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Groupleader: Johannes Lechner
Johannes Lechner
Kinetochore and Chromosome Segregation / ESI MS Service
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Research
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The kinetochore is a specialized chromosomal structure that facilitates chromosome attachment and movement along spindle microtubules during chromosome segregation. Furthermore, the kinetochore communicates the actual state of its interaction with the spindle to a cell cycle regulating machinery. It is the first member of a signaling cascade that inhibits sister chromatid separation (the beginning of anaphase) if chromosomes lack a stable bi-polar spindle attachment. A defect in this checkpoint function has been correlated to human cancers.
My group analyses the protein composition and structure of the S. cerevisiae kinetochore by mass spectroscopy and the function of individual kinetochore proteins applying cell and molecular biological methods.
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Academic Training and Positions
| 1975-1981 | Study of Chemistry at the University of Regensburg |
| 1981-1985 | PhD Student at the University of Regensburg (Prof. Dr. M. Sumper) |
| 1985-1987 | Post-Doc. University of Regensburg (Prof. Dr. M. Sumper) |
| 1987-1990 | Post-Doc. University of California, Santa Barbara (Prof. Dr. J. Carbon) |
| 1991-1994 | Research assistent at the University of Regensburg |
| 1994 | Habilitation in Biochemistry |
| 1994-1999 | Senior research assistent at the University of Regensburg |
| since 1999 | Group leader at the Heidelberg University Biochemistry Center (BZH) |
Lab Members
Publications
PI(4,5)P2-dependent oligomerization of fibroblast growth factor 2 (FGF2) triggers the formation of a lipidic membrane pore implicated in unconventional secretion. The Journal of biological chemistry, 287(33), 27659–27669. https://doi.org/10.1074/jbc.M112.381939
Steringer, J. P., Bleicken, S., Andreas, H., Zacherl, S., Laussmann, M., Temmerman, K., Contreras, F. X., Bharat, T. A., Lechner, J., Müller, H. M., Briggs, J. A., García-Sáez, A. J., & Nickel, W. (2012)
Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion. Traffic 11(6):813-26. Ebert AD, Laußmann M, Wegehingel S, Kaderali L, Erfle H, Reichert J, Lechner J, Beer HD, Pepperkok R, Nickel W. (2010)
Mimicking Ndc80 phosphorylation triggers spindle assembly checkpoint signalling. EMBO J. 28(8):1099-110.
Kemmler S, Stach M, Knapp M, Ortiz J, Pfannstiel J, Ruppert T, Lechner J. (2009)
Stu1 inversely regulates kinetochore capture and spindle stability. Genes Dev. 23(23):2778-91.
Ortiz J, Funk C, Schäfer A, Lechner J. (2009)
wa H, Priest C, Lechner J, Pereira G, Schiebel E. The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J Cell Biol. 179(3):423-36.
Kemmler S, Stach M, Knapp M, Ortiz J, Pfannstiel J, Ruppert T, Lechner J. (2007)
Getting in touch with Candida albicans: the cell wall of a fungal pathogen. Curr Drug Targets. 7(4):505-512.
Sohn K, Schwenk J, Urban C, Lechner J, Schweikert M, Rupp S. (2006)
TEDS site phosphorylation of the yeast myosins I is required for ligand-induced but not for constitutive endocytosis of the G protein-coupled receptor Ste2p. J Biol Chem. 281(16):11104-14.
Grosshans BL, Grötsch H, Mukhopadhyay D, Fernández IM, Pfannstiel J, Idrissi FZ, Lechner J, Riezman H, Geli MI. (2006)
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Cell. 116(1):75-86. Rodriguez-Navarro S, Fischer T, Luo MJ, Antunez O, Brettschneider S, Lechner J, Perez-Ortin JE, Reed R, Hurt E. (2004)
The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association. Mol Biol Cell. 15(12):5255-67.
Kerres A, Vietmeier-Decker C, Ortiz J, Karig I, Beuter C, Hegemann J, Lechner J, Fleig U. (2004)
A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits. The Journal of Biological Chemistry, Vol. 278, No. 6, pp. 4072-4081. Milkereit, P., Strauss, D., Bassler, J., Gadal, O., Kühn, H., Schütz, S., Gas, N., Lechner, J., Hurt, E., & Tschochner, H. (2003)
Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. The EMBO journal, 22(24), 6584–6597. https://doi.org/10.1093/emboj/cdg636 Scharfenberger M, Ortiz J, Grau N, Janke C, Schiebel E, Lechner J. (2003)
The cancer antigen CA125 represents a novel counter receptor for galectin-1. J Cell Sci. 116(Pt 7):1305-18.
