski, David Tollervey and Ed Hurt (2003)
Unconventional tethering of Ulp1 to the transport channel of the nuclear pore complex by karyopherins. Nature Cell Biology, Vol. 5, 21-27.
Panse V., Küster B., Gerstberger Th., Hurt E. (2003)
60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm. EMBO J. 2002 Oct 15; 21(20):5539-47.
Nissan T., Bassler J., Petfalski E., Tollervey D., Hurt E. (2002)
90S pre-ribosomes include the 35S pre-rRNA, the U3 snoRNP, and 40S subunit processing factors but predominantly lack 60S synthesis factors. Mol Cell. 2002 Jul; 10(1):105-15.
Grandi P., Rybin V., Bassler J., Petfalski E., Strauss D., Marzioch M., Schaefer T., Kuster B., Tschochner H., Tollervey D., Gavin AC., Hurt E. (2002)
A conserved mRNA export machinery coupled to pre-mRNA splicing. Cell 108, 523-531.
Robin Reed and Ed Hurt (2002)
An intron in the YRA1 gene is required to control Yra1p protein expression and mRNA export. EMBO Rep. 3, 438-442.
Susana Rodríguez-Navarro, Katja Sträßer and Ed Hurt (2002)
Complex Formation between Tap and p15 Affects Binding to FG-repeat Nucleoporins and Nucleocytoplasmic Shuttling. J. Biol. Chem., 11, No. 11, March, 9242-9246.
Jun Katahira, Katja Straesser, Takuya Saiwaki, Yoshihiro Yoneda, and Ed Hurt (2002)
Modular Self-Assembly of a Y-Shaped Multiprotein Complex from Seven Nucleoporins. EMBO J., 21, No. 3, 387-397.
Malik Lutzmann, Ruth Kunze, Andrea Buerer, Ueli Aebi and Ed Hurt (2002)
Nuclear export of tRNA. Results Probl. Cell Differ. 35, 115-31.
Simos G, Grosshans H, Hurt E. (2002)
Rlp7p is associated with 60S pre-ribosomes, restricted to the granular component of the nucleolus and required for pre-rRNA processing. J. Cell Biol. in press.
Olivier Gadal, Daniela Strauß, Elisabeth Petfalski, Pierre-Emmanuel Gleizes, Nicole Gas, David Tollervey, and Ed Hurt (2002)
Structure of the C-terminal FG-Nucleoporin binding domain of Tap/NXF1. Nat. Struct. Biol. 9, 247-251. (reprints not available)
Richard Grant , Ed Hurt, David Neuhaus and Murray Stewart (2002)
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. Embo J. 21(21): 5843-5852. https://doi.org/10.1093/emboj/cdf590 Fischer, T., Sträßer, K., Rácz, A., Rodriguez-Navarro, S., Oppizzi, M., Ihrig, P., Lechner, J., & Hurt, E. (2002)
TREX is a conserved complex coupling transcription with mRNA export. Nature 417, 304-308.
Katja Sträßer, Seiji Masuda, Paul Mason, Jens Pfannstiel, Marisa Oppizzi, Susana Rodriguez-Navarro, Ana G. Rondón, Andres Aguilera, Kevin Struhl, Robin Reed and Ed Hurt (2002)
A nuclear AAA-type ATPase (Rix7p) is required for large ribosomal subunit biogenesis and subsequent export to the cytoplasm. EMBO J., 20, 3695-3704.
Olivier Gadal, Daniela Strauß, Joris Braspenning, Dominic Hoepfner, Elisabeth Petfalski, Peter Philippsen, David Tollervey and Ed Hurt (2001)
Arc1p Organizes the Yeast Aminoacyl-tRNA Synthetase Complex and Stabilizes Its Interaction with the Cognate tRNAs. J. Biol. Chem. 276, No. 8, February, 6000-6008.
Karina Deinert, Franco Fasiolo, Eduard C. Hurt and George Simos (2001)
Biogenesis of the Signal Recognition Particle Requires Nuclear Import of SRP proteins, Assembly with the SRP-RNA in the Nucleolus, and Xpo1p-mediated Nuclear Export. J. Cell Biol. 153, 745-62.
