1. Research group April 2022
2. RNA helicases
3. Protein crystal
4. Electron density map
5. Drosophila egg chambers with Vasa staining
In the lab, we are using a multidisciplinary approach to investigate proteins and protein complexes with essential functions during germ cell formation and early embryonic development of Drosophila
. The research focusses on understanding molecular mechanism underlying piRNA biogenesis and piRNA-mediated transposon silencing of transposon mRNAs, processes which are highly critical during gametogenesis and early animal development.
In particular, we are currently focusing our studies on LOTUS domain-containing proteins (Kubíková et al., 2020) and on ATP-dependent RNA helicases. To unravel the molecular mechanisms of how these proteins function in the piRNA pathway, we combine RNA/protein biochemistry, X-ray crystallography, and cell culture techniques with Drosophila