RESEARCH GROUPS
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Groupleader: Mandy Jeske
Mandy Jeske
Molecular Functions of Germline Proteins
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Research
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In particular, we are currently focusing ours studies on LOTUS domain-containing proteins and on RNA helicases. To unravel the molecular mechanisms of how these proteins function, we employ a multidisciplinary approach combining RNA/protein biochemistry, X-ray crystallography, cell culture techniques as well as Drosophila genetics.
Twitter @Jeske_Lab
CV
| 1998 - 2005 | Biochemistry Studies at University Halle-Wittenberg |
| 2005 - 2010 | PhD at the University Halle-Wittenberg with Elmar Wahle |
| 2010 - 2017 |
Postdoc at the EMBL with Anne Ephrussi and Christoph W. Müller (EIPOD Developmental/Structural biology) |
| since 2017 | Emmy Noether Group Leader at the Heidelberg University Biochemistry Center (BZH) |
Lab Members
Asen Garbelyanski
PhD student
PhD student
Lab:Room 422
/ Phone: +49 6221 54-4739
Mail:asen.garbelyanski@bzh.uni-heidelberg.de
Jana Kubikova
PhD student
PhD student
Lab:Room 422
/ Phone: +49 6221 54-4739
Mail:jana.kubikova@bzh.uni-heidelberg.de
Jutta Metz
TA
TA
Lab:Room 422
/ Phone: +49 6221 54-6894
Mail:jutta.metz@bzh.uni-heidelberg.de
Harpreet Kaur Salgania
PhD student
PhD student
Lab:Room 422
/ Phone: +49 6221 54-6894
Mail:harpreet.salgania@bzh.uni-heidelberg.de
Publications
ReLo: a simple colocalization assay to identify and characterize physical protein-protein interactions. BioRxiv. doi: https://doi.org/10.1101/2022.03.04.482790
Salgania, H.K., Metz, J., and Jeske, M. (2022)
RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system. BioRxiv. doi:https://doi.org/10.1101/2022.05.11.491288
Pekovic, F., Rammelt, C., Kubíková, J., Metz, J., Jeske, M., Wahle, E. (2022)
Molekulare Funktionen von Keimbahn-spezifischen Proteinen. BIOspektrum. 27, 101.
Jeske, M. (2021)
Molecular functions of germline proteins. EU Research. SPR21, 18.
Jeske, M. (2021)
LOTUS-domain proteins - Developmental effectors from a molecular perspective. Biol. Chem. 402, 7–23.
Kubíková, J., Reinig, R., Salgania, H.K., and Jeske, M. (2020)
The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage. Genes Dev. 31(9), 939-952.
Jeske, M., Müller, C.W., Ephrussi, A. (2017)
The crystal structure of the Drosophila germline-inducer Oskar identifies two domains with distinct Vasa helicase- and RNA-binding activities. Cell Rep. 12(4), 587-98.
Jeske, M., Bordi, M., Glatt, S., Müller, S., Rybin, V., Müller, C.W., Ephrussi, A. (2015)
Assaying mRNA deadenylation in vitro. Methods Mol. Biol. 1125, 297–311.
Jeske, M., Temme, C., Wahle, E. (2014)
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J. Biol. Chem. 288(4), 2441-2451.
Buschmann, J., Moritz, B., Jeske, M., Lilie, H., Schierhorn, A., Wahle, E. (2013)
Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. EMBO J. 30(1), 90-103.
Jeske, M., Moritz, B., Anders, A., Wahle, E. (2011)
Cell-free deadenylation assays with Drosophila embryo extracts. Meth. Enzymol. 448, 107-118.
Jeske, M., Wahle, E. (2008)
Rapid ATP-dependent deadenylation of nanos mRNA in a cell-free system from Drosophila embryos. J. Biol. Chem. 281(35), 25124-25133.
Jeske, M., Meyer, S., Temme, C., Freudenreich, D., Wahle, E. (2006)
Salgania, H.K., Metz, J., and Jeske, M. (2022)
RNA binding proteins Smaug and Cup induce CCR4-NOT-dependent deadenylation of the nanos mRNA in a reconstituted system. BioRxiv. doi:https://doi.org/10.1101/2022.05.11.491288
Pekovic, F., Rammelt, C., Kubíková, J., Metz, J., Jeske, M., Wahle, E. (2022)
Molekulare Funktionen von Keimbahn-spezifischen Proteinen. BIOspektrum. 27, 101.
Jeske, M. (2021)
Molecular functions of germline proteins. EU Research. SPR21, 18.
Jeske, M. (2021)
LOTUS-domain proteins - Developmental effectors from a molecular perspective. Biol. Chem. 402, 7–23.
Kubíková, J., Reinig, R., Salgania, H.K., and Jeske, M. (2020)
The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage. Genes Dev. 31(9), 939-952.
Jeske, M., Müller, C.W., Ephrussi, A. (2017)
The crystal structure of the Drosophila germline-inducer Oskar identifies two domains with distinct Vasa helicase- and RNA-binding activities. Cell Rep. 12(4), 587-98.
Jeske, M., Bordi, M., Glatt, S., Müller, S., Rybin, V., Müller, C.W., Ephrussi, A. (2015)
Assaying mRNA deadenylation in vitro. Methods Mol. Biol. 1125, 297–311.
Jeske, M., Temme, C., Wahle, E. (2014)
Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J. Biol. Chem. 288(4), 2441-2451.
Buschmann, J., Moritz, B., Jeske, M., Lilie, H., Schierhorn, A., Wahle, E. (2013)
Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. EMBO J. 30(1), 90-103.
Jeske, M., Moritz, B., Anders, A., Wahle, E. (2011)
Cell-free deadenylation assays with Drosophila embryo extracts. Meth. Enzymol. 448, 107-118.
Jeske, M., Wahle, E. (2008)
Rapid ATP-dependent deadenylation of nanos mRNA in a cell-free system from Drosophila embryos. J. Biol. Chem. 281(35), 25124-25133.
Jeske, M., Meyer, S., Temme, C., Freudenreich, D., Wahle, E. (2006)
Collaborations
Funding
Open Positions
Interested Trainees and motivated Bachelor and Master students are always welcome to join the lab. Please contact me by mail: jeske@bzh.uni-heidelberg.de
Contact
Heidelberg University
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
Office:
+49 6221 54-4728 Fax:
+49 6221 54-4369 E-Mail:
jeske@bzh.uni-heidelberg.de