Groupleader: Mandy Jeske
Mandy Jeske
Transposon Silencing and Translation Control
Research
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The LOTUS is a class of protein domains, which is highly conserved from bacteria to humans. Metazoan LOTUS domain proteins function in silencing of transposon mRNAs in the germline by the piRNA pathway as well as in translation control of mRNAs that are essential for early embryonic development. Loss of function results in infertility and/or embryonic patterning defects of the organism.
In the lab, we aim to unravel the molecular mechanisms of how LOTUS domain proteins function during germ cell specification and early embryonic development. To accomplish this goal, a multidisciplinary approach combining RNA/protein biochemistry, X-ray crystallography, and Drosophila genetics will be employed.
CV
| 1998 - 2005 | Biochemistry Student at Martin-Luther-University Halle-Wittenberg |
| 2005 - 2010 | Predoctoral Research Fellow at the Martin-Luther-University Halle-Wittenberg |
| 2010 - 2017 | Interdisciplinary Postdoctoral Research Fellow (EIPOD) at the EMBL |
| since 2017 | Emmy Noether Junior Group Leader at the Heidelberg University Biochemistry Center (BZH) |
Lab Members
PhD student
Lab:Room 422
/ Phone: +49 6221 54-4764
Mail:jana.kubikova@bzh.uni-heidelberg.de
TA
Lab:Room 422
/ Phone: +49 6221 54-6894
Mail:jutta.metz@bzh.uni-heidelberg.de
PhD student
Lab:Room 422
/ Phone: +49 6221 54-4764
Mail:rebecca.reinig@bzh.uni-heidelberg.de
PhD student
Lab:Room 422
/ Phone: +49 6221 54-6894
Mail:harpreet.salgania@bzh.uni-heidelberg.de
research assistant
Lab:Room 422
/ Phone: +49 6221 54-4764
Mail:pg234@stud.uni-heidelberg.de
Publications
Jeske, M., Müller, C.W., Ephrussi, A. (2017). The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage. Genes Dev. 31(9), 939-952.
http://genesdev.cshlp.org/content/31/9/939.long
Jeske, M., Bordi, M., Glatt, S., Müller, S., Rybin, V., Müller, C.W., Ephrussi, A. (2015). The crystal structure of the Drosophila germline-inducer Oskar identifies two domains with distinct Vasa helicase- and RNA-binding activities. Cell Rep. 12(4), 587-98.
http://www.cell.com/cell-reports/fulltext/S2211-1247(15)00681-6
Jeske, M., Temme, C., Wahle, E. (2014). Assaying mRNA deadenylation in vitro. Methods Mol. Biol. 1125, 297–311.
https://link.springer.com/protocol/10.1007%2F978-1-62703-971-0_24
Buschmann, J., Moritz, B., Jeske, M., Lilie, H., Schierhorn, A., Wahle, E. (2013). Identification of Drosophila and human 7-methyl GMP-specific nucleotidases. J. Biol. Chem. 288(4), 2441-2451.
http://www.jbc.org/content/288/4/2441.long
Jeske, M., Moritz, B., Anders, A., Wahle, E. (2011). Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. EMBO J. 30(1), 90-103.
http://emboj.embopress.org/content/30/1/90.long
Jeske, M., Wahle, E. (2008). Cell-free deadenylation assays with Drosophila embryo extracts. Meth. Enzymol. 448, 107-118.
http://www.sciencedirect.com/science/article/pii/S0076687908026062?via%3Dihub
Jeske, M., Meyer, S., Temme, C., Freudenreich, D., Wahle, E. (2006). Rapid ATP-dependent deadenylation of nanos mRNA in a cell-free system from Drosophila embryos. J. Biol. Chem. 281(35), 25124-25133.
http://www.jbc.org/content/281/35/25124.long
Collaborations
Funding
Open Positions
Interested Trainees and motivated Bachelor and Master students are always welcome to join the lab. Please contact me by mail: jeske@bzh.uni-heidelberg.de
Contact
Biochemistry Center (BZH)
Im Neuenheimer Feld 328
69120 Heidelberg