Helge Großhans, Karina Deinert, Ed Hurt and George Simos (2001)
Elongation factor like-1, a cytoplasmic GTPase homologous to the ribosomal translocases EF-G/EF-2 is required for ribosome export and a late cytoplasmic step of ribosomal assembly. Molecular Cell, 8, 1363-1373.
B. Senger, D. Lafontaine, O. Gadal, J. Graindorge, A. Camasses, A.Sanni, J. Garnier, M. Breitenbach, E. Hurt, and F. Fasiolo (2001)
Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell 8:517-29. Bassler, J., Grandi, P., Gadal, O., Lessmann, T., Petfalski, E., Tollervey, D., Lechner, J., & Hurt, E. (2001)
Maturation of Pre-Ribosomes Requires Noc-Proteins and is Coupled to Transport from the Nucleolus to the Nucleoplasm. Cell 105, 499-509. Philipp Milkereit, Olivier Gadal, Alexander Podtelejniko V., Stephanie Trumtel, Nicole Gas, Elisabeth Petfalski, David Tollervey, Mathias Mann, Ed Hurt and Herbert Tschochner (2001)
Mutations in the YRB1 Gene Encoding Yeast Ran-Binding-Protein-1 That Impair Nucleocytoplasmic Transport and Suppress Yeast Mating Defects. Genetics 157, 1089-1105.
Künzler M., Trueheart J., Sette C., Hurt E., Thorner J. (2001)
Nmd3p functions as a nuclear adaptor for Xpo1p-dependent export of 60S ribosomal subunits. Mol. Cell. Biol. 21, 3405-3415.
Olivier Gadal, Daniela Strauss, Jacques Kessl, Bernard Trumpower, David Tollervey and Ed Hurt (2001)
Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is compromised in yeast. J. Biol. Chem. 276, 46333-46339.
Helge Großhans, Francois Lecointe, Henri Grosjean Ed Hurt, and George Simos (2001)
Regulation of intracellular location of aminoacyl-tRNA-synthetases by complex formation with Arc1p. EMBO J. 20, 6889-6898.
K. Galani, H. Großhans, Deinert, K., E. C. Hurt,and G. Simos (2001)
Targeting of Ran: variation on a common theme? J. Cell Sci. 114, 3233-3241.
Markus Künzler and Ed Hurt (2001)
The Nsp1p carboxy-terminal domain is organized into functionally distinct coiled-coil regions required for assembly of nucleoporin subcomplexes and nucleocytoplasmic transport. Mol. Cell. Biol., 21, 7944-7955.
Susanne M. Bailer, Carolin Balduf, and Ed Hurt (2001)
The splicing factor Sub2p interacts directly with the mRNA transport factor Yra1p and is required for nuclear mRNA export. Nature 413, 648-652.
Katja Sträßer and Ed Hurt (2001)
A Generic Strategy to Analyze the Spatial Organization of Multi-Protein Complexes by Cross-Linking and Mass Spectrometry. Anal Chem.72, 267-75.
Juri Rappsilber, Symeon Siniossoglou, Eduard C. Hurt and Matthias Mann (2000)
An aminoacylation-dependent nuclear tRNA export pathway in yeast. Genes &. Dev. 14, 830-840.
Grosshans, H., E.C. Hurt and G. Simos (2000)
Binding of the Mex67p/Mtr2p Heterodimer to FXFG-, GLFG-, and FG-Repeat Nucleoporins is essential for Nuclear mRNA Export. J. Cell Biol., 150, 695-706.
Katja Sträßer, Jochen Bassler and Ed Hurt (2000)
Cloning and characterization of the Schizosaccharomyces pombe tRNA: pseudouridine synthase Pus1p. Nucleic Acids Research, Vol. 28, No. 23, 4604-4610.
Klaus Hellmuth, Henri Grosjean, Yuri Motorin, Karina Deinert, Ed Hurt and George Simos (2000)
Comparative spatial localization of Protein-A-tagged and authentic yeast nuclear pore complex proteins by immunogold electron microscopy. J. Struct. Biol.129, 295-305.