Seelenmeyer C, Wegehingel S, Lechner J, Nickel W. (2003)
Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J. 21(1-2):181-93.
Janke C, Ortíz J, Tanaka TU, Lechner J, Schiebel E. (2002)
Hsp90 enables Ctf13p/Skp1p to nucleate the budding yeast kinetochore. Biochemistry 99(13):8585-90.
Stemmann O, Neidig A, Köcher T, Wilm M, Lechner J. (2002)
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. Embo J. 21(21): 5843-5852. https://doi.org/10.1093/emboj/cdf590 Fischer, T., Sträßer, K., Rácz, A., Rodriguez-Navarro, S., Oppizzi, M., Ihrig, P., Lechner, J., & Hurt, E. (2002)
Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell 8:517-29. Bassler, J., Grandi, P., Gadal, O., Lessmann, T., Petfalski, E., Tollervey, D., Lechner, J., & Hurt, E. (2001)
Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23. The EMBO journal, 20(23), 6751–6760. https://doi.org/10.1093/emboj/20.23.6751 Gommel, D. U., Memon, A. R., Heiss, A., Lottspeich, F., Pfannstiel, J., Lechner, J., Reinhard, C., Helms, J. B., Nickel, W., & Wieland, F. T. (2001)
The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. The EMBO journal, 20(4), 777–791. https://doi.org/10.1093/emboj/20.4.777 Janke, C., Ortiz, J., Lechner, J., Shevchenko, A., Shevchenko, A., Magiera, M. M., Schramm, C., & Schiebel, E. (2001)
Interaction of yeast kinetochore proteins with centromere-protein/transcription factor Cbf1. Proc Natl Acad Sci USA. 97(23):12583-12588. doi:10.1073/pnas.97.23.12583 Hemmerich P, Stoyan T, Wieland G, Koch M, Lechner J, Diekmann S. (2000)
The budding yeast kinetochore: less simple than expected. Protoplasma 211:12-19.
Ortiz J, Lechner J. (2000)
A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev 13:1140-55.
Ortiz J, Stemmann O, Rank S, Lechner J. (1999)
Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata. Yeast 15:793-8.
Stoyan T, Eck R, Lechner J, Hemmerich P, Kunkel W, Diekmann S. (1999)
Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy. Proc Natl Acad Sci U S A 96:3757-62. Pietrasanta, L. I., Thrower, D., Hsieh, W., Rao, S., Stemmann, O., Lechner, J., Carbon, J., & Hansma, H. (1999)
The Saccharomyces cerevisiae kinetochore contains a cyclin-CDK complexing homologue, as identified by in vitro reconstitution. Embo J 15:3611-20.
Stemmann O, Lechner J. (1996)
The Saccharomyces cerevisiae kinetochore. FEBS Lett 389:70-4.
Lechner J, Ortiz J. (1996)
A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3. Embo J 13:5203-11.
Lechner J. (1994)
Analysis of bacterial glycoproteins. Methods Mol Biol 14:119-29. Lechner J, Wieland F. (1993)
Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore. J Cell Biol 121:513-9.
Jiang W, Lechner J, Carbon J. (1993)
A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere. Cell 64:717-25.
Lechner J, Carbon J. (1991)
Structure and biosynthesis of prokaryotic glycoproteins. Annu Rev Biochem 58:173-94.
Lechner J, Wieland F. (1989)
The primary structure of a procaryotic glycoprotein. Cloning and sequencing of the cell surface glycoprotein gene of halobacteria. J Biol Chem 262:9724-9.
