Forschungsgruppen

Publikationen

Complete list of publications:

Walter Nickel at PubMed


New preprints on biorxiv.org

Nanoscale PI(4,5)P2 partitioning driven by FGF2 oligomerization triggers membrane pore formation
Alessandra Griffo, Daniel Beyer, Jaime Fernández Sobaberas, Melanie König, Zeynep Kilcan, Manpreet Kaur, Eleonora Margheritis, Katia Cosentino, ProfilePetra Riegerová, Martin Hof, Radek Šachl, Walter Nickel, Fabio Lolicato
doi: https://doi.org/10.1101/2025.09.11.675533

Plasma membrane transbilayer asymmetry of PI(4,5)P2 drives unconventional secretion of Fibroblast Growth Factor 2
Manpreet Kaur, Fabio Lolicato, Walter Nickel
doi: https://doi.org/10.1101/2025.04.19.649664


Key publications

Lolicato F, Steringer JP, Saleppico R, Beyer D, Fernandez-Sobaberas J, Unger S, Klein S, Riegerová P, Wegehingel S, Müller HM, Freund C, Hof, M, Šachl R, Chlanda P, Vattulainen I, and Nickel W (2023) Disulfide bridge-dependent dimerization triggers FGF2 membrane translocation into the extracellular space. eLife 88579

Lolicato F, Saleppico R, Griffo A, Meyer A, Scollo F, Pokrandt B, Müller HM, Ewers H, Hähl H, Fleury JB, Seemann R, Hof M, Brügger B, Jacobs K, Vattulainen I, Nickel W (2022) Cholesterol promotes clustering of PI(4,5)P2 driving unconventional secretion of FGF2. J Cell Biol e202106123

Sparn C, Dimou E, Meyer A, Saleppico R, Wegehingel S, Gerstner M, Klaus S, Ewers H, Nickel W (2022) Glypican-1 drives unconventional secretion of Fibroblast Growth Factor 2. eLife e75545

Legrand C, Saleppico R, Sticht J, Lolicato F, Müller HM, Wegehingel S, Dimou E, Steringer JP, Ewers E, Vattulainen I, Freund C, Nickel W (2020) The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2. Commun Biol 3:141

Dimou E, Cosentino K, Platonova E, Ros U, Sadeghi M, Kashyap P, Katsinelos T, Wegehingel S, Noé F, García-Sáez AJ, Ewers H, Nickel W (2019) Single event visualization of unconventional secretion of FGF2. J Cell Biol 218:683-699

Katsilenos T, Zeitler M, Dimou E, Karakatsani A, Müller HM, Nachman E, Steringer JP, Ruiz de Almodovar C, Nickel W#, Jahn TR#(2018) Unconventional secretion mediates the trans-cellular spreading of Tau. Cell Reports 23:2039-2055 (# corresponding authors)

Steringer JP, Lange S, Čujová S, Šachl R, Poojari C, Lolicato F, Beutel O, Müller HM, Unger S, Coskun Ü, Honigmann A, Vattulainen I, Hof M, Freund C, Nickel W (2017) Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components. eLife e28985

Müller HM, Steringer JP, Wegehingel S, Bleicken S, Münster M, Dimou E, Unger S, Weidmann G, Andreas H, García-Sáez AJ, Wild K, Sinning I, Nickel W (2015) Formation of disulfide bridges drives oligomerization, membrane pore formation, and translocation of fibroblast growth factor 2 to cell surfaces. J Biol Chem 290:8925-8937

Steringer JP, Bleicken S, Andreas H, Zacherl S, Laussmann M, Temmerman K, Contreras FX, Bharat TA, Lechner J, Müller HM, Briggs JA, García-Sáez AJ, Nickel W (2012) Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]-dependent oligomerization of fibroblast growth factor 2 (FGF2) triggers the formation of a lipidic membrane pore implicated in unconventional secretion.
J Biol Chem 287:27659-27669

Ebert AD, Laussmann M, Wegehingel S, Kaderali L, Erfle H, Reichert J, Lechner J, Beer HD, Pepperkok R, Nickel W (2010) Tec-kinase-mediated phosphorylation of fibroblast growth factor 2 is essential for unconventional secretion. Traffic 11:813-826

Zehe C, Engling A, Wegehingel S, Schäfer T, Nickel W (2006) Cell-surface heparan sulfate proteoglycans are essential components of the unconventional export machinery of FGF-2.
Proc. Natl. Acad. Sci. U.S.A. 103:15479-15484

Seelenmeyer C, Wegehingel S, Tews I, Künzler M, Aebi M, Nickel W (2005) Cell surface counter receptors are essential components of the unconventional export machinery of galectin-1.
J. Cell Biol. 17:373-381


Reviews on unconventional protein secretion

Sparn C, Meyer, A, Saleppico R, and Nickel W (2022) Unconventional secretion mediated by direct protein self-translocation across the plasma membranes of mammalian cells.
Trends Biochem Sci 47:699-709

Lolicato F and Nickel W (2022) A role for liquid-ordered plasma membrane nanodomains coordinating the unconventional secretory pathway of Fibroblast Groth Factor 2.
Front Cell Dev Biol 10:864257

Pallotta MT and Nickel W (2020) FGF2 and IL-1β: Explorers of unconventional secrtory pathways.
J Cell Sci 133(21):jcs250449

Dimou E and Nickel W (2018) Unconventional mechanisms of eukaryotic protein secretion.
Curr Biol 28:R406-R410