Birthe Fahrenkrog, John Aris, Ed Hurt, Nelly Panté and Ueli Aebi (2000)
Mex67p Mediates the Nuclear Export of a Variety of Pol II Transcripts. J. Biol. Chem. 275, 8361-8368.
Ed Hurt, Katja Sträßer, Alexandra Segref, Nick Schlaich, Carlo Presutti, David Tollervey and Ralf Jansen (2000)
Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with Nic96p. J. Biol. Chem. 275:343-350.
Kosova, B., N. Panté, C.Rollenhagen, A. Podtelejnikov, M. Mann, U. Aebi and E. Hurt (2000)
Nup116p associates with the Nup82p-Nsp1p-Nup159p nucleoporin complex. J. Biol. Chem. 275, 23540-23548.
Susanne Bailer, C. Balduf, J. Katahira, A. Podtelejnikov, Ch. Rollenhagen, M. Mann, N. Panté and Ed Hurt (2000)
Psr1p/Psr2p, two plasma membrane phosphatases with an essential DXDX(T/V) motif required for sodium stress response in yeast. J. Biol. Chem. 275, 19352-19360.
Symeon Siniossoglou, Ed Hurt and Hugh R. B. Pelham (2000)
Purification of ProteinA-Tagged Yeast Ran Reveals Association with a Novel Karyopherin beta Family Member Pdr6p. J. Biol. Chem. 275, 467-471. Denise Lau, Markus Künzler, Andreas Braunwarth, Klaus Hellmuth, Alexandre Podtelejnikov, Matthias Mann and Ed Hurt (2000)
Structure and Assembly of the Nup84p Complex. J. Cell. Biol. 149, 41-53.
Symeon Siniossoglou, Malik Lutzmann, Helena Santos-Rosa, Kevin Leonard, Shirley Mueller, Ueli Aebi and Ed Hurt (2000)
The protein ALY kinks pre-messenger-RNA splicing to nuclear export in metazoans. Nature, 407, 401-405.
Zhaolan Zhou, Ming-juan Luo, Katja Sträßer, Jun Katahira, Ed Hurt and Robin Reed (2000)
Transport of tRNA Out of the Nucleus - Direct Channeling to the Ribosome? J. Struct. Biol.129, 288-294.
Helge Grosshans, George Simos and Ed Hurt (2000)
Yeast Nuclear Pore Complex Assembly Defects Determined by Nuclear Envelope Reconstruction. J. Struct. Biol., Vol. 132, No. 1, October 2000, 1-5.
Natalia Gomez-Ospina, Garry Morgan, Thomas H. Giddings Jr., Buket Kosova, Ed Hurt and Mark Winey (2000)
Yeast Ran-binding protein 1 (Yrb1) shuttles between nucleus and cytoplasm and is exported from the nucleus via a CRM1 (XPO1)-dependent pathway. Mol. Cell Biol. 20, 4295-4308.
Markus Künzler, Thomas Gerstberger, Francoise Stutz, and Ed Hurt (2000)
Yra1p, a conserved nuclear RNA-binding protein, interacts directly with Mex67p and is required for mRNA export. EMBO J. 19, 410-420.
Katja Sträßer and Ed Hurt (2000)
A Novel In Vivo Assay Reveals Inhibition of Ribosomal Nuclear Export in Ran-Cycle and Nucleoporin Mutants. J. Cell Biol. 144(3):389-401.
Hurt, E.C., S. Hannus, B. Schmelzl, D. Lau, D. Tollervey and G. Simos (1999)
Nuclear export of mRNA in yeast. FEBS Lett. 452:77 - 81.
Sträßer, K., and E.C. Hurt (1999)
Nup192p is a Conserved Nucleoporin with a Preferential Location at the Inner Site of the Nuclear Membrane. J. Biol. Chem. 274:22646 - 22651.
Kosova, B., N. Panté, C. Rollenhagen, and E.C. Hurt (1999)
Pseudouridine Mapping in the Yeast spliceosomal UsnRNAs Reveals that Pseudouridine Synthase Pus1p Exhibits a Dual Substrate Specificity for U2snRNA and tRNA. Mol. Cell. Biol. 19:2142 - 2154.
Massenet, S., Y. Motorin, E. Hurt, H. Grosjean and C. Branlant (1999)
The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J., 18:2593-2609.
Katahira, J., K. Sträßer, A. Podtelejnikov, M. Mann, J. U. Jung, and E. C. Hurt (1999)
tRNA aminoacylation - a visa to leave the nucleus. Curr. Biol. 9:R238 - R241.
Simos, G., and E.C. Hurt (1999)
'Rush Hour' am Zellkern. Ruperto Carola 1, 12 -17.
E. Hurt (1998)
A Novel Complex of Membrane Proteins Required for Formation of a Spherical Nucleus. EMBO J. 17, 6449-6464.
S. Siniossoglou, H. Santos-Rosa, J. Rappsilber, M. Mann and E. Hurt (1998)
A tRNA-binding domain within Arc1p delivers tRNA to two aminoacyl-tRNA synthetases. Molecular Cell 1, 235 - 242.
G. Simos, A. Sauer, F. Fasiolo, and E.C. Hurt (1998)
Affinity Purification of Protein A-Tagged Nuclear Pore Proteins from Yeast. Cell Biology A Laboratory Hand Book. Academic Press. Second Edition. Vol. 2, 159 - 164.
S. Siniossoglou, P. Grandi, and E.C. Hurt (1998)
Characterization of yeast protein Deg1 as Pseudouridine synthase (Pus3) catalyzing the formation of Y38 and Y39 in tRNA anticodon loop. J. Biol. Chem. 273, 1316-1323.
F. Lecointe, G. Simos, A.Sauer, E.C. Hurt, Y. Motorin, and H. Grosjean (1998)
Cse1p functions as the nuclear export receptor for importin alpha in yeast. FEBS Lett., 433, 185-190.
M. Künzler and E.C. Hurt (1998)
Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes. J. Cell Biol. 143, 577 ? 588.
B. Fahrenkrog, E.C. Hurt, U. Aebi, and N. Panté (1998)
Mtr10p Functions as a Nuclear Import Receptor for the mRNA Binding Protein Npl3p. EMBO J. 17, 2196-2207.
B. Senger, G. Simos, F.R. Bischoff, A. Podtelejnikov, M. Mann, and E.C. Hurt (1998)
Nuclear compartments and nuclear machines. Curr. Opin. Cell Biol. 10, 301 - 303.
E. Hurt and G. Schütz (1998)
Nuclear mRNA Export Requires Complex Formation between Mex67p and Mtr2p at the Nuclear Pores. Mol. Cell. Biol. 18(11) 6826-6838.
H. Santos-Rosa, H. Moreno, G. Simos, A. Segref, B. Fahrenkrog, N. Panté, and E.C. Hurt (1998)
Nup116p and Nup100p are Interchangeable through a Conserved Motif which Constitutes a Binding Site for the mRNA Transport Factor Gle2p/Nup40p. EMBO J. 17, 1107-1119.
S. Bailer, S. Siniossoglou, S. Podtelejnikov, A. Hellwig, M. Mann, and E.C. Hurt (1998)
Yeast Los1p Has Properties of an Exportin-Like Nucleocytoplasmic Transport Factor for tRNA. Mol. Cell. Biol. 18(11) 6374-6386.
K. Hellmuth, D. Lau, R. Bischoff, M. Künzler, E.C. Hurt, and G. Simos (1998)
Yeast tRNA: pseudouridine synthase (Pus1p) displays a multisite substrate specificity. RNA 4, 856 - 869.
Y. Motorin, G. Keith, C. Simon, D. Foiret, G. Simos, E. Hurt, and H. Grosjean (1998)
From Nucleoporins to Functional Nuclear Pore Complexes. Curr. Opin. Cell Biol.9, 401 - 411.