Lechner J, Sumper M. (1987)
Iduronic acid is a constituent of sulfated dolichyl phosphate oligosaccharides in halobacteria. FEBS Lett. 195:77-81. Wieland F, Lechner J, Sumper M. (1986)
Sulfated Dolicholphosphate Oligosaccharides are Transiently Methylated during Biosynthesis of Halobacterial Glycoproteins. System. Appl. Microbiol. 7:286-392. Wieland F, Lechner J, Sumper M. (1986)
Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose. Purification and identification of sulfated dolichyl monophosphoryl tetrasaccharides from halobacteria. J Biol Chem 260:860-6. Lechner J, Wieland F, Sumper M. (1985)
Transient methylation of dolichyl oligosaccharides is an obligatory step in halobacterial sulfated glycoprotein biosynthesis. J Biol Chem 260:8984-9. Lechner J, Wieland F, Sumper M. (1985)
The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetiv Aspects. Zentralbl. Bacteriol. Mikrobiol. Hyg. 1 Abt. Orig. C 3,:161-170. Wieland F, Lechner J, Sumper M. (1982)
Sulfation of a repetitive saccharide in halobacterial cell wall glycoprotein. FEBS Lett 132:319-323. Wieland F, Lechner J, Bernhardt G, Sumper M. (1981)
Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion. Traffic 11(6):813-26. Ebert AD, Laußmann M, Wegehingel S, Kaderali L, Erfle H, Reichert J, Lechner J, Beer HD, Pepperkok R, Nickel W. (2010)
Mimicking Ndc80 phosphorylation triggers spindle assembly checkpoint signalling. EMBO J. 28(8):1099-110.
Kemmler S, Stach M, Knapp M, Ortiz J, Pfannstiel J, Ruppert T, Lechner J. (2009)
Stu1 inversely regulates kinetochore capture and spindle stability. Genes Dev. 23(23):2778-91.
Ortiz J, Funk C, Schäfer A, Lechner J. (2009)
wa H, Priest C, Lechner J, Pereira G, Schiebel E. The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. J Cell Biol. 179(3):423-36.
Kemmler S, Stach M, Knapp M, Ortiz J, Pfannstiel J, Ruppert T, Lechner J. (2007)
Getting in touch with Candida albicans: the cell wall of a fungal pathogen. Curr Drug Targets. 7(4):505-512.
Sohn K, Schwenk J, Urban C, Lechner J, Schweikert M, Rupp S. (2006)
TEDS site phosphorylation of the yeast myosins I is required for ligand-induced but not for constitutive endocytosis of the G protein-coupled receptor Ste2p. J Biol Chem. 281(16):11104-14.
Grosshans BL, Grötsch H, Mukhopadhyay D, Fernández IM, Pfannstiel J, Idrissi FZ, Lechner J, Riezman H, Geli MI. (2006)
Sus1, a functional component of the SAGA histone acetylase complex and the nuclear pore-associated mRNA export machinery. Cell. 116(1):75-86. Rodriguez-Navarro S, Fischer T, Luo MJ, Antunez O, Brettschneider S, Lechner J, Perez-Ortin JE, Reed R, Hurt E. (2004)
The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association. Mol Biol Cell. 15(12):5255-67.
Kerres A, Vietmeier-Decker C, Ortiz J, Karig I, Beuter C, Hegemann J, Lechner J, Fleig U. (2004)
A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits. The Journal of Biological Chemistry, Vol. 278, No. 6, pp. 4072-4081. Milkereit, P., Strauss, D., Bassler, J., Gadal, O., Kühn, H., Schütz, S., Gas, N., Lechner, J., Hurt, E., & Tschochner, H. (2003)
Nsl1p is essential for the establishment of bipolarity and the localization of the Dam-Duo complex. The EMBO journal, 22(24), 6584–6597. https://doi.org/10.1093/emboj/cdg636 Scharfenberger M, Ortiz J, Grau N, Janke C, Schiebel E, Lechner J. (2003)
The cancer antigen CA125 represents a novel counter receptor for galectin-1. J Cell Sci. 116(Pt 7):1305-18.
Seelenmeyer C, Wegehingel S, Lechner J, Nickel W. (2003)
Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J. 21(1-2):181-93.
Janke C, Ortíz J, Tanaka TU, Lechner J, Schiebel E. (2002)
Hsp90 enables Ctf13p/Skp1p to nucleate the budding yeast kinetochore. Biochemistry 99(13):8585-90.