V. Doye, and E.C. Hurt (1997)
In Vitro Reconstitution of a Heterotrimeric Nucleoporin Complex Consisting of Recombinant Nsp1p, Nup49p and Nup57p. Mol. Biol. Cell 8,33-46.
N.L. Schlaich, M. Häner, U. Aebi, and E.C. Hurt (1997)
Mex67p, A Novel Factor for Nuclear mRNA Export, Binds to Both Poly(A)+ RNA and Nuclear Pores. EMBO J. 11, 3256 - 3271.
A. Segref, K. Sharma, V. Doye, A. Hellwig, J. Huber, R. Lührmann, and E.C. Hurt (1997)
Nucleolar KKE/D Repeat Proteins Nop56p and Nop58p Interact with Nop1p and Are Required for Ribosome Biogenesis. Mol. Cell. Biol. 17, 7088-7098.
T. Gautier, T. Bergès, D. Tollervey, and E.C. Hurt (1997)
Nup93a, a Vertebrate Homolog of Yeast Nic96p Form a Complex with a Novel 205 kDa Protein and Are Involved in Nuclear Pore Assembly and Nuclear Growth. Mol. Biol. Cell. 8, 2017 - 2038.
P. Grandi, T. Dang, N. Panti, A. Shevchenko, M. Mann, D. Forbes, and E.C. Hurt (1997)
Two Functionally Distinct Domains Generated by in vivo Cleavage of Nup145p: a Novel Biogenesis Pathway for Nucleoporins. EMBO J. 16, 5086 - 5097.
M.T. Teixeira, S. Siniossoglou, S. Podtelejnikov, J.-C. Binichou, M. Mann, E.C. Hurt, and E. Fabre (1997)
Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Ann. Rev. Genet. 31, 277-313.
E. Fabre, and E.C. Hurt (1997)
A novel complex of nucleoporins, which includes Sec13p and Sec13p homolog, is essential for normal nuclear pores. Cell 84, 265 - 275.
S. Siniossoglou, C. Wimmer, M. Rieger, V. Doye, H. Tekotte, C. Weise, S. Emig, A. Segref, and E.C. Hurt (1996)
Analysis of Nic96p Reveals that its Central Domain is Required for Nuclear Pore Formation and Together with its Carboxy-Terminal Domain Functionally Interacts with a Novel Nucleoporin Nup188p. J. Cell Biol. 133, 1141-1152.
U. Zabel, V. Doye, H. Tekotte, R. Wepf, and E.C. Hurt (1996)
Importins/karyopherins meet nucleoporins. Cell 84, 509-515.
E.C. Hurt (1996)
MHP1, an essential gene in Saccharomyces cervisiae required for microtubule function. J. Cell Biology 135, 1323 - 1340.
I. Irminger-Finger, E.C. Hurt, A. Roebuck, M. A. Collart, and S.J. Edelstein (1996)
NBP35 encodes an essential and evolutionary conserved protein in Saccharomyces cerevisiae with homology to a superfamily of bacterial ATPases. Gene 178, 97-106.
G. Vitale, E. Fabre, and E.C. Hurt (1996)
Nuclear Pore Proteins are Required for the Biogenesis of Functional tRNA. EMBO J. 15, 2285-2290.
G. Simos, H. Tekotte, H. Grosjean, A. Segref, K. Sharma, D. Tollervey, and E.C. Hurt (1996)
The Yeast Protein Arc1p binds to tRNA and functions as a Cofactor for the Methionyl- and Glutamyl-tRNA Synthetases. EMBO J. 15, 5437 - 5448.
G. Simos, A. Segref, F. Fasiolo, K. Hellmuth, A. Shevchenko, M. Mann, and E.C. Hurt (1996)
Yeast nucleoporin mutants are defective in pre-tRNA splicing. Mol. Cell. Biol. 16, 294-301.
K. Sharma, E. Fabre, H. Tekotte, E.C. Hurt, and D. Tollervey (1996)
A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J. Cell Biol. 130, 1263-1273.
P. Grandi, S. Emig, C. Weise, F. Hucho, T. Pohl, and E.C. Hurt (1995)
Disruption of the gene coding for the nuclear pore protein Nup1p leads to nuclear accumulation of poly(A)+ RNA. Eur. J. Cell Biol. 67, 8 -14.
N.L. Schlaich, and E.C. Hurt (1995)
Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p. EMBO J. 14, 76 - 87.
P. Grandi, N.L. Schlaich, H. Tekotte, and E.C. Hurt (1995)
Genetic approaches to nuclear pore structure and function. Trends Genet. 11, 193-199.
V. Doye, and E.C. Hurt (1995)
Nucleocytoplasmic transport - factors and mechanisms. FEBS Lett. 369, 107-112.
G. Simos, and E.C. Hurt (1995)
The nuclear pore complex in yeast. In Membrane Protein Transport. Editor S. S. Rothman, Jai Press Ltd., Vol. 2, 29-56.
P. Grandi, and E.C. Hurt (1995)
A novel nuclear pore protein Nup133p with Distinct Roles in poly(A)+ RNA Transport and Nuclear Pore Distribution. EMBO J. 13, 6062 - 6075.
V. Doye, R. Wepf, and E.C. Hurt (1994)
Nuclear transport. Curr. Opin. Cell Biol. 6, 335-342.
E. Fabre, and E.C. Hurt (1994)
Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif. Cell 78, 275 - 289.
E. Fabre, W.C. Boelens, C. Wimmer, I. Mattaj, and E.C. Hurt (1994)
Synthetic lethality with fibrillarin idenitifies NOP77p, a nucleolar protein required for pre-rRNA pprocessing and modification. EMBO J. 13, 3136-3148.
T. Berges, E. Petfalski, D. Tollervey, and E.C. Hurt (1994)
A U3 snoRNP Protein with Homology to Splicing Factor PRP4 and Gb Domains is Required for Ribosomal RNA Processing. EMBO J. 12, 2549-2558.
R. P. Jansen, D. Tollervey, and E.C. Hurt (1993)
Analysis of nucleo-cytoplasmic transport in a thermo-sensitive mutant of nuclear pore protein NSP1. Eur. J. Cell Biol. 62, 1-12.
U. Nehrbass, E. Fabre, S. Dihlmann, W. Herth, and E.C. Hurt (1993)
Approaches towards a genetic analysis of the nuclear pore complex in yeast. In 'Protein Synthesis and Targeting' (eds. A.J.P. Brown, M.F. Tuite and J.E.G. McCarthy). NATO ASI Series, Vol. H 71, pp. 269-281, Springer-Verlag, Berlin-Heidelberg.
C. Wimmer, V. Doye, U. Nehrbass, N. L. Schlaich, and E.C. Hurt (1993)
Purification of NSP1 reveals complex formation with 'GLFG' nucleoporins and a novel nuclear pore protein NIC96. EMBO J. 12, 3061-3071.
P. Grandi, V. Doye, and E.C. Hurt (1993)
Reconstitution of nuclear protein transport with semi-intact yeast cells. J. Cell Biol. 123, 785-798.
G. Schlenstedt, E.C. Hurt, V. Doye, and P.A. Silver (1993)
Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. Cell 72, 443-457.
D. Tollervey, H. Lehtonen, R. Jansen, H. Kern, and E.C. Hurt (1993)
The nuclear pore complex. FEBS Lett. 325, 76-80.
E.C. Hurt (1993)
A New Subclass of Nucleoporins that Functionally Interacts with Nuclear Pore Protein NSP1. EMBO J. 11, 5051-5061.
C. Wimmer, V. Doye, P. Grandi, U. Nehrbass, and E.C. Hurt (1992)
Depletion of the nucleoporin NSP1 in yeast inhibits nuclear pore formation and nuclear transport of a nuclear reporter protein. Eur. J. Biol. 59, 280-295.
A. Mutvei, S. Dihlmann, W. Herth, and E.C. Hurt (1992)
Nuclear transport and nuclear pores in yeast. Int. J. Gen. Mol. Microbiol. 62, 3-14.