Stemmann O, Neidig A, Köcher T, Wilm M, Lechner J. (2002)
The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores. Embo J. 21(21): 5843-5852. https://doi.org/10.1093/emboj/cdf590 Fischer, T., Sträßer, K., Rácz, A., Rodriguez-Navarro, S., Oppizzi, M., Ihrig, P., Lechner, J., & Hurt, E. (2002)
Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell 8:517-29. Bassler, J., Grandi, P., Gadal, O., Lessmann, T., Petfalski, E., Tollervey, D., Lechner, J., & Hurt, E. (2001)
Recruitment to Golgi membranes of ADP-ribosylation factor 1 is mediated by the cytoplasmic domain of p23. The EMBO journal, 20(23), 6751–6760. https://doi.org/10.1093/emboj/20.23.6751 Gommel, D. U., Memon, A. R., Heiss, A., Lottspeich, F., Pfannstiel, J., Lechner, J., Reinhard, C., Helms, J. B., Nickel, W., & Wieland, F. T. (2001)
The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. The EMBO journal, 20(4), 777–791. https://doi.org/10.1093/emboj/20.4.777 Janke, C., Ortiz, J., Lechner, J., Shevchenko, A., Shevchenko, A., Magiera, M. M., Schramm, C., & Schiebel, E. (2001)
Interaction of yeast kinetochore proteins with centromere-protein/transcription factor Cbf1. Proc Natl Acad Sci USA. 97(23):12583-12588. doi:10.1073/pnas.97.23.12583 Hemmerich P, Stoyan T, Wieland G, Koch M, Lechner J, Diekmann S. (2000)
The budding yeast kinetochore: less simple than expected. Protoplasma 211:12-19.
Ortiz J, Lechner J. (2000)
A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore. Genes Dev 13:1140-55.
Ortiz J, Stemmann O, Rank S, Lechner J. (1999)
Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata. Yeast 15:793-8.
Stoyan T, Eck R, Lechner J, Hemmerich P, Kunkel W, Diekmann S. (1999)
Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy. Proc Natl Acad Sci U S A 96:3757-62. Pietrasanta, L. I., Thrower, D., Hsieh, W., Rao, S., Stemmann, O., Lechner, J., Carbon, J., & Hansma, H. (1999)
The Saccharomyces cerevisiae kinetochore contains a cyclin-CDK complexing homologue, as identified by in vitro reconstitution. Embo J 15:3611-20.
Stemmann O, Lechner J. (1996)
The Saccharomyces cerevisiae kinetochore. FEBS Lett 389:70-4.
Lechner J, Ortiz J. (1996)
A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3. Embo J 13:5203-11.
Lechner J. (1994)
Analysis of bacterial glycoproteins. Methods Mol Biol 14:119-29. Lechner J, Wieland F. (1993)
Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore. J Cell Biol 121:513-9.
Jiang W, Lechner J, Carbon J. (1993)
A 240 kd multisubunit protein complex, CBF3, is a major component of the budding yeast centromere. Cell 64:717-25.
Lechner J, Carbon J. (1991)
Structure and biosynthesis of prokaryotic glycoproteins. Annu Rev Biochem 58:173-94.
Lechner J, Wieland F. (1989)
The primary structure of a procaryotic glycoprotein. Cloning and sequencing of the cell surface glycoprotein gene of halobacteria. J Biol Chem 262:9724-9.
Lechner J, Sumper M. (1987)
Iduronic acid is a constituent of sulfated dolichyl phosphate oligosaccharides in halobacteria. FEBS Lett. 195:77-81. Wieland F, Lechner J, Sumper M. (1986)
Sulfated Dolicholphosphate Oligosaccharides are Transiently Methylated during Biosynthesis of Halobacterial Glycoproteins. System. Appl. Microbiol. 7:286-392. Wieland F, Lechner J, Sumper M. (1986)
Biosynthesis of sulfated saccharides N-glycosidically linked to the protein via glucose. Purification and identification of sulfated dolichyl monophosphoryl tetrasaccharides from halobacteria. J Biol Chem 260:860-6. Lechner J, Wieland F, Sumper M. (1985)
Transient methylation of dolichyl oligosaccharides is an obligatory step in halobacterial sulfated glycoprotein biosynthesis. J Biol Chem 260:8984-9. Lechner J, Wieland F, Sumper M. (1985)
The Cell Wall Glycoprotein of Halobacteria: Structural, Functional and Biosynthetiv Aspects. Zentralbl. Bacteriol. Mikrobiol. Hyg. 1 Abt. Orig. C 3,:161-170. Wieland F, Lechner J, Sumper M. (1982)
Sulfation of a repetitive saccharide in halobacterial cell wall glycoprotein. FEBS Lett 132:319-323. Wieland F, Lechner J, Bernhardt G, Sumper M. (1981)
Collaborations
Dr. Elmar Schiebel
Cancer Research UK Paterson Laboratories
Christie Hospital NHS Trust
Wilmslow Road
Manchester M20 4BX
UK
Funding
DFG Einzelantrag
Contact
Heidelberg University
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Office:
+49 6221 54-4371 Lab:
+49 6221 54-5424 Fax:
+49 6221 54-5586 E-Mail:
johannes.lechner@bzh.uni-heidelberg.de