U. Nehrbass, and E. C. Hurt (1992)
The nuclear envelope of the yeast Saccharomyces cerevisiae. Int. Rev. Cytol. 136, 145-184.
E.C. Hurt, A. Mutvei, and M. Carmo-Fonseca (1992)
Across the nuclear pores with the help of nucleoporins. Chromosoma 101, 199-205.
E.C. Hurt and M. Carmo-Fonseca (1991)
Evolutionary conservation of the human protein fibrillarin and its functional expression in yeast. J. Cell Biol. 113, 715-727.
R.P. Jansen, E.C. Hurt, H. Kern, H. Lehtonen, M. Carmo-Fonseca, B. Lapeyere, and D. Tollervey (1991)
Human nucleoporin p62 and the essential yeast nuclear pore protein NSP1 show sequence homology and a similar domain organization. Eur. J. Cell Biol. 55, 17-30.
M. Carmo-Fonseca, H. Kern and E.C. Hurt (1991)
Mammalian nuclei contain foci which are highly enriched in components of the pre-mRNA splicing machinery. EMBO J. 10, 195-206.
M. Carmo-Fonseca, D. Tollervey, R. Pepperkok, S.M.L. Barabino, A. Merdes, C. Brunner, P.D. Zamore, M.R. Green, E.C. Hurt, and A.I. Lamond (1991)
The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J. 10, 573-583.
D. Tollervey, H. Lehtonen, M. Carmo-Fonseca, and E.C. Hurt (1991)
NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain. Cell 61, 979-989.
U. Nehrbass, H. Kern, A. Mutvei, H. Horstmann, B. Marshallsay, and E. C. Hurt (1990)
Targeting of a cytosolic protein to the nuclear periphery. J. Cell Biol. 111, 2829-2837.
E.C. Hurt (1990)
The role of small nucleolar ribonucleoproteins in ribosome biogenesis. Mol. Biol. Report 14, 103-206.
D. Tollervey, and E. C. Hurt (1990)
A 40 kDa nuclear membrane protein changes its concentration and localization within the nuclear envelope of the yeast Saccharomyces cerevisiae in a cell cycle dependent manner. Eur. J. Cell Biol. 49, 33-41.
T. Schimmang and E. C. Hurt (1989)
A yeast nucleolar protein related to human fibrillarin is associated with small nucleolar RNA and essential for viability. EMBO J. 8, 4015-4025.
T. Schimmang, D. Tollervey, H. Kern, R. Frank and E. C. Hurt (1989)
NSP1, a yeast protein located at the nuclear periphery is required for the cell-cycle of Saccharomyces cerevisiae. J. Cell Sci. 12, 243-252.
E. C. Hurt (1989)
A novel nucleosceletal-like protein located at the nuclear periphery is required for the life cycle of Saccharomyces cerevisiae. EMBO J. 7, 4323-4334.
E.C. Hurt (1988)
Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae. Eur. J. Cell Biol. 46, 554-563.
E.C. Hurt, A. McDowell, and T. Schimmang (1988)
Collaborations
• Ueli Aebi, University of Basel, CH
• Olivier Gadal, Institut Pasteur Paris, F
• Johannes Lechner, University of Heidelberg (BZH), D
• Robin Reed, Harvard Medical School Boston, USA
• Georgios Simos, University of Thessaly, GR
• Irmi Sinning, University of Heidelberg (BZH), D
• David Tollervey, University of Edinburgh, UK
• Herbert Tschochner, University of Regensburg, D
Funding
Research grant (DFG) on formation, maturation and nuclear export of pre-ribosomal subunits;
Research grant (DFG) on reconstitution of the nuclear pore complex;
Advanced Grant (ERC) "GLOWSOME" on eucaryotic ribosome assembly.
HORIZON2020
Contact
Heidelberg University
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Office:
+49 6221 54-4173 Fax:
+49 6221 54-4369 E-Mail:
ed.hurt@bzh.uni-heidelberg.deSecretary: Andrea Schliwa
Office:
+49 6221 54-4183 Fax:
+49 6221 54-4369 E-Mail:
andrea.schliwa@bzh.uni-heidelberg